Use of High-Performance Liquid Chromatography in Characterization of Suspected Haemoglobinopathies in Central India-Clinicohaematological Correlation

Abstract

Context: Haemoglobin High Performance Liquid Chromatography is a technique for screening and initial diagnosis of haemoglobinopathies which includes group of disorders. Detailed clinical history, complete hematological prole along with newer ancillary techniques including Hb HPLC when used systematically and judiciously can achieve an early diagnosis. Family studies are of considerable importance in elucidating the nature of disorders. To aid in a cut off value for suspected haemoglobinopathies in Central India. To determine Haemoglobin prole inAims: suspected cases of Haemoglobinopathies in Central India. Hospital based retrospective and prospective study.Setting And Design: Method And Material: Blood samples in EDTA analyzed on cell counters. Red cell parameters and reticulocyte count were noted and peripheral smear evaluated. Screening tests and Serum Ferritin levels done. Samples were then run-on HPLC system. Youden's criteriaStatistical Analysis Used: was applied for calculation of cutoff values. ROC curve prepared wherever required along with calculations done on MS-EXCEL. TheResult: cutoff for beta thalassemia trait came out as HbA2>= 3.35% and HbS>8% for sickle cell trait amongst cases collected from Madhya Pradesh. Conclusion: Study ndings show HPLC as an excellent, powerful diagnostic tool for the direct identication of hemoglobin variants with a high degree of precision in the quantication of normal and abnormal hemoglobin fractions.