Use of high-throughput techniques to study simultaneous production of lipid metabolites in carotenogenic yeasts grown on waste animal fat

Márová, Ivana; Rapta, Marek; Vanek, Martin; Hároniková, Andrea; Szotkowski, Martin; Shapaval, Volha

doi:10.1016/j.jbiotec.2017.06.689

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“…Different processes have been proposed in literature for converting animal fat into high value products [4]. One of the proposed strategies is based on converting hydrophobic substrates (such as vegetable oils, volatile fatty acids, and animal fats) into valuable essential unsaturated single cell oils via fermentation by oleaginous microorganisms [3,[10][11][12][13][14][15][16][17][18]. Oleaginous microorganisms have the ability to accumulate high amount of lipids (from 20% up to 80% of their dry cellular weight) in the form of triglycerides (TGAs) and free fatty acids.…”

Section: Introductionmentioning

confidence: 99%

Submerged Fermentation of Animal Fat By-Products by Oleaginous Filamentous Fungi for the Production of Unsaturated Single Cell Oil

et al. 2021

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Animal waste fats were explored as a fermentation substrate for the production of high-value unsaturated single cell oil (SCO) using oleaginous fungi, Mucor circinelloides and Mortierella alpina. Both strains showed good growth and lipid accumulation when using animal fat as a single carbon source. The biomass concentration of 16.7 ± 2.2 gDCW/L and lipid content of 54.1%wt (of dry cell weight) were obtained for Mucor circinelloides in shake flask experiments, surpassing the biomass yield achieved in batch and fed-batch fermentation. In contrast, Mortierella alpina gave the highest biomass concentration (8.3 ± 0.3 gDCW/L) and lipid content (55.8%wt) in fed-batch fermentation. Fat grown Mortierella alpina was able to produce arachidonic acid (ARA), and the highest ARA content of 23.8%wt (of total lipid weight) was in fed-batch fermentation. Gamma-linolenic acid (GLA) was produced by both fungal strains. At the end of fed-batch fermentation, the GLA yields obtained for Mucor circinelloides and Mortierella alpina were 4.51%wt and 2.77%wt (of total lipid weight), respectively. This study demonstrates the production of unsaturated SCO-rich fungal biomass from animal fat by fermentation.

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