Use of hollow fibre-based liquid–liquid–liquid microextraction and high-performance liquid chromatography–diode array detection for the determination of phenolic acids in fruit juices

Saraji, Mohammad; Mousavi, Fatemeh

doi:10.1016/j.foodchem.2010.06.012

Cited by 51 publications

(20 citation statements)

References 37 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…2, and the values for the six phytohormones were in the range 8.5-30%, which may account for the relatively high variability of the method. The EF values for the six compounds, defined as the ratio of the concentrations of the analyte in the acceptor and donor phases [37], varied from 71-to 243-fold. This suggests that although SD-LLLME is not an exhaustive extraction technique, it can still effectively preconcentrate the target compounds in a short time.…”

Section: Methods Validation and Applicationmentioning

confidence: 99%

Direct analysis in real time mass spectrometry combined with single-drop liquid–liquid–liquid microextraction for the rapid analysis of multiple phytohormones in fruit juice

2012

View full text Add to dashboard Cite

A rapid, simple, and efficient method for the fast determination of multiple phytohormones was developed in this work, based on single-drop liquid-liquid-liquid microextraction (SD-LLLME) combined with direct analysis in real-time mass spectrometry (DART-MS). Six phytohormones--indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), jasmonic acid (JA), salicylic acid (SA), abscisic acid (ABA), and gibberellin A(3) (GA(3))--were analyzed simultaneously using this method, and the conditions employed for DART-MS and SD-LLLME were optimized systematically. Satisfactory results were obtained in terms of linearity (R (2) values for all phytohormones were 0.991-0.996), sensitivity (limits of detection were 0.65-72 ng/mL), and repeatability (RSD values were 6.9-14%). In addition, the proposed method was applied to determine the endogenous phytohormones in three kinds of fruit juice. Different concentrations of phytohormones were detected with satisfactory recoveries, and the whole analytical procedure took no more than 30 min. Therefore, this combination of SD-LLLME and DART-MS was shown to be a suitable and effective approach for the fast analysis of targets present at trace level concentrations in complex matrices.

show abstract

Section: Methods Validation and Applicationmentioning

confidence: 99%

Direct analysis in real time mass spectrometry combined with single-drop liquid–liquid–liquid microextraction for the rapid analysis of multiple phytohormones in fruit juice

2012

View full text Add to dashboard Cite

show abstract

“…Plants contain a wide variety of phenolic compounds depending on the species (Bravo, 1998;Saraji & Mousavi, 2010). In general, plants of the Solanaceae family provide both chlorogenic acid and other hydroxycinnamic acids.…”

Section: Food Sources Of Flavonoids and Phenolic Acidsmentioning

confidence: 99%

Antioxidant and Pro-Oxidant Effects of Polyphenolic Compounds and Structure-Activity Relationship Evidence

Yordi¹,

Pérez²,

Matos³

et al. 2012

Nutrition, Well-Being and Health

View full text Add to dashboard Cite

“…In our study, the concentration levels of the target phenolic acids in fruit juice samples varied from 1.0 to 3.2 μg mL −1 . Amounts of target phenolic acids are same and, in some cases, are lower or higher than described elsewhere (Saraji and Mousavi 2010;Schieber et al 2001;Shui and Leong 2002). This may be due to differences in the raw material used, i.e., different fruit cultivars, or to the analytical methodology applied.…”

Section: Simultaneous Quantification Of Phba Va and Ca In Real Samplesmentioning

confidence: 75%

UV–Visible Spectrometry and Multivariate Calibration as a Rapid and Reliable Tool for Simultaneous Quantification of Ternary Mixture of Phenolic Acids in Fruit Juice Samples

2015

View full text Add to dashboard Cite

Phenolic compounds are biologically important molecules existed in many different plants and fruits. There is a need for a reliable analytical method possessing speedy monitoring, ease of operation, and simple instrumentation. We have developed a fast and reliable spectrophotometric method for simultaneous determination of p-hydroxybenzoic (PHBA), vanillic (VA), and caffeic (CA) acids in fruit juice samples. To overcome the severe spectral overlapping, partial least squares (PLS) regression as a multivariate calibration method was successfully developed for the simultaneous determination of PHBA, VA, and CA in ternary solutions. The experimental calibration matrix was designed with 25 ternary mixtures of these compounds, and the calibration models were validated with six synthetic mixtures. Calibration graphs were linear in the range of 0.0-6.0, 0.0-12.0, and 0.0-12.0 μg mL −1 ; limit of detections (LODs) were found to be 0.092, 0.1 l7, and 0.107 μg mL −1 for PHBA, VA, and CA, respectively. The root-mean-square errors of prediction (RMSEPs) for the same order of target compounds were 0.084, 0.146, and 0.114. The accuracy of the method was confirmed with the recoveries ranging between 84 and 107 %. The relative standard deviations (RSDs) for the analytes in the analysis of fruit juice samples were lower than 4 %. The PLS results were found to be in good agreement with those obtained by high-performance liquid chromatographyultraviolet detection (HPLC-UV) method. Such a chemometrics-based protocol may be a promising tool for more analytical applications in real sample monitoring, due to its advantages of simplicity, rapidity, and accuracy.

show abstract

Use of hollow fibre-based liquid–liquid–liquid microextraction and high-performance liquid chromatography–diode array detection for the determination of phenolic acids in fruit juices

Cited by 51 publications

References 37 publications

Direct analysis in real time mass spectrometry combined with single-drop liquid–liquid–liquid microextraction for the rapid analysis of multiple phytohormones in fruit juice

Direct analysis in real time mass spectrometry combined with single-drop liquid–liquid–liquid microextraction for the rapid analysis of multiple phytohormones in fruit juice

Antioxidant and Pro-Oxidant Effects of Polyphenolic Compounds and Structure-Activity Relationship Evidence

UV–Visible Spectrometry and Multivariate Calibration as a Rapid and Reliable Tool for Simultaneous Quantification of Ternary Mixture of Phenolic Acids in Fruit Juice Samples

Contact Info

Product

Resources

About