Use of
            <i>Bifidobacterium dentium</i>
            as an Indicator of the Origin of Fecal Water Pollution

Nebra, Yolanda; Bonjoch, Xavier; Blanch, Anicet R.

doi:10.1128/aem.69.5.2651-2656.2003

Cited by 60 publications

(42 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Consequently, the specific association of B. adolescentis and B. dentium with human sewage could be used to identify the origin of fecal pollution in water. This result agrees with independent studies by different authors who proposed B. adolescentis (17) and B. dentium (23) as human-specific species. Both studies suggested these species for distinguishing human fecal pollution, although some false positives were observed in certain cases (17).…”

supporting

confidence: 83%

“…Additionally, a culture-based method may be limited by the anaerobic physiology of the bacteria. The use of molecular rather than culture-based detection methods could overcome the problems associated with growing strict anaerobes (23). In order to confirm the exclusive presence of human-related Bifidobacterium spp.…”

mentioning

confidence: 99%

“…The incubation period was between 48 and 72 h, as previously described (23). DNA extraction from bifidobacteria and environmental samples was performed as previously described (23). Sewage samples were centrifuged at 250 ϫ g for 15 min to remove major particulate debris.…”

mentioning

confidence: 99%

“…Bifidobacterium strains were grown anaerobically at 37°C on Columbia blood agar (Difco, Detroit, Mich.) supplemented with 5 g of glucose/ liter and 5 g of L-cysteine-HCl/liter before sterilization and reinforced clostridial medium (Oxoid, Basingstoke, Hampshire, England). The incubation period was between 48 and 72 h, as previously described (23). DNA extraction from bifidobacteria and environmental samples was performed as previously described (23).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Multiplex PCR with 16S rRNA Gene-Targeted Primers of Bifidobacterium spp. To Identify Sources of Fecal Pollution

Bonjoch

Blanch

2004

Appl Environ Microbiol

View full text Add to dashboard Cite

Bifidobacteria are one of the most common bacterial types found in the intestines of humans and other animals and may be used as indicators of human fecal pollution. The presence of nine human-related Bifidobacterium species was analyzed in human and animal wastewater samples of different origins by using species-specific primers based on 16S rRNA sequences. Only B. adolescentis and B. dentium were found exclusively in human sewage. A multiplex PCR approach with strain-specific primers was developed. The method showed a sensitivity threshold of 10 cells/ml. This new molecular method could provide useful information for the characterization of fecal pollution sources.

show abstract

supporting

confidence: 83%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Multiplex PCR with 16S rRNA Gene-Targeted Primers of Bifidobacterium spp. To Identify Sources of Fecal Pollution

Bonjoch

Blanch

2004

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…The PCR method performed from BHMup enrichment medium does not present this disadvantage and can be used effectively to detect bifidobacteria as fecal indicators in raw milk cheese industries, instead of or with E. coli. Lynch et al(2002), Nebra et al (2003) and Bonjoch et al (2004) proposed the species B. adolescentis or B. dentium as indicators of fecal pollution. As these species are dominant in human feces, they will indicate a contamination of human origin.…”

Section: Resultsmentioning

confidence: 99%

A PCR method for detection of bifidobacteria in raw milk and raw milk cheese: comparison with culture-based methods

Delcenserie

Bechoux

China

et al. 2005

Journal of Microbiological Methods

View full text Add to dashboard Cite

Bifidobacteria are well known for their beneficial effects on health and are used as probiotics in food and pharmaceutical products. As they form one of the most important groups in both human and animal feces, their use as fecal indicator organisms in raw milk products has recently been proposed. Bifidobacteria species isolated in humans are different from those isolated in animals. It should therefore be possible to determine contamination origin (human or animal).A method of detecting the Bifidobacterium genus was developed by PCR targeting the hsp60 gene. The genus Bifidobacterium was identified by PCR amplification of a 217-bp hsp60 gene fragment. The degenerated primer pair specific to the Bifidobacterium genus used was tested for it specificity on 127 strains. Sensitivity was measured on artificially contaminated samples. Food can however be a difficult matrix for PCR testing since it contains PCR inhibitors. So an internal PCR control was used. An artificially created DNA fragment of 315 bp was constructed. The PCR detection method was tested on raw milk and cheese samples and compared with three culture-based methods, which comprised enrichment and isolation steps. The enrichment step used Brain Heart Infusion medium with propionic acid, iron citrate, yeast extract, supplemented with mupirocin (BHMup) or not (BH) and the isolation step used Columbia blood agar medium, supplemented with mupirocin (CMup) or not (C). The method using mupirocin at both enrichment and isolation steps and the PCR method performed from the culture in BHMup enrichment medium were shown to be the most efficient. No significant difference was observed in raw milk samples between PCR from BHMup and the culture-based method BHMup/CMup, while a significant difference was noticed between the same methods in raw milk cheese samples, which would favor using PCR.The results suggested that PCR on the hsp60 gene was convenient for a rapid detection of bifidobacteria in raw milk and raw milk cheese samples and that bifidobacteria always present throughout raw milk cheese production could be efficiently used as fecal indicators.

show abstract

Detection of Microbial Water Quality Indicators and Fecal Waterborne Pathogens in Environmental Waters: A Review of Methods, Applications, and Limitations

Domingo

Rice

2009

Encyclopedia of Analytical Chemistry

View full text Add to dashboard Cite

Environmental waters are important reservoirs of pathogenic microorganisms, many of which are of fecal origin. In most cases, the presence of pathogens is determined using surrogate bacterial indicators. In other cases, direct detection of the pathogen in question is required. Microbiological methods to determine the presence of indicator organisms or pathogens in environmental samples vary depending on the target organism, sample type, need for quantification, speed of detection, sensitivity, and detection limits. Classical methods depend on cultivation steps while emerging techniques rely on detecting genetic signals from nucleic acid extracted from water samples. Regardless of the method, accurate detection is important to public health officials and monitoring agencies. In this article, we review some of the methods used to detect and quantify indicators of microbial water quality and human fecal pathogens as well as other issues relevant to water fecal pollution.

show abstract

Use of Bifidobacterium dentium as an Indicator of the Origin of Fecal Water Pollution

Cited by 60 publications

References 30 publications

Multiplex PCR with 16S rRNA Gene-Targeted Primers of Bifidobacterium spp. To Identify Sources of Fecal Pollution

Multiplex PCR with 16S rRNA Gene-Targeted Primers of Bifidobacterium spp. To Identify Sources of Fecal Pollution

A PCR method for detection of bifidobacteria in raw milk and raw milk cheese: comparison with culture-based methods

Detection of Microbial Water Quality Indicators and Fecal Waterborne Pathogens in Environmental Waters: A Review of Methods, Applications, and Limitations

Contact Info

Product

Resources

About