2010
DOI: 10.1128/aem.01754-09
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Use of Ichip for High-Throughput In Situ Cultivation of “Uncultivable” Microbial Species

Abstract: One of the oldest unresolved microbiological phenomena is why only a small fraction of the diverse microbiological population grows on artificial media. The "uncultivable" microbial majority arguably represents our planet's largest unexplored pool of biological and chemical novelty. Previously we showed that species from this pool could be grown inside diffusion chambers incubated in situ, likely because diffusion provides microorganisms with their naturally occurring growth factors. Here we utilize this appro… Show more

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Cited by 629 publications
(547 citation statements)
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References 38 publications
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“…E. terrae was isolated with the help of a new cultivation technique, the so‐called iChip, which is a multichannel device composed of several hundred miniature diffusion chambers, each inoculated with a single environmental cell. The iChip then is placed back in the natural environment, which leads to a significantly increased colony count that subsequently can efficiently be cultivated under laboratory conditions (Nichols et al ., 2010). …”
Section: Novel Approaches For Drug Discoverymentioning
confidence: 99%
“…E. terrae was isolated with the help of a new cultivation technique, the so‐called iChip, which is a multichannel device composed of several hundred miniature diffusion chambers, each inoculated with a single environmental cell. The iChip then is placed back in the natural environment, which leads to a significantly increased colony count that subsequently can efficiently be cultivated under laboratory conditions (Nichols et al ., 2010). …”
Section: Novel Approaches For Drug Discoverymentioning
confidence: 99%
“…7 This is where the multichannel device, iChip, 8 has made a considerable contribution enabling the identification of molecules like teixobactin, some of which could be active against drug resistant bacteria. However, the iChip device has its limitations.…”
mentioning
confidence: 99%
“…One illustrative example is the development and use of iChip technology (Nichols et al ., 2010) that permits the growth of uncultured microorganisms, which along with directed screenings against bacterial pathogens, have led to the identification of teixobactin, a novel antibiotic produced by the recently isolated Eleftheria terrae bacterium. This molecule binds to a highly conserved motif of lipid II and lipid III, arresting the cell wall synthesis (Ling et al ., 2015).…”
Section: Highlightmentioning
confidence: 99%