Use of IGL–1 Preservation Solution in Liver Transplantation

Wiederkehr, Julio Cesar; Igreja, Mauro; Nogara, Marcelo Augusto Scheidemantel; Gonçalves, Nídia; Montemezzo, G.P.; Wiederkehr, Henrique de Aguiar; Wassen, M.P.; Nobrega, H.A.; Zenatti, K.B.; Mori, Lorenzo; Tudisco, Marie

doi:10.1016/j.transproceed.2014.05.040

Cited by 21 publications

(14 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The resulting total liver cell suspension was further separated into the PF and the NPF by two low-speed centrifugation steps (36 g and 56 g, 10 min each at 4 • C). Both fractions were cryopreserved at a density of 10-20 million viable cells/mL in IGL1-solution (Institut Georges Lopez, Lissieu, France) [21], supplemented with 15% Hibumine (Baxter, Vienna, Austria) and 10% DMSO (WAK-Chemie Medical, Steinbach, Germany). Progressive cooling was performed at 1 • C/min from 0 to −40 • C and at 2 • C/min from −40 to −80 • C in a Nicool Freezal (Air Liquide, Paris, France).…”

Section: Isolation Cryopreservation and Purification Of Human Primarmentioning

confidence: 99%

Human Liver-Derived Extracellular Matrix for the Culture of Distinct Human Primary Liver Cells

Sarika

Payen

Fléron

et al. 2020

Cells

View full text Add to dashboard Cite

The lack of robust methods to preserve, purify and in vitro maintain the phenotype of the human liver’s highly specialized parenchymal and non-parenchymal cell types importantly hampers their exploitation for the development of research and clinical applications. There is in this regard a growing interest in the use of tissue-specific extracellular matrix (ECM) to provide cells with an in vitro environment that more closely resembles that of the native tissue. In the present study, we have developed a method that allows for the isolation and downstream application of the human liver’s main cell types from cryopreserved material. We also isolated and solubilized human liver ECM (HL-ECM), analyzed its peptidomic and proteomic composition by mass spectrometry and evaluated its interest for the culture of distinct primary human liver cells. Our analysis of the HL-ECM revealed proteomic diversity, type 1 collagen abundance and partial loss of integrity following solubilization. Solubilized HL-ECM was evaluated either as a coating or as a medium supplement for the culture of human primary hepatocytes, hepatic stellate cells and liver sinusoidal endothelial cells. Whereas the solubilized HL-ECM was suitable for cell culture, its impact on the phenotype and/or functionality of the human liver cells was limited. Our study provides a first detailed characterization of solubilized HL-ECM and a first report of its influence on the culture of distinct human primary liver cells.

show abstract

Section: Isolation Cryopreservation and Purification Of Human Primarmentioning

confidence: 99%

Human Liver-Derived Extracellular Matrix for the Culture of Distinct Human Primary Liver Cells

Sarika

Payen

Fléron

et al. 2020

Cells

View full text Add to dashboard Cite

show abstract

“…PNF rates were reported in 15 studies (Table 2 )[ 8 , 9 , 12 , 13 , 21 , 26 - 35 ]. In four studies, PNF was defined as patient death or retransplantation in the first week.…”

Section: Resultsmentioning

confidence: 99%

“…In either case, we found no difference between UW and the other solutions with regard to the risk of PNF. As regards IGL-1 and HTK, two prospective randomized clinical studies with 356 patients reported identical results[ 8 , 21 ]. Similar outcome was detected in a single-center study with 140 patients that compared IGL-1 and UW solutions[ 37 ].…”

Section: Discussionmentioning

confidence: 95%

See 1 more Smart Citation

Compared efficacy of preservation solutions on the outcome of liver transplantation: Meta-analysis

Szilágyi¹,

Mátrai²,

Hegyi³

et al. 2018

WJG

View full text Add to dashboard Cite

AIMTo compare the effects of the four most commonly used preservation solutions on the outcome of liver transplantations.METHODSA systematic literature search was performed using MEDLINE, Scopus, EMBASE and the Cochrane Library databases up to January 31st, 2017. The inclusion criteria were comparative, randomized controlled trials (RCTs) for deceased donor liver (DDL) allografts with adult and pediatric donors using the gold standard University of Wisconsin (UW) solution or histidine-tryptophan-ketoglutarate (HTK), Celsior (CS) and Institut Georges Lopez (IGL-1) solutions. Fifteen RCTs (1830 livers) were included; the primary outcomes were primary non-function (PNF) and one-year post-transplant graft survival (OGS-1).RESULTSAll trials were homogenous with respect to donor and recipient characteristics. There was no statistical difference in the incidence of PNF with the use of UW, HTK, CS and IGL-1 (RR = 0.02, 95%CI: 0.01-0.03, P = 0.356). Comparing OGS-1 also failed to reveal any difference between UW, HTK, CS and IGL-1 (RR = 0.80, 95%CI: 0.80-0.80, P = 0.369). Two trials demonstrated higher PNF levels for UW in comparison with the HTK group, and individual studies described higher rates of biliary complications where HTK and CS were used compared to the UW and IGL-1 solutions. However, the meta-analysis of the data did not prove a statistically significant difference: the UW, CS, HTK and IGL-1 solutions were associated with nearly equivalent outcomes.CONCLUSIONAlternative solutions for UW yield the same degree of safety and effectiveness for the preservation of DDLs, but further well-designed clinical trials are warranted.

show abstract

“…There were 22 articles included in the systematic review, which were combined into 19 data sets after accounting for overlapping data. RCTs or quasi‐RCTs accounted for 9 data sets, whereas 6 and 4 data sets were from retrospective and prospective cohort studies, respectively . Fourteen articles were eligible for meta‐analyses.…”

Section: Resultsmentioning

confidence: 99%

A systematic review and meta‐analysis of cold in situ perfusion and preservation of the hepatic allograft: Working toward a unified approach

et al. 2017

View full text Add to dashboard Cite

The efficacy of cold in situ perfusion and static storage of the liver is a possible determinant of transplantation outcomes. The aim of this study was to determine whether there is evidence to substantiate a preference for a particular perfusion route (aortic or dual) or perfusion/preservation solution in donation after brain death (DBD) liver transplantation. The Embase, MEDLINE, and Cochrane databases were used (1980‐2017). Random effects modeling was used to estimate effects on transplantation outcomes based on (1) aortic or dual in situ perfusion and (2) the use of University of Wisconsin (UW), histidine tryptophan ketoglutarate (HTK), Celsior, and/or Institut Georges Lopez–1 (IGL‐1) solutions for perfusion/preservation. A total of 22 articles were included (2294 liver transplants). The quality of evidence ranged from very low to moderate Grading of Recommendations, Assessment, Development and Evaluations score. Meta‐analyses were conducted for 14 eligible studies. Although there was no difference in the primary nonfunction (PNF) rate, a higher peak alanine aminotransferase (ALT) was recorded in dual compared with aortic‐only UW‐perfused livers (standardized mean difference, 0.24; 95% confidence interval, 0.01‐0.47); a back‐table portal venous flush was undertaken in the majority of aortic‐only perfused livers. There were no relevant differences in peak enzymes, PNF, thrombotic graft loss, biliary complications, or 1‐year graft survival in comparisons between dual‐perfused livers using UW, HTK, Celsior, or IGL‐1. In conclusion, there is no significant evidence that aortic‐only perfusion of the DBD liver compromises transplantation outcomes, and it may be favored because of its simplicity. However, there is currently insufficient evidence to advocate for the use of any particular perfusion/preservation fluid over the others. Liver Transplantation 23 1615–1627 2017 AASLD.

show abstract

Use of IGL–1 Preservation Solution in Liver Transplantation

Cited by 21 publications

References 4 publications

Human Liver-Derived Extracellular Matrix for the Culture of Distinct Human Primary Liver Cells

Human Liver-Derived Extracellular Matrix for the Culture of Distinct Human Primary Liver Cells

Compared efficacy of preservation solutions on the outcome of liver transplantation: Meta-analysis

A systematic review and meta‐analysis of cold in situ perfusion and preservation of the hepatic allograft: Working toward a unified approach

Contact Info

Product

Resources

About