Use of immobilized 3-phytase and flow injection for the determination of phosphorus species in natural waters

McKelvie, Ian D.; Hart, Barry T.; Cardwell, Terence J.; Cattrall, Robert W.

doi:10.1016/0003-2670(95)00373-8

Cited by 36 publications

(31 citation statements)

References 21 publications

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“…They also reported significant phosphatase activity. Exocellular alkaline phosphatase is important for algae and bacteria which use dissolved inorganic phosphorus (McKelvie et al, 1995). Its activity is increased when inorganic phosphorus is low or undetectable (Solarzano, 1978).…”

Section: Discussionmentioning

confidence: 99%

Seasonal evolution of the nutrient pattern on Biscay Bay continental shelf over the years 1999-2000

Loyer¹,

Lampert²,

Ménesguen³

et al. 2006

Sci. Mar.

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Section: Discussionmentioning

confidence: 99%

Seasonal evolution of the nutrient pattern on Biscay Bay continental shelf over the years 1999-2000

Loyer¹,

Lampert²,

Ménesguen³

et al. 2006

Sci. Mar.

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“…[68] Relative to sediments, there have been far fewer studies of inositol phosphates in the water column. Most have used phytase to hydrolyse phytate to orthophosphate [69][70][71] ; although, as pointed out by several authors, phytase is not absolutely specific for inositol phosphates but can also hydrolyse other organic P esters. [64,71] Inositol phosphates have also been detected in NaOH-EDTA extracts of freeze-dried water samples using 31 P NMR spectroscopy [72] although, as will be discussed later, this technique is probably not applicable for routine analysis.…”

Section: Identificationmentioning

confidence: 99%

“…The enzyme may hydrolyse compounds other than the target molecule as well Doesn't require specialist training May require construction of specific pieces of equipment (e.g. immobilised enzyme columns) [70] High sample throughput P NMR spectroscopy Canvases all classes of organic P species in a sample…”

Section: Synthesismentioning

confidence: 99%

Organic phosphorus in the aquatic environment

Baldwin¹

2013

Environ. Chem.

104

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Environmental context. Organic phosphorus can be one of the major fractions of phosphorus in many aquatic ecosystems. This paper discusses the distribution, cycling and ecological significance of five major classes of organic P in the aquatic environment and discusses several principles to guide organic P research into the future.Abstract. Organic phosphorus can be one of the major fractions of phosphorus in many aquatic ecosystems. Unfortunately, in many studies the 'organic' P fraction is operationally defined. However, there are an increasing number of studies where the organic P species have been structurally characterised -in part because of the adoption of 31 P NMR spectroscopic techniques. There are five classes of organic P species that have been specifically identified in the aquatic environment -nucleic acids, other nucleotides, inositol phosphates, phospholipids and phosphonates. This paper explores the identification, quantification, biogeochemical cycling and ecological significance of these organic P compounds. Based on this analysis, the paper then identifies a number of principles which could guide the research of organic P into the future. There is an ongoing need to develop methods for quickly and accurately identifying and quantifying organic P species in the environment. The types of ecosystems in which organic P dynamics are studied needs to be expanded; flowing waters, floodplains and small wetlands are currently all under-represented in the literature. While enzymatic hydrolysis is an important transformation pathway for the breakdown of organic P, more effort needs to be directed towards studying other potential transformation pathways. Similarly effort should be directed to estimating the rates of transformations, not simply reporting on the concentrations. And finally, further work is needed in elucidating other roles of organic P in the environment other than simply a source of P to aquatic organisms.

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“…wheat, first acts on the C 6 atom while that from microbial sources acts on the C 3 atom. McKelvie et al [59,152] used a FI system with immobilised phytase for the determination of phytic acid in soils. Adenosine-5 -triphosphate was also hydrolysed but in low yields compared with phytic acid.…”

Section: Enzymatic Degradationmentioning

confidence: 99%

“…Adenosine-5 -triphosphate was also hydrolysed but in low yields compared with phytic acid. Phytase has also been applied to the determination of phytic acid in the marine environment, but with low recoveries [152].…”

Section: Enzymatic Degradationmentioning

confidence: 99%

Sampling, sample treatment and quality assurance issues for the determination of phosphorus species in natural waters and soils

Worsfold

Gimbert

Mankasingh

et al. 2005

Talanta

162

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Use of immobilized 3-phytase and flow injection for the determination of phosphorus species in natural waters

Cited by 36 publications

References 21 publications

Seasonal evolution of the nutrient pattern on Biscay Bay continental shelf over the years 1999-2000

Seasonal evolution of the nutrient pattern on Biscay Bay continental shelf over the years 1999-2000

Organic phosphorus in the aquatic environment

Sampling, sample treatment and quality assurance issues for the determination of phosphorus species in natural waters and soils

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