Use of Immobilized Pseudomonas sp. as Whole Cell Catalyst for the Transesterification of Used Cotton Seed Oil

Ali, Amjad; Kaur, Mandeep; Mehra, Upasna

doi:10.5650/jos.60.7

Cited by 20 publications

(5 citation statements)

References 23 publications

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“…3.1.1.3 , for the transesterification of triglycerides. Direct application of lipase producing microorganisms as whole cell , pure lipase and immobilized lipase has been well documented in literature for the transesterification of a variety of triglycerides 6,7 . However, the biodiesel production by enzymatic method has not been adopted industrially as enzyme catalyzed reactions are relatively slow 8 and often not lead to the completion of the reaction 96.5 FAMEs yield .…”

Section: Introductionmentioning

confidence: 99%

Effect of Metal Ions on the Hydrolytic and Transesterification Activities of Candida rugosa Lipase

Katiyar

Ali

2013

J. Oleo Sci.

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Section: Introductionmentioning

confidence: 99%

Effect of Metal Ions on the Hydrolytic and Transesterification Activities of Candida rugosa Lipase

Katiyar

Ali

2013

J. Oleo Sci.

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“…A yield of 71% was obtained after 165 h at 37 °C with step‐wise addition of methanol 72 . Beside Rhizopus oryzae , other whole cell biocatalysts such as Aspergillus niger , 73,74 Pseudomonas fluorescens , 75 and Pseudomonas sp 76 . have also been studied, which efficiently catalyzed the methanolysis reaction for biodiesel production.…”

Section: Lipase‐mediated Biodiesel Productionmentioning

confidence: 99%

Biodiesel production with enzymatic technology: progress and perspectives

Pasha

Dai

Liu

et al. 2021

Biofuels Bioprod Bioref

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Rapid changes in the global climate resulting from anthropogenic activities and greenhouse gas emissions from fossil resources are encouraging commercial and academic research to seek out new routes for the production of sustainable fuels. Biodiesel, as a renewable fuel, has long been recognized as one of the solutions to meet energy demand in a climate‐constrained world. Although, several routes have been devised for biodiesel production, the enzymatic route has attracted substantial research interest. Here we discuss enzymatic esterification / transesterification technology and its contribution to the green synthesis of biodiesel. This review provides a comprehensive assessment of the fundamentals of enzymatic reaction, such as the impact of different reaction components (lipase, acyl acceptor, substrate, and reaction media) on the process parameters, along with recent developments in improving enzymatic biodiesel production, such as different lipases, combinations of lipases, and two‐step methods. Kinetics and the mechanism of enzymatic reaction when lipase is used as a catalyst are also explained in greater detail. Finally, the opportunities and challenges for the development of potentially sustainable and eco‐friendly enzymatic biodiesel technology are discussed. © 2021 Society of Industrial Chemistry and John Wiley & Sons Ltd.

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“…Several methods have been reported 21) including the immobilization of whole cell 22) or lipase on solid surfaces 21) , to overcome the problems associated with the application of JSM 6510LV, FTIR spectra were recorded on Thermo Scientific Nicolet iS10 FT-IR spectrometer, FT-NMR spectra were recorded on a Bruker Avance-II (400 MHz) spectrophotometer and absorbance spectra were recorded on Perkin Elmer (Lambda-35) UV-Visible spectrophotometer.…”

Section: -7)mentioning

confidence: 99%

Immobilization of Candida rugosa Lipase on MCM-41 for the Transesterification of Cotton Seed Oil

Katiyar

Ali

2012

J. Oleo Sci.

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pure lipase. In literature a variety of carriers, e.g., acrylic resin, textile membrane, polypropylene, celite, mesoporous silica, and diatomaceous earth, have been employed for the lipase immobilization by physical adsorption technique 18). Silica-based porous material, because of their high surface area and tunable pore diameter, also gained popularity in recent past 23) as support for the immobilization of large molecules viz., enzymes. In continuation to our earlier efforts 10, 11, 22) , in present study Candida rugosa lipase (CRL) has been immobilized on MCM-41 by physical adsorption method, and resulted solid biocatalyst (CRL-MCM-41) has been employed for the transesterification of cotton seed oil with methanol. 2 Materials and methods 2.1 Enzyme solutions and instrumentations The solutions of CRL (Sigma-Aldrich) of 3.4 mg/ml concentration were prepared by shaking 68 mg of enzyme in 20 mL buffer solution (25 mM) of desired pH (3-10) at 25℃. Acetate, phosphate and tris/HCl were used for making the buffer solutions of pH 3-5, 6-8, and 9-10, respectively. The CRL solutions thus obtained were centrifuged and clear supernatant was collected and stored at 4℃ for further use. Powder X-ray diffraction (XRD) data has been collected on Panalytical' s X' Pert Pro with Cu Kα radiation, scanning electron microscopy (SEM) images were recorded on JEOL

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Use of Immobilized Pseudomonas sp. as Whole Cell Catalyst for the Transesterification of Used Cotton Seed Oil

Cited by 20 publications

References 23 publications

Effect of Metal Ions on the Hydrolytic and Transesterification Activities of Candida rugosa Lipase

Effect of Metal Ions on the Hydrolytic and Transesterification Activities of Candida rugosa Lipase

Biodiesel production with enzymatic technology: progress and perspectives

Immobilization of Candida rugosa Lipase on MCM-41 for the Transesterification of Cotton Seed Oil

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