Use of immuno-electro-diffusion on cellulose acetate for the research of the precipitating antibodies in the screening of patients suspected of farmer's lung disease

Gari, M.; Smets, P.; Pinon, J. M.; Recco, P.; Séguéla, J. P.

doi:10.1007/bf00466069

Cited by 12 publications

(7 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ELIFA method developed in our laboratory involves two steps. Firstly, coimmunoelectrodiffusion (CO-IF,D) was carried out on cellulose acetate [8] running three serum samples simultaneously in parallel on the same membrane in Tris glycine buffer (pH 9; 0 05 M). Fifteen /d of undiluted serum as a drop was placed on the anode side and 15 /d of antigen, at a concentration of 2 ^g///l.…”

Section: / Enzyme-linked Immuno-fittration-assciv ! Elifa)mentioning

confidence: 99%

“…The most comtnonly used test is the precipitin test, performed either by Ouchterlony double diffusion or by gel immuno-electrophoresis [7]. Recently the immuno-electro-diffusion test (IED) on cellulose acetate has tended to replace the last two methods because it is faster and tnore sensitive [8]. The demonstration of precipitins in serum only shows that there has been exposure to the antigen as they are found both in patients and asymptomatic farmers [9.10].…”

mentioning

confidence: 99%

See 1 more Smart Citation

Enzyme‐linked immunofiltration assay (ELIFA) for the detection of specific antibodies (IgG‐IgM‐IgA‐IgE) in farmer's lung disease

Gari

Pinon

Poirriez

et al. 1986

Clin Experimental Allergy

Self Cite

View full text Add to dashboard Cite

Abstract. Until now the detection of chymotrypsic or polysaccharidic arcs among precipitating systems indicated a positive diagnosis of Farmer's lung disease (FLD). It was shown that the occurrence of chymotrypsic activity is not absolutely correlated with the presence of clinical disease. The presence of different classes of specific precipitating antibodies was looked for and correlated with clinical symptoms in an attempt to find additional positive criteria for the diagnosis of FLD. The enzyme‐linked immunofiltration assay (ELIFA) was adapted to detect the different specific antibody classes. 1400 serum samples were studied from 1350 farmers exposed to mouldy hay. Of 1358 samples examined from asymptomatic subjects tested as part of a systematic screening of the profession. 1356 had either no precipitins or have precipitins detected solely against non‐chymotrypsic and non‐polysaccharidic antigens. Ten specimens with precipitins joined the study. Only two serum samples from two symptomless individuals showed positive serological criteria for FLD as evidenced by the presence of a chymotrypsic band. The remaining forty‐two serum samples from twenty‐four patients with clinical signs of extrinsic allergic alveolitis had serological criteria for FLD as either chymotrypsic or polysaccharidic arcs. Only specific IgG in asymptomatic subjects was demonstrated, and when a supplementary class of specific antibody (IgM, IgA or IgE) was detected, there was clinical evidence of FLD in all except one case. Thus ELIFA could prove to be a useful tool for the positive diagnosis and prognosis of patients with FLD. In addition this method allows the recognition of a particular functional antigenic fraction of M. faeni by all classes of specific antibodies between different serum samples.

show abstract

Section: / Enzyme-linked Immuno-fittration-assciv ! Elifa)mentioning

confidence: 99%

mentioning

confidence: 99%

Enzyme‐linked immunofiltration assay (ELIFA) for the detection of specific antibodies (IgG‐IgM‐IgA‐IgE) in farmer's lung disease

Gari

Pinon

Poirriez

et al. 1986

Clin Experimental Allergy

Self Cite

View full text Add to dashboard Cite

show abstract

“…These percentages were higher than in farmers, where about 9% of subjects had precipitin lines for Thermopolyspora faena [4], even when results from immunoelectrophoresis, usually used in farmer's lung studies, were compared [6]. Since only 20.5% of workers asked to participate were included, we cannot exclude that symptomatic workers agreed to participate more often than healthy workers, and that prevalence of sensitization was lower in the whole population than in the group of responders.…”

Section: Discussionmentioning

confidence: 76%

“…Carbon monoxide (CO) transfer capacity (TCO) was measured by the single-breath technique in 66 (54%) workers, using a Hypercompact (Fukuda) spirometer. Blind to clinical, functional, and radiological ®ndings, immunoelectrophoresis and electrosyneresis (or immunoelectrodiusion on cellulose acetate) [6] were carried out to quantify precipitation lines of sausage mould extract, P. nalgiovense and P. chrysogenum, in the blood serum of 91 subjects and in 16 blood-donor controls. Sausage mould extracts and Penicillium extracts were prepared according to the method described by Pepys et al [7].…”

Section: Methodsmentioning

confidence: 99%

Symptoms and serum precipitins in workers exposed to dry sausage mould: consequences of exposure to sausage mould

Rouzaud¹,

Soulat²,

Trela³

et al. 2001

International Archives of Occupational and Environmental Health

Self Cite

View full text Add to dashboard Cite

show abstract

“…The presence of precipitating antibodies against offending antigens has often been considered as simply a marker of exposure (American Thoracic Society, 1998;Cormier et al, 2004;Khan et al, 1995); however, Lacasse et al (2003) reported that such antibodies are significant predictive factors of hypersensitivity pneumonitis [odds ratio (OR): 5.3; 95 % confidence interval (CI): 2.7-10.4] (Lacasse et al, 2003). Several techniques are commonly used in the laboratory for the detection of precipitins or IgG antibodies: immunodiffusion, electrosyneresis on cellulose acetate (Gari et al, 1982) and ELISA (Fenoglio et al, 2007;Reboux et al, 2007). We previously showed that the detection of L. corymbifera antigen by electrosyneresis discriminated FLD patients from healthy exposed farmers with a sensitivity of 87 % and a specificity of 100 % (Reboux et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Western blotting as a tool for the serodiagnosis of farmer’s lung disease: validation with Lichtheimia corymbifera protein extracts

Rognon

Reboux

Roussel

et al. 2015

Journal of Medical Microbiology

View full text Add to dashboard Cite

Electrosyneresis and double diffusion are immunoprecipitation techniques commonly used in the serological diagnosis of Farmer's lung disease (FLD). These techniques are reliable but lack standardization. The aim of this study was to evaluate Western blotting for the serodiagnosis of FLD. We carried out Western blotting with an antigenic extract of Lichtheimia corymbifera, an important aetiological agent of the disease. The membranes were probed with sera from 21 patients with FLD and 21 healthy exposed controls to examine the IgG antibody responses against purified somatic antigens. Given the low prevalence of the disease, 21 patients could be considered as a relevant series. Four bands were significantly more frequently represented in membranes probed with FLD sera (bands at 27.7, 40.5, 44.0 and 50.5 kDa) than those probed with control sera. We assessed the diagnostic value of different criteria alone or in combination. The diagnostic accuracy of the test was highest with the inclusion of at least two of the following criteria: at least five bands on the strip and the presence of one band at 40.5 or 44.0 kDa. Sensitivity, specificity and positive and negative predictive values were all 81 %, and the odds ratio was 18.06. Inclusion of bands of high intensity diminished rather than improved the diagnostic value of the test. We concluded that Western blotting is a valuable technique for the serodiagnosis of FLD. The industrial production of ready-to-use membranes would enable the routine use of this technique in laboratories, and provide reliable and standardized diagnostic results within a few hours.

show abstract

Use of immuno-electro-diffusion on cellulose acetate for the research of the precipitating antibodies in the screening of patients suspected of farmer's lung disease

Cited by 12 publications

References 4 publications

Enzyme‐linked immunofiltration assay (ELIFA) for the detection of specific antibodies (IgG‐IgM‐IgA‐IgE) in farmer's lung disease

Enzyme‐linked immunofiltration assay (ELIFA) for the detection of specific antibodies (IgG‐IgM‐IgA‐IgE) in farmer's lung disease

Symptoms and serum precipitins in workers exposed to dry sausage mould: consequences of exposure to sausage mould

Western blotting as a tool for the serodiagnosis of farmer’s lung disease: validation with Lichtheimia corymbifera protein extracts

Contact Info

Product

Resources

About