Use of in vitro assays to assess the potential antigenotoxic and cytotoxic effects of saffron (Crocus sativus L.)

Abdullaev, Fikrat I.; Riverón-Negrete, Leticia; Caballero-Ortega, Heriberto; Hernández, Javier; Pérez-López, Israel; Pereda‐Miranda, Rogelio; Espinosa-Aguirre, J.J.

doi:10.1016/s0887-2333(03)00098-5

Cited by 130 publications

(89 citation statements)

References 21 publications

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“…Regarding saffron's anti oxidants' effects in steroid hormones biosynthesis it seems that saffron can affect the male sexual hormones concentration. The achieved results on low dosages of saffron in this research also confirm the saffron's fame in traditional medicine as an enhancing drug about sexual power (Abdullaev et al, 2003). On the other hand, this study's results revealed that 200 mg kg −1 dosage of saffron has had inhibitory and toxic effects on testicle' tissue and spermatogenesis.…”

Section: Resultssupporting

confidence: 78%

“…Saffron through enhancing the defensive anti-oxidant system not only reduces the oxidative stresses but also increases the life time and numbers of spermatosoids (Cao et al, 2004;Ochiai et al, 2004). The conclusions of this research are also confirming saffron's popularity in traditional medicine as an enhancing the sexual power drug (Abdullaev et al, 2003). According to these results, saffron's extract can modify reproductive activities in male laboratory mice.…”

Section: Resultssupporting

confidence: 67%

“…Considering contradictory reports about toxicity of saffron, there has been confusion on applying it as a herbal medicine. Some reports suggest that saffron is fully nontoxic herb (Abdullaev et al, 2003;Abdullaev and Espinosa-Aguirre, 2004). Contrasting this it has been specified that krustyn has some teratogenic impacts (Martin et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Effect of Saffron on Histomorphometric Changes of Testicular Tissue in Rat

2011

American Journal of Animal and Veterinary Sciences

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Problem statement: Saffron is widely used as a food flavor and has well known medicinal effects. This drug has an effect on steroid and sexual hormones. The aim of this study was investigation of Saffron orally administration on histomorphometric change of testicular tissue in rat. In this experimental study, 40 male rats are used (4 groups and 10 rats in each group). Approach: Animals were received Saffron (50, 100 and 200 mg kg −1 ) daily orally administration, during 28 days. As one group was control (normal saline) and three groups was drug consumer. The testis tissue was sampled after passing of above time and section providing, was stained by H and E. Results: The histomorphometric changes of testicular tissue which include, seminiferous tubules diameter, seminiferous epithelial thickness, interstitial tissue and testicular capsule thickness were studied. Results showed that seminiferous tubules diameter, seminiferous epithelial thickness had significantly decreased (p<0.05) but interstitial tissue thickness had significantly increased (p<0.05) compared with control group. Testicular capsule thickness had not significantly changed. Conclusion: Our results showed that seminiferous tubules diameter, seminiferous epithelial thickness and interstitial tissue thickness in rat testis were significantly changed after Saffron orally administration. This results probably via the reducing of serum testosterone concentration. Effect of Saffron on spermatogenesis index and unfertilization in human needed to be more investigation.

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Section: Resultssupporting

confidence: 78%

Section: Resultssupporting

confidence: 67%

Section: Introductionmentioning

confidence: 99%

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Effect of Saffron on Histomorphometric Changes of Testicular Tissue in Rat

2011

American Journal of Animal and Veterinary Sciences

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“…It has been reported that saffron or its ingredients such as crocin and dimethylcrocin are not mutagenic or genotoxic (Salomi et al, 1991a;Abdullaev et al, 2002Abdullaev et al, , 2003. Premkumar et al also showed that saffron aqueous extract protects from genotoxicity as well as genotoxins-induced oxidative stress in mice (Premkumar et al, 2001(Premkumar et al, , 2003(Premkumar et al, , 2006.…”

Section: Effect Of Saffron and Crocin On Mms-induced Dna Damagementioning

confidence: 99%

Protective Effect of Crocus sativus Stigma Extract and Crocin (trans-crocin 4) on Methyl Methanesulfonate–Induced DNA Damage in Mice Organs

Hosseinzadeh

Abootorabi

Sadeghnia

2008

DNA and Cell Biology

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This study was designed to examine the effect of aqueous extract of Crocus sativus stigmas (CSE) and crocin (trans-crocin 4) on methyl methanesulfonate (MMS)-induced DNA damage in multiple mice organs using comet assay. Adult male NMRI mice in different groups were treated with either physiological saline (10 mL=Kg, intraperitoneal [ip]), CSE (80 mg=Kg, ip), crocin (400 mg=Kg, ip), MMS (120 mg=Kg, ip), and CSE (5, 20, and 80 mg=Kg, ip) 45 min prior to MMS administration or crocin (50, 200, and 400 mg=Kg, ip) 45 min prior to MMS administration. Mice were scarified about 3 h after each different treatment, and the alkaline comet assay was used to evaluate the effect of these compounds on DNA damage in different mice organs. The percent of DNA in the comet tail (% tail DNA) was measured. A significant increase in the % tail DNA was seen in nuclei of different organs of MMS-treated mice. In control groups, no significant difference was found in the % tail DNA between CSE-or crocin-pretreated and saline-pretreated mice. The MMS-induced DNA damage in CSEpretreated mice (80 mg=Kg) was decreased between 2.67-fold (kidney) and 4.48-fold (lung) compared to those of MMS-treated animals alone ( p < 0.001). This suppression of DNA damage by CSE was found to be depended on the dose, which pretreatment with CSE (5 mg=Kg) only reduced DNA damage by 6.97%, 6.57%, 7.27%, and 9.90% in liver, lung, kidney, and spleen, respectively ( p > 0.05 as compared with MMS-treated group). In the same way, crocin also significantly decreased DNA damage by MMS (between 4.69-fold for liver and 6.55-fold for spleen, 400 mg=Kg), in a dose-dependent manner. These data indicate that there is a genoprotective property in CSE and crocin, as revealed by the comet assay, in vivo.

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“…Saffron with scientific name Crocus sativus L. belongs to the family of the lily and it is one the most valuable and precious farming plant species in the world that are often planted areas with an arid climate (Abdullaev et al, 2003;Melnyk et al, 2010;Molina et al, 2005), yield and quality of saffron are affected by different aspects of economic, social, cultural, educational of saffron planting (Aghaee and Rezagholizadeh, 2011). Better farming and improvement operations are process of production and supplying product and finally creating necessary changes for qualitative and quantitative improvement of product as a result of research, education and promotion of new methods of planting, growing and harvesting product.…”

Section: Introductionmentioning

confidence: 99%

Effect of Nano Iron Chelate Fertilizer on Iron Absorption and Saffron (Crocus sativus L.) Quantitative and Qualitative Characteristics

Farahani¹,

Khalesi²,

Sharghi³

2015

Asian J. of Biological Sciences

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In order to investigate the effect of nano iron chelate and compare to EDDHSA chelate on saffron, a factorial experiment with six treatments and three replications was conducted in saffron research farm of Shahed University in crop year of 2013-2014. Treatments included iron fertilizer at two levels of application of nano iron chelate fertilizer and iron chelate fertilizer with base of EDDHSA and the second agent was amount of fertilizer at three levels of 0,5 and 10 kg haG 1 . Number of flowers, flowers performance, yield of wet and dry stigmas, amount of chlorophyll a and b, total chlorophyll, leaf area index, yield of dry leaf, concentration of leaf iron and total iron were investigated in this study. Qualitative features of Saffron including secondary metabolites of crocin (color agent), picrocrocin (taste agent) and safranal (scent) were measured. The results showed that all traits under study except picrocrocin, safranal, crocin, chlorophyll b, total chlorophyll and leaf area were affected by type, amount or interaction of type in amount of fertilizer. With increase of iron nano fertilizer content by 10 kg of flower number and yield of wet flower increased compared to the control. But application of 5 kg nano chelate led to increased yield of dry stigma, yield of dry leaf, concentration of leaf iron and total iron, compared to control. The overall results showed that a nano-based iron fertilizer is more effective than micro and 5 kg application of this fertilizer is superior to 10 kg.

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Use of in vitro assays to assess the potential antigenotoxic and cytotoxic effects of saffron (Crocus sativus L.)

Cited by 130 publications

References 21 publications

Effect of Saffron on Histomorphometric Changes of Testicular Tissue in Rat

Effect of Saffron on Histomorphometric Changes of Testicular Tissue in Rat

Protective Effect of Crocus sativus Stigma Extract and Crocin (trans-crocin 4) on Methyl Methanesulfonate–Induced DNA Damage in Mice Organs

Effect of Nano Iron Chelate Fertilizer on Iron Absorption and Saffron (Crocus sativus L.) Quantitative and Qualitative Characteristics

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