Use of Inhibitors in Cholinesterase Histochemistry

Pc, Diegenbach

doi:10.1038/207308a0

Cited by 23 publications

(2 citation statements)

References 4 publications

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“…A number of points may be raised which indicate the need for further work from the standpoints of physiology and toxicology. The histochemical tests employed here do not distinguish between AChE and ChE; this requires the use of selective inhibitors, as discussed for example by Pearse ( 1960), and Diegenbach (1965). While the use of whole mounts ensures a minimum loss of enzymes in processing, it does not allow precise localizations of enzymes to be made, as is possible with either thin sections under light microscopy or with the electron microscope.…”

Section: Discussionmentioning

confidence: 99%

INHIBITION OF CHOLINESTERASE IN THE CATTLE GRUB HYPODERMA BOVIS (DIPTERA: OESTRIDAE) AFTER TREATMENT OF THE HOST CATTLE WITH SYSTEMIC INSECTICIDES

Steward¹

1969

Can Entomol

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Cattle grubs, first-instar larvae of Hypoderma bovis (L.), were removed from nine slaughtered heifers. Two of these heifers had previously been treated with coumaphos, three with Ruelene, and two with trichlorfon. The remaining two heifers were not treated with insecticides and served as sources of control grubs. The grubs, after removal from the hosts and fixation in formalin, were incubated with one of the two cholinesterase substrates, acetylthiocholine iodide or 5-bromoindoxyl acetate. Cholinesterase was completely inhibited in the central nervous systems of the grubs whose hosts were treated with Ruelene. This inhibition was also virtually complete in grubs from heifers treated with trichlorfon and was usually complete in those from the heifers treated with coumaphos. This is presented as histochemical evidence that the three systemic insecticides inhibit the cholinergic enzymes in the grubs, thus causing them to die while still in the body of the host.

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Section: Discussionmentioning

confidence: 99%

INHIBITION OF CHOLINESTERASE IN THE CATTLE GRUB HYPODERMA BOVIS (DIPTERA: OESTRIDAE) AFTER TREATMENT OF THE HOST CATTLE WITH SYSTEMIC INSECTICIDES

Steward¹

1969

Can Entomol

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“…After washing the sections, the sites of enzyme activity were visualized by using fresh, dilute ammonium sulphide. Sections were air dried at 37°C and then mounted in Canada Balsam in tctraehlorethylene [371 The differential inhibitors used as a check on the types of cholinesterases present were 1 x 10 5 M physostigmine for both enzymes [9], 1x10 5 M 1,5-bis (4-trimethylammonium phenyl) penlan-3-one di-iodide (Wellcome Laboratories 62C47) to in hibit acetylcholinesterase (15), and 2x10 5 M N, N-di-isopropylphosphorodiamidic fluoride (Mipafox) to inhibit butyrylcholinesterase [211. To prevent artifacts due to dissociation of the enzyme-inhibitor complex all inhibitions were carried out by coincubation.…”

mentioning

confidence: 99%

Esterase Histochemistry of Sebaceous Glands

Ballantyne

Bunch

1967

Dermatology

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Cholinesterase activity in the harderian gland of anas domesticus

Fourman

Ballantyne

1967

Anat. Rec.

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The histochemical localization and quantitative levels of acetylcholinesterase and butyrylcholinesterase have been determined in the Harderian glands of normal ducks and ducks fed with salt to increase their serum sodium concentrations;there was no significant difference between the two groups.Acetylcholinesterase was present at a mean concentration of 68.2 pM/gm/hour (range 65.5 -72.7 pM/gm/hour) in the normal Sniraals and 67.6 pM/gm/hour (range 63.8-76.4pM/gm/hour) in the salt-fed gruup; the enzyme was present in nerves related to blood vessels. Butyrylcholinesterase was present at a mean concentration of 42.5 pM/gm/hour (range 20.4 -58.7 pM/gm/hour) in the control animals and 40.2 pM/gm/hour (range 29.3 -61.3 pM/gm/hour) in the salt-fed group; the enzyme was present in epithelial cells and in nerves related to blood vessels similar to those containing acetylcholinesterase. There was a close correlation between the subjective assessment of cholinesterase activity based on histochemical techniques and the amount quantitatively measured. The gland probably secretes mucus and lipids; the epithelial butyrylcholinesterase may play a role in lipid metabolism in the tissue. The nerves may regulate the flow of blood through the gland.

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Use of Inhibitors in Cholinesterase Histochemistry

Cited by 23 publications

References 4 publications

INHIBITION OF CHOLINESTERASE IN THE CATTLE GRUB HYPODERMA BOVIS (DIPTERA: OESTRIDAE) AFTER TREATMENT OF THE HOST CATTLE WITH SYSTEMIC INSECTICIDES

INHIBITION OF CHOLINESTERASE IN THE CATTLE GRUB HYPODERMA BOVIS (DIPTERA: OESTRIDAE) AFTER TREATMENT OF THE HOST CATTLE WITH SYSTEMIC INSECTICIDES

Esterase Histochemistry of Sebaceous Glands

Cholinesterase activity in the harderian gland of anas domesticus

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