The features of the fatty acid composition of oils and mayonnaise samples prepared on the basis of sunflower, linseed and hemp oils were determined by the method of gas-liquid chromatography. The ratio between the content of PUFAs of the ω-9/ω-6/ω-3 families in the investigated sunflower oil samples was established, which was 177 : 553 : 1. This is caused by the high content of linoleic and oleic acids and the low content of α-linolenic acid (ω-3 ) in sunflower oil. In the studied samples of linseed oil, the content of α-linolenic acid was 52 %, and the content of oleic (ω-9) and linoleic (ω-6) acids was 22.3 and 16.2 %, respectively. Therefore, the ratio between PUFAs ω-9/ω-6/ω-3 in the studied samples of linseed oil was 1.4 : 1 : 3.2. In the tested samples of hemp oil, the content of linoleic acid (ω-6) was 55.7 %, and the content of α-linolenic acid (ω-3) and oleic acid (ω-9) was 18.2 % and 13.2 %, respectively. The ratio between ω-9/ω-6/ω-3 PUFAs in the tested hemp oil samples was 1.4 : 4.5 : 1. Research has established a high content of PUFAs of the ω-6 family and a low content of PUFAs of the ω-3 family in the control sample of mayonnaise (K), which is made using sunflower oil with a content of 71.78 %. This sample was characterized by the ratio of PUFAs of the ω-3, ω-6 and ω-9 families as 1 : 13 : 7.3. In the test sample of mayonnaise (D) containing 23.3 % each of sunflower, linseed and hemp oils, the total content of PUFAs of the ω-3 family was 24.1 %, which is 20.4 % more than in sample K. The higher content of PUFAs of the ω-3 family ω-3 in sample D is caused by the high content of α-linolenic acid in linseed and hemp oils. The total content of PUFAs of the ω-6 family in mayonnaise sample D was 47.4 %, and in sample K – 49.1 %. The content of PUFAs of the ω-9 family in sample D was 18.8 %, and in sample K – 26.9 %. Therefore, the ratio between the content of PUFAs of the ω-3/ω-6/ω-9 families in sample D was 1.3 : 2.5 : 1. The ratio between the content of PUFAs/PUFAs in mayonnaise sample D was 1 : 9.3, while in sample K – 1 : 4.5. Thus, replacing 2/3 of sunflower oil with linseed oil and hemp oil in equal parts in the technological process of making sample D of mayonnaise gives a number of positive advantages in the biological value of the product, compared to sample K, which contains only sunflower oil in its composition. This is a decrease in the content of PUFAs of the ω-6 family, an increase in the content of PUFAs of the ω-3 family, optimization of the balance between the content of PUFAs of the ω-3 / ω-6 / ω-9 families, a decrease in the content of saturated fatty acids and, accordingly, an increase in the content of unsaturated. The results of the analysis of the fatty acid composition of the mayonnaise D sample allow us to recommend this emulsion oil-fat product as a functional product due to the balanced ratio between the content of PUFAs of the ω-3 / ω-6 / ω-9 families.