Use of Inoculator Bacteria to Promote Tuber melanosporum Root Colonization and Growth on Quercus faginea Saplings

Piñuela, Yasmine; Alday, Josu G.; Oliach, Daniel; Bolaño, Francesc; Colinas, Carlos; Bonet, J. A.

doi:10.3390/f11080792

Cited by 9 publications

(4 citation statements)

References 38 publications

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“…roots was observed in the presence of P. fluorescens [ 30 ]. Recently, the efficacy of bacteria in promoting T. melanosporum root colonization was assessed in Quercus faginea [ 31 ]. The authors showed that P. fluorescens increased ECM colonization by about 10% compared to the control (from 26.8 to 35.1%) after one year of co-inoculation with both bacteria and mycorrhizal fungi.…”

Section: Discussionmentioning

confidence: 99%

“…They play a role in influencing the aroma, [ 28 ] promoting plant growth, protecting against pathogens, and nitrogen fixation [ 29 ]. Among cultivation practices, it has been shown that combined inoculation of ECM and plant-growth-promoting rhizobacteria (PGPR) is an effective strategy for improving the quality of seedlings, increasing plant survival, enhancing mycorrhizal symbiosis establishment, and promoting plant growth [ 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Bacteria Inoculation on Colonization of Roots by Tuber melanosporum and Growth of Quercus ilex Seedlings

Giorgi,

Amicucci,

Landi

et al. 2024

Plants

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Tuber melanosporum is an ascomycete that forms ectomycorrhizal (ECM) symbioses with a wide range of host plants, producing edible fruiting bodies with high economic value. The quality of seedlings in the early symbiotic stage is important for successful truffle cultivation. Numerous bacterial species have been reported to take part in the truffle biological cycle and influence the establishment of roots symbiosis in plant hosts and the development of the carpophore. In this work, three different bacteria formulations were co-inoculated in Quercus ilex L. seedlings two months after T. melanosporum inoculation. At four months of bacterial application, the T. melanosporum ECM root tip rate of colonization and bacterial presence were assessed using both morphological and molecular techniques. A 2.5-fold increase in ECM colonization rate was found in the presence of Pseudomonas sp. compared to the seedlings inoculated only with T. melanosporum. The same treatment caused reduced plant growth either for the aerial and root part. Meanwhile, the ECM colonization combined with Bradyrhizobium sp. and Pseudomonas sp. + Bradyrhizobium sp. reduced the relative density of fibrous roots (nutrient absorption). Our work suggests that the role of bacteria in the early symbiotic stages of ECM colonization involves both the mycorrhizal symbiosis rate and plant root development processes, both essential for improve the quality of truffle-inoculated seedlings produced in commercial nurseries.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Bacteria Inoculation on Colonization of Roots by Tuber melanosporum and Growth of Quercus ilex Seedlings

Giorgi,

Amicucci,

Landi

et al. 2024

Plants

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“…Unravelling the links is challenged by the fact that bacterial composition is independent of the maturation stage and varies between regions (Niimi et al 2021 ). Therefore, the implementation of different soil conditions and different bacteria (and fungi) to support the growth of other high-prized truffle species (Monaco et al 2020 ; Piñuela et al 2020 ) in WT plantations would complement our current, albeit still descriptive, knowledge. So far, the dominant bacterial genera include (but are not limited to) Sinorhizobium , Rhizobium , Bradyrhizobium , and Pseudomonas (Barbieri et al 2007 ), which mainly fix nitrogen from the atmosphere into the soil (TN 0.24%; Fig.…”

Section: Edaphic Variationmentioning

confidence: 99%

Sustainable cultivation of the white truffle (Tuber magnatum) requires ecological understanding

2023

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The white truffle (Tuber magnatum Picco.; WT) is the most expensive and arguably also the most delicious species within the genus Tuber. Due to its hidden belowground life cycle, complex host symbiosis, and yet unknown distribution, cultivation of the enigmatic species has only recently been achieved at some plantations in France. A sustainable production of WTs under future climate change, however, requires a better ecological understanding of the species’ natural occurrence. Here, we combine information from truffle hunters with a literature review to assess the climatic, edaphic, geographic, and symbiotic characteristics of 231 reported WT sites in southeast Europe. Our meta-study shows that 75% of the WT sites are located outside the species’ most famous harvest region, the Piedmont in northern Italy. Spanning a wide geographic range from ~ 37° N in Sicily to ~ 47° N in Hungary, and elevations between sea level in the north and 1000 m asl in the south, all WT sites are characterised by mean winter temperatures > 0.4 °C and summer precipitation totals of ~ 50 mm. Often formed during past flood or landslide events, current soil conditions of the WT sites exhibit pH levels between 6.4 and 8.7, high macroporosity, and a cation exchange capacity of ~ 17 meq/100 g. At least 26 potential host species from 12 genera were reported at the WT sites, with Populus alba and Quercus cerris accounting for 23.5% of all plant species. We expect our findings to contribute to a sustainable WT industry under changing environmental and economic conditions.

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“…The physiology and development of the host plant and its accompanying fungus strongly depend on the associated microbiome under both natural and nursery conditions [ 24 , 25 ]. Plants host a fascinating diversity of microbes inside and around their roots, aka the microbiome [ 26 ], which aid in absorbing nutrients and procuring the necessary elements or regulating hormonal imbalance [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

The Microbiome Structure of the Symbiosis between the Desert Truffle Terfezia boudieri and Its Host Plant Helianthemum sessiliflorum

Satish

Barak

Keren

et al. 2022

JoF

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The desert truffle Terfezia boudieri is an ascomycete fungus that forms ect-endomycorrhiza in the roots of plants belonging to Cistaceae. The fungus forms hypogeous edible fruit bodies, appreciated as gourmet food. Truffles and host plants are colonized by various microbes, which may contribute to their development. However, the diversity and composition of the bacterial community under field conditions in the Negev desert are still unknown. The overall goal of this research was to identify the rhizosphere microbial community supporting the establishment of a symbiotic association between T. boudieri and Helianthemum sessiliflorum. The bacterial community was characterized by fruiting bodies, mycorrhized roots, and rhizosphere soil. Based on next-generation sequencing meta-analyses of the 16S rRNA gene, we discovered diverse bacterial communities of fruit bodies that differed from those found in the roots and rhizosphere. Families of Proteobacteria, Planctomycetes, and Actinobacteria were present in all four samples. Alpha diversity analysis revealed that the rhizosphere and roots contain significantly higher bacterial species numbers compared to the fruit. Additionally, ANOSIM and PCoA provided a comparative analysis of the bacterial taxa associated with fruiting bodies, roots, and rhizosphere. The core microbiome described consists of groups whose biological role triggers important traits supporting plant growth and fruit body development.

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Use of Inoculator Bacteria to Promote Tuber melanosporum Root Colonization and Growth on Quercus faginea Saplings

Cited by 9 publications

References 38 publications

Effect of Bacteria Inoculation on Colonization of Roots by Tuber melanosporum and Growth of Quercus ilex Seedlings

Effect of Bacteria Inoculation on Colonization of Roots by Tuber melanosporum and Growth of Quercus ilex Seedlings

Sustainable cultivation of the white truffle (Tuber magnatum) requires ecological understanding

The Microbiome Structure of the Symbiosis between the Desert Truffle Terfezia boudieri and Its Host Plant Helianthemum sessiliflorum

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