Use of ISSR profiles and ITS-sequences to study the biogeography of alpine cushion plants in the genus Raoulia (Asteraceae)

Smissen, Rob D.; Breitwieser, Ilse; Ward, Josephine M.; McLenachan, Patricia A.; Lockhart, P. J.

doi:10.1007/s00606-002-0249-2

Cited by 27 publications

(36 citation statements)

References 12 publications

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“…Because of these reasons, ISSR are being used for population authentication and population molecular ecology studies. [8][9][10][11][12][13][14][15][16] The present study was undertaken with the objective of establishing specific molecular markers using ISSR for authenticating eight wild D. officinale populations. …”

mentioning

confidence: 99%

Intersimple Sequence Repeats (ISSR) Molecular Fingerprinting Markers for Authenticating Populations of Dendrobium officinale KIMURA et MIGO

Shen

Ding

Liu

et al. 2006

Biological & Pharmaceutical Bulletin

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(previous name: Dendrobium candidum WALL. ex LINDL. 2)) is an endangered species in China and ranked "the first of the nine Chinese fairy herbs." The stems of D. officinale have been used as a traditional Chinese tonic medicine called Tiepi Fengdou 3) for hundreds of years. It can benefit human health in many aspects, such as nourishing yin and clearing away unhealthy heat, benefiting the stomach, promoting the production of body fluid, resisting cancer and prolonging life 1,3) This treasured herb is in severe shortage, making it expensive; the highest grade costs ¥12000-30000 per kg. 4)In our previous studies, rDNA ITS regions of D. officinale and other four adulterant species were sequenced. A pair of allele-specific diagnostic primers was designed to authenticate D. officinale from the other species based on rDNA ITS sequences of D. officinale and the other 37 species of Dendrobium.5,6) Recently, we have focused on the authentication of D. officinale populations using DNA molecular markers, which will lay the foundation for the selection of high-quality population resources.Intersimple sequence repeats (ISSR) have become a good DNA molecular marker for research on populations of the same species, a technique established based on PCR by Zietkiewicz et al. in 1994. 7) The main advantages of ISSR are: no need for DNA sequence information prior to amplification, low cost, simple operation, high stability, and abundance of genomic information. Because of these reasons, ISSR are being used for population authentication and population molecular ecology studies. [8][9][10][11][12][13][14][15][16] The present study was undertaken with the objective of establishing specific molecular markers using ISSR for authenticating eight wild D. officinale populations. MATERIALS AND METHODS Plant MaterialsAll materials used in this study were collected in China. Voucher samples were identified by Dr. Xiaoyu Ding and preserved by means of tissue culture in the College of Life Sciences, Nanjing Normal University. Wherever possible, three to five individuals were sampled from each population. The details are shown in Table 1.DNA Extraction Complete genomic DNA samples were extracted from fresh leaves using Dneasy Plant Mini Kits (QIAGEN), the concentrations of the DNA samples were measured spectrophotometrically, and each sample was diluted to 20 ng ml Ϫ1 with TE buffer for PCR amplification. Selection of Primers To select suitable primers for the study of populations of D. officinale, 76 ISSR primers, which were all purchased from Sangon Co., Ltd., China, were screened using two DNA samples. From the preliminary screening, 32 primers that could amplify visible bands were selected for further examination. Eventually, 10 ISSR primers that produced clear and reproducible bands were selected for the amplification of all DNA samples (Table 2).ISSR Profiling ISSR amplifications were carried out in a 25 ml volume containing Tris-HCl 10 mM, KCl 10 mM, (NH 4 ) 2 SO 4 8 mM (pH 9.0), MgCl 2 1.2-1.8 mM, Taq DNA polymerase1.5 U, dNTP 80 mM, pri...

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confidence: 99%

Intersimple Sequence Repeats (ISSR) Molecular Fingerprinting Markers for Authenticating Populations of Dendrobium officinale KIMURA et MIGO

Shen

Ding

Liu

et al. 2006

Biological & Pharmaceutical Bulletin

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“…(Gardner et al 2004); l6 Raoulia spp. (Smissen et al 2003; Ford unpubl. data); ll Hoheria glabrata and H. lyallii (Heenan et al 2005); V6 Coprosma spp.…”

Section: Species Conceptsmentioning

confidence: 99%

“…2A Pratia angulata and P. perpusilla (Murray et al 2004); 2 'Anaphalioides hookeri (inferred parentage A bellidioides andA trinervis; Smissen et al 2003;Smissen unpubl. ;Breitwieser et al 1999); 2& Ranunculus nivicola (Carter 2006);27 Six species oîAsplenium are of hybrid origin (e.g.,A.…”

Section: Species Conceptsmentioning

confidence: 99%

“…Some putative hybrids have been tested by rigorous morphological analysis (McKenzie 2001 ;McKenzie et al 2003McKenzie et al , 2004 and others are supported by the display of additive combinations of nrDNAITS sequences (Smissen et al 2003;Smissenunpubl.). The intergeneric status of many of the hybrids in the Raouîia alliance reflects marked morphological divergence between many of the species involved rather than phylogenetic relationships, as generic boundaries in the Gnaphalieae have not been aligned with robust phylogenetic analyses (the papers of Bayer et al 2000Bayer et al , 2002Breitwieser et al 1999 not withstanding).…”

Section: Historical Gene Flow (Introgression)mentioning

confidence: 99%

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A review of genetic analyses of hybridisation in New Zealand

Morgan‐Richards

Smissen

Shepherd

et al. 2009

Journal of the Royal Society of New Zealand

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Hybridisation between related taxa has a range of possible biological consequences, ranging from the production of sterile offspring, through introgression of alleles into populations, to the formation of new species. Examples of plant and animal species hybridising with related taxa abound in the New Zealand region. We review New Zealand examples of hybridisation that have been verified with chromosomal, protein or DNA data. Contemporary hybridisation has been studied at hybrid zones where distinct populations meet and mate in a defined and stable zone of contact. The role of human habitat modification is highlighted with examples of recent range changes that have led to hybridisation and subsequent conservation problems. Hybridisation can result in the swamping of endangered species, although it can also act as a bridge for the transfer of adaptations among lineages. Historical hybridisation in New Zealand has been examined with phylogenetics and there are many examples of organelle introgression or capture. The origin of new species of New Zealand stick insects, ferns and daisies via hybridisation has been demonstrated with cytogenetic and DNA sequence evidence. Thus the importance of hybridisation in the evolution of New Zealand's flora and fauna is highlighted.

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“…and the second subsequently to octoploid L. tarahaoa. Leucogenes emerges as a strongly supported monophyletic group in analyses of morphological characters , but not in nrDNA iTS or chloroplast psbA trnH DNA sequence studies (Breitwieser et al 1999;Smissen et al 2003Smissen et al ,2004.…”

Section: Introductionmentioning

confidence: 97%

Species relationships and genetic variation in the New Zealand endemicLeucogenes(Asteraceae: Gnaphalieae)

Smissen

Breitwieser

2008

New Zealand Journal of Botany

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AFLP profiles, nuclear ITS sequences, and chloroplast psbA trnH intergenic spacer sequences for representative samples of the New Zealand edelweiss species Leucogenes grandiceps, L. leontopodium, L. neglecta, and L. tarahaoa were examined. Analysis of AFLP profiles from a small number of populations strongly groups samples of each species, and in turn samples of diploid (2n = 2x = 28) L. leontopodium and tetraploid (2n = 4x = 56) L. neglecta cluster together, consistent with a derivation of the latter from the former by autopolyploidy. However, octoploid (2n = 8x = 112) L. tarahaoa does not cluster closely with either L. neglecta or L. leontopodium, indicating this species has an independent origin. Analyses of chloroplast DNA sequences show that although the majority of Leucogenes samples share similar chloroplast sequences not sampled from other species of New Zealand Gnaphalieae, a single accession of L. grandiceps and North island populations of L. leontopodium share chloroplast sequences more similar to other species of New Zealand Gnaphalieae. ITS sequence variation is more complex, with sequences sampled from Leucogenes not constituting a monophyletic group, and striking intraspecific variation detected within L. grandiceps.

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Use of ISSR profiles and ITS-sequences to study the biogeography of alpine cushion plants in the genus Raoulia (Asteraceae)

Cited by 27 publications

References 12 publications

Intersimple Sequence Repeats (ISSR) Molecular Fingerprinting Markers for Authenticating Populations of Dendrobium officinale KIMURA et MIGO

Intersimple Sequence Repeats (ISSR) Molecular Fingerprinting Markers for Authenticating Populations of Dendrobium officinale KIMURA et MIGO

A review of genetic analyses of hybridisation in New Zealand

Species relationships and genetic variation in the New Zealand endemicLeucogenes(Asteraceae: Gnaphalieae)

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