Use of lambda DNA as a marker to assess DNA stability in olive oil during storage

Spaniolas, Stelios; Bazakos, Christos; Ntourou, Thessaloniki; Bihmidine, Saadia; Georgousakis, Andreas; Kalaitzis, Panagiotis

doi:10.1007/s00217-007-0707-8

Cited by 30 publications

(20 citation statements)

References 19 publications

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“…The differential length of DNA amplicons of the trnL gene intron enabled the specific detection of 11 plant species, except for olive and avocado, whose discrimination was only possible on the basis of SNP detection. Overall results suggested that DNA barcoding can be successfully applied to the main plant oils, including all common olive oil adulterants and oils from allergenic species, as well as mixtures of them [48].…”

Section: Use Of Dna Barcoding and Genetic Traceability Of Plant-derivmentioning

confidence: 97%

“…The DNA barcoding methodology was tested for the identification of the botanical origin of plant oils (i.e., sunflower, corn, avocado, olive, sesame, soya, almond, hazelnut, walnut, cotton and wheat), implementing an analytical method for the detection of the common adulteration of olive oil with sesame oil [48]. The differential length of DNA amplicons of the trnL gene intron enabled the specific detection of 11 plant species, except for olive and avocado, whose discrimination was only possible on the basis of SNP detection.…”

Section: Use Of Dna Barcoding and Genetic Traceability Of Plant-derivmentioning

confidence: 99%

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DNA Barcoding as a Molecular Tool to Track Down Mislabeling and Food Piracy

2015

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DNA barcoding is a molecular technology that allows the identification of any biological species by amplifying, sequencing and querying the information from genic and/or intergenic standardized target regions belonging to the extranuclear genomes. Although these sequences represent a small fraction of the total DNA of a cell, both chloroplast and mitochondrial barcodes chosen for identifying plant and animal species, respectively, have shown sufficient nucleotide diversity to assess the taxonomic identity of the vast majority of organisms used in agriculture. Consequently, cpDNA and mtDNA barcoding protocols are being used more and more in the food industry and food supply chains for food labeling, not only to support food safety but also to uncover food piracy in freshly commercialized and technologically processed products. Since the extranuclear genomes are present in many copies within each cell, this technology is being more easily exploited to recover information even in degraded samples or transformed materials deriving from crop varieties and livestock species. The strong standardization that characterizes protocols used worldwide for DNA barcoding makes this technology particularly suitable for routine analyses required by agencies to safeguard food safety and quality. Here we conduct a critical review of the potentials of DNA barcoding for food labeling along with the main findings in the area of food piracy, with particular reference to agrifood and livestock foodstuffs.

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Section: Use Of Dna Barcoding and Genetic Traceability Of Plant-derivmentioning

confidence: 97%

Section: Use Of Dna Barcoding and Genetic Traceability Of Plant-derivmentioning

confidence: 99%

DNA Barcoding as a Molecular Tool to Track Down Mislabeling and Food Piracy

2015

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“…163 Similarly, DNA was extracted and effectively analysed starting from oils stored for up to 1 year at 4 ∘ C. 164 One year of storage at 25 ∘ C under natural lighting led to good amplifications when 107 bp target sequences were searched, whereas 691 and 411 bp fragments failed to amplify after 10 and 20 days respectively. 165 Other researchers, not specifying the storage conditions, observed a negative correlation between storage time and both the quality and quantity of DNA recovered from oil. The authors showed that DNA degradation increased 1 month after oil production and suggested that this might be imputable to DNA oxidative damage potentially altering the functionality of polymerase.…”

Section: Application Of Dna Markers To Extra Virgin Olive Oil and Tabmentioning

confidence: 99%

Evolution and perspectives of cultivar identification and traceability from tree to oil and table olives by means of DNA markers

et al. 2016

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In recent years, an increasing number of typicality marks has been awarded to high-quality olive oils produced from local cultivars. In this case, quality control requires effective varietal checks of the starting materials. Moreover, accurate cultivar identification is essential in vegetative-propagated plants distributed by nurseries and is a pre-requisite to register new cultivars. Food genomics provides many tools for cultivar identification and traceability from tree to oil and table olives. The results of the application of different classes of DNA markers to olive with the purpose of checking cultivar identity and variability of plant material are extensively discussed in this review, with special regard to repeatability issues and polymorphism degree. The characterization of olive germplasm from all countries of the Mediterranean basin and from less studied geographical areas is described and innovative high-throughput molecular tools to manage reference collections are reviewed. Then the transferability of DNA markers to processed products - virgin olive oils and table olives - is overviewed to point out strengths and weaknesses, with special regard to (i) the influence of processing steps and storage time on the quantity and quality of residual DNA, (ii) recent advances to overcome the bottleneck of DNA extraction from processed products, (iii) factors affecting whole comparability of DNA profiles between fresh plant materials and end-products, (iv) drawbacks in the analysis of multi-cultivar versus single-cultivar end-products and (v) the potential of quantitative polymerase chain reaction (PCR)-based techniques. © 2016 Society of Chemical Industry.

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“…Pafundo et al, 2010 observed a significant decrease of quality of DNA extracted from olive oil, with a consequent loss of information a month later from olive oil production. Spaniolas et al (2008b) has used lambda DNA as a marker to monitor the length of DNA fragments in olive oil during storage time when determined the varietal origin of olive oil. Lambda DNA is a linear molecule of approximately 50 kb, a length that probably resembles that of olive DNA present in olive oil.…”

Section: Dna-based Markersmentioning

confidence: 99%