Use of lectins in immunohematology

Gorakshakar, Ajit; Ghosh, Kanjaksha

doi:10.4103/0973-6247.172180

Cited by 32 publications

(27 citation statements)

References 92 publications

(71 reference statements)

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“…Its interaction with the glycan is similar to that between antigen and antibody or enzyme and substrate [14]. The lectin's selectivity and specificity is the basis for its use in immunohematology, for example, as the marker for human blood typing [15]. The lectin family can be classified based on the structure of its carbohydrate recognition domain (CRD) or by the structural fold/feature.…”

Section: Introductionmentioning

confidence: 99%

Lectins from the Edible Mushroom Agaricus bisporus and Their Therapeutic Potentials

Ismaya¹,

Tjandrawinata²,

Rachmawati

2020

Molecules

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The mushroom Agaricus bisporus secretes biologically active compounds and proteins with benefits for human health. Most reported proteins from A. bisporus are tyrosinases and lectins. Lectins are of therapeutic or pharmaceutical interest. To date, only limited information is available on A. bisporus lectins and lectin-like proteins. No therapeutic products derived from A. bisporus lectin (ABL) are available on the market despite its extensive exploration. Recently, A. bisporus mannose-binding protein (Abmb) was discovered. Its discovery enriches the information and increases the interest in proteins with therapeutic potential from this mushroom. Furthermore, the A. bisporus genome reveals the possible occurrence of other lectins in this mushroom that may also have therapeutic potential. Most of these putative lectins belong to the same lectin groups as ABL and Abmb. Their relationship is discussed. Particular attention is addressed to ABL and Abmb, which have been explored for their potential in medicinal or pharmaceutical applications. ABL and Abmb have anti-proliferative activities toward cancer cells and a stimulatory effect on the immune system. Possible scenarios for their use in therapy and modification are also presented.

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Section: Introductionmentioning

confidence: 99%

Lectins from the Edible Mushroom Agaricus bisporus and Their Therapeutic Potentials

Ismaya¹,

Tjandrawinata²,

Rachmawati

2020

Molecules

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“…Neurovascular staining of whole depot scWAT was performed by co-staining with a pan-neuronal markers and IB4, a marker for vasculature, as it binds to erythrocytes and endothelial cells [48-52] and effectively marks vessels smaller than 200um in diameter. Wholemount axillary scWAT (a-scWAT) tiled z-max projections exposed a dense vascular network residing in the a-scWAT depot (Fig.…”

Section: Resultsmentioning

confidence: 99%

Visualization and Analysis of Whole Depot Adipose Tissue Neural Innervation

Willows

Blaszkiewicz

Lamore

et al. 2019

Preprint

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53Adipose tissue requires neural innervation in order to regulate important metabolic functions. 54Though seminal work on adipose denervation has underscored the importance of adipose-nerve 55 interactions in both white (energy storing) and brown (energy expending) adipose tissues, much 56 remains a mystery. This is due, in part, to the inability to effectively visualize the various nerve 57 subtypes residing within these tissues and to gain a comprehensive quantitation of neurite 58 density in an entire depot. With the recent surge of advanced imaging techniques such as light 59 sheet microscopy and optical clearing procedures, adipose tissue imaging has been 60 reinvigorated with a focus on three-dimensional analysis of tissue innervation. However, 61 clearing techniques are time consuming, often require solvents caustic to objective lenses, alter 62 tissue morphology, and greatly reduce fluorophore lifespan. Not only are current methods of 63 imaging wholemount adipose tissues inconvenient, but often attempts to quantify neurite density 64 across physiological or pathophysiological conditions have been limited to representative 65 section sampling. We have developed a new method of adipose tissue neurite imaging and 66 quantitation that is faster than current clearing-based methods, does not require caustic 67 chemicals, and leaves the tissue fully intact. Maintenance of a fully intact depot allowed for tiling 68 z-stacks and producing maximum intensity projections of the entire adipose depot, which were 69 then used to quantify neurite density across the tissue. With this processing method we were 70 able to characterize the nerves, nerve-subtypes, and neurovascular interactions within the 71 inguinal subcutaneous white adipose tissue in mice using up to five fluorescent channels at high 72 resolution. We also utilized second harmonic generation, which provides label-free imaging, to 73 investigate collagen fiber abundance in adipose of obese mice. 74 75 76 77 78 79 80 81 82 83 84 85 86 87 Introduction: 88Historically overlooked as a location of diverse peripheral innervation [1], the adipose organ 89 was most prominently inspected for innervation in the mid 1960's when sympathetic nerve fibers 90 were visualized within brown adipose tissue (BAT) [2]. BAT nerves were later comprehensively 91 investigated by T.J. Bartness [3]. Energy expending BAT was the first adipose tissue to be 92 identified as being highly innervated due to its important role in thermogenesis [4], which 93 requires significant sympathetic input [2, 5, 6]. Sensory innervation has also been documented 94 in BAT, particularly around vasculature, and has been proposed to play a role in lipolysis [7]. 95More recently white adipose tissue (WAT), associated more with energy storage, was 96 demonstrated to be highly innervated by sympathetic [8, 9] and sensory nerves [5, 10], but not 97 parasympathetic [11] nerves. 98In order to visualize adipose innervation, it had been common practice to slice adipose 99 tissue into 7um-10um thick sections and...

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“…We developed integrated protocols for common sample preparation requirements including plasma separation from whole blood, RBC lysis, and DNA isolation on the same cartridge. For plasma separation, we utilized a biochemical method which is based on lectin-induced agglutination to facilitate clumping of the RBCs directly on the cartridge [23]. As illustrated in Figure 3A, agglutinated red cells are no longer responsive to electrowetting, while the supernatant plasma droplets from the agglutinated sample can be transported away from the agglutinated red blood cells.…”

Section: Plasma Preparationmentioning

confidence: 99%

Digital Microfluidic Platform to Maximize Diagnostic Tests with Low Sample Volumes from Newborns and Pediatric Patients

Sista

Nuffer

et al. 2020

Diagnostics

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“Children are not tiny adults” is an adage commonly used in pediatrics to emphasize the fact that children often have different physiological responses to sickness and trauma compared to adults. However, despite widespread acceptance of this concept, diagnostic blood testing is an excellent example of clinical care that is not yet customized to the needs of children, especially newborns. Cumulative blood loss resulting from clinical testing does not typically impact critically ill adult patients, but can quickly escalate in children, leading to iatrogenic anemia and related comorbidities. Moreover, the tests prioritized for rapid, near-patient testing in adults are not always the most clinically relevant tests for children or newborns. This report describes the development of a digital microfluidic testing platform and associated clinical assays purposely curated to address current shortcomings in pediatric laboratory testing by using microliter volumes (<50 µL) of samples. The automated platform consists of a small instrument and single-use cartridges, which contain all reagents necessary to prepare the sample and perform the assay. Electrowetting technology is used to precisely manipulate nanoliter-sized droplets of samples and reagents inside the cartridge. To date, we have automated three disparate types of assays (biochemical assays, immunoassays, and molecular assays) on the platform and have developed over two dozen unique tests, each with important clinical application to newborns and pediatric patients. Cell lysis, plasma preparation, magnetic bead washing, thermocycling, incubation, and many other essential functions were all performed on the cartridge without any user intervention. The resulting assays demonstrate performance comparable to standard clinical laboratory assays and are economical due to the reduced hands-on effort required for each assay and lower overall reagent consumption. These capabilities allow a wide range of assays to be run simultaneously on the same cartridge using significantly reduced sample volumes with results in minutes.

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Use of lectins in immunohematology

Cited by 32 publications

References 92 publications

Lectins from the Edible Mushroom Agaricus bisporus and Their Therapeutic Potentials

Lectins from the Edible Mushroom Agaricus bisporus and Their Therapeutic Potentials

Visualization and Analysis of Whole Depot Adipose Tissue Neural Innervation

Digital Microfluidic Platform to Maximize Diagnostic Tests with Low Sample Volumes from Newborns and Pediatric Patients

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