Use of liquid culture medium and ex vitro rooting for micropropagation of Acacia nilotica (L.) Del. ssp. cupressiformis

Abstract

A micro-propagation method using liquid culture medium has been developed for Acacia nilotica ssp. cupressiformis. Nodal segments obtained from mature tree (15-20 years old) was used as explants for bud breaking and multiple shoot induction. MS liquid medium with 2.0 mg l -1 of 6-benzylaminopurine (BAP) was the best for shoot bud induction. Further shoots multiplication was achieved by subculturing of in vitro produced shoot clumps (2-3 shoots) in liquid medium supplemented with various concentrations of BAP. Maximum number of shoots (12.57 ± 0.37) and shoot length (10.11 ± 0.42) were recorded in MS liquid medium supplemented with 1.0 mg l -1 of BAP. About 90 % shoots rooted ex-vitro on soilrite within 3-4 weeks when base (3-5 mm) of shoots was treated with 300 mg l -1 of indole-3-butyric acid for 5 min. The plantlets were hardened successfully in the green house and transferred to the polybags. To the best of our knowledge, this is the first report of a micro-propagation protocol for A. nilotica ssp. cupressiformis.

Enhanced micropropagation protocol of Morinda citrifolia L. through nodal explants

“…The multiplication of shoots in liquid media has several advantages, such as fast rate of multiplication, lack of impurities from agar, dilution of exudates from the explants, and uniform dispersal and better availability of nutrients and growth regulators (Lilia et al, 2002;Ascough and Fennell, 2004;Kalpana et al, 2009;Shekhawat et al, 2014). The liquid medium has been used successfully in various plants systems, especially for micropropagation of tree species (Dandin and Murthy, 2012;Rathore et al, 2014Rathore et al, , 2015. Morphological deformity and deterioration of cultures were not observed in this study; therefore liquid media system could be used in continuous multiplication of shoots of M. citrifolia.…”

“…Subramani et al (2007) observed rhizogenic calli on MS media with the combination of cytokinin and auxin. Use of IBA as better growth regulator for in vitro root induction has been reported by various researchers in many plant species (Ganesh and Sreenath, 2000;Kavitha et al, 2009;Saha et al, 2010;Nair et al, 2012;Rathore et al, 2014Rathore et al, , 2015Ozel et al, 2015). The rooting Note: Mean separation was analyzed by ANOVA using SPSS software (var.…”

“…Ex vitro root induction was successfully proved by many researchers in Ceropegia bulbosa (Phulwaria et al, 2013), Momordica dioica , Caralluma edulis (Patel et al, 2014), Vitex trifolia (Ahmed and Anis, 2014), Acacia nilotica (Rathore et al, 2014(Rathore et al, , 2015 etc. It is a cost effective technique and could save time and energy in plant propagation system (Yan et al, 2010;Ranaweeraa et al, 2013).…”