Use of liquid scintillation quenching to quantitate lipid mass

Snyder, Fred; Moehl, Anita

doi:10.1016/0003-2697(69)90205-x

Cited by 10 publications

(5 citation statements)

References 6 publications

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“…Completeness of the lipid extraction procedure was checked by measuring the quantity of fatty acids released after the extracted residue from one whole brain and from one batch of membranes saponified with 10% K O H in 75% aqueous ethanol. After acidification, the fatty acids were extracted and then quantitated by two independent procedures for lipid mass after resolution by thin-layer chromatography: photodensitometry (Privett et a/., 1965) and scintillation quenching (Snyder and Moehl, 1969). Both methods showed less than 0.5% fatty acids present in both tissue residues and indicated that the Bligh and Dyer extraction procedure was quantitative for removing lipids from rat brain.…”

Section: Methodsmentioning

confidence: 99%

“…The lipid extracts were resolved into neutral lipid classes by thin-layer chromatography on silica gel G in hexane-diethyl ether-acetic acid (80:20:1, v/v). Only free sterols were found in significant amounts in the neutral lipid fraction; they were resolved by thin-layer chromatography and quantitated by photodensi-tometry (Privett et nl., 1965) and liquid scintillation quenching (Snyder and Moehl, 1969 Rouser et al (1966). The identification of the ceramide and cerebroside was based on thin-layer chromatography in diethyl ether; cholesterol migrated at a higher RF than the ceramide.…”

Section: Methodsmentioning

confidence: 99%

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Lipid composition of synaptic plasma membranes isolated from rat brain by zonal centrifugation

Cotman¹,

Blank²,

Moehl³

et al. 1969

Biochemistry

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236

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A B s m A c T : A plasma membrane fraction derived from rat brain nerve endings or the synaptic plasma membrane fraction, isolated by zonal centrifugation, was analyzed for their lipid content and their composition of lipid classes and their aliphatic moieties. With the exception of glycolipids and sphingomyelin, the lipid classes of synaptic plasma membrane are very similar to whole brain. Phosphatidylcholine and phosphatidylethanolamine are the major lipids and make up 6 2 z of the total membrane lipid. Cholesterol and ceramide make up the major neutral lipids and 2 1 z of total brain lipid. The synaptic plasma membranes contain lower quantities of glyceryl ethers in phosphatidylethanolamine and phosphatidylcholine fractions than in whole brain. Certain distinctive features in fatty acid content were T he components of neuronal membranes need to be known because elucidation of the structure and dynamic function of this membrane is dependent upon such knowledge. Specific lipids of membranes undoubtedly subserve specific functions ; studies of model membranes and experiences with enzyme reconstitution illustrate the vital role and specificity of lipids in the functional properties of biomembranes. Tetrodotoxin, a molecule that specifically blocks changes in sodium conductance during nerve action potentials, interacts with and causes spreading of surface films made from nonpolar lipids extracted from squid axons, but polar lipids do not show such interactions (Villegas and Camejo, 1968;Camejo and Villegas, 1969). Phospholipids are essential for respiratory activity in mitochondria (Fleischer er a/.,

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Lipid composition of synaptic plasma membranes isolated from rat brain by zonal centrifugation

Cotman¹,

Blank²,

Moehl³

et al. 1969

Biochemistry

Self Cite

236

View full text Add to dashboard Cite

show abstract

“…This technique is used to determine 10 to 100 pg of lipids (192) and the concentration of fat in milk (198). Both studies employ conventional counting equipment.…”

Section: Radioisotopes In Chemical Analysismentioning

confidence: 99%

“…Radioisotopic light sources, prepared from 14C in liquid scintillators, have been used for digital photometric measurements. This technique is used to determine 10 to 100 µg of lipids (192) and the concentration of fat in milk (198). Both studies employ conventional counting equipment.…”

Section: Liquid Scintillationmentioning

confidence: 99%

Nucleonics

Lyon¹,

Ricci²,

Ross³

1970

Anal. Chem.

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“…However even recrystallization is not always proof for purity of compounds, especially if the compounds are very similar in their chemical structures (l). Methods have been published on determination of specific radioactivity using thin layer chromatograph (TLC) scraping, counting and quantitation by colorimetry (2,3). These methods are either very complicated or not in general use.…”

mentioning

confidence: 99%