Use of low-power He-Ne laser therapy to accelerate regeneration processes of injured sciatic nerve in rabbit

Al-Shammari, Ahmed Majeed; Syhood, Yahya; Al-Khafaji, Ahmed S.

doi:10.1186/s41983-018-0047-6

Cited by 18 publications

(10 citation statements)

References 28 publications

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“…The work was conducted under general anaesthesia by intramuscular injection according to the weight of each animal (ketamine 10%, 0.35 ml/kg; Kepro, Holland) and xylazine 20%, 0.5 ml/kg, Kepro, Holland) at the back of the leg. Less than 15 min was the needed time to achieve anesthesia [17]. The right limb of the rabbit was shaved, and the skin was cleaned with alcohol and iodine.…”

Section: Methodsmentioning

confidence: 99%

In vitro periodontal ligament cell expansion by co-culture method and formation of multi-layered periodontal ligament-derived cell sheets

Safi

Hussein

Al-Shammari

2019

Regenerative Therapy

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ObjectivePer-implantitis is one of the implant treatment complications. Dentists have failed to restore damaged periodontium by using conventional therapies. Tissue engineering (stem cells, scaffold and growth factors) aims to reconstruct natural tissues. The paper aimed to isolate both periodontal ligament stem cells (PDLSCs) and bone marrow mesenchymal stem cells (BMMSCs) and use them in a co-culture method to create three-layered cell sheets for reconstructing natural periodontal ligament (PDL) tissue.Materials and methodsBMMSCs were isolated from rabbit tibia and femur, and PDLSC culture was established from the lower right incisor. The cells were co-cultured to induce BMMSC differentiation into PDL cells. Cell morphology, stem cells and PDL-specific markers (CD90, CD34, and periostin) were also detected using immunofluorescent assay. Co-cultured cell monolayers were detached using temperature-responsive tissue culture dishes and collagen graft to create the three-layer construct. The 3D-engineered tissue was examined histologically and by field emission scanning electron microscopy (FESEM).ResultsBMMSCs co-cultured with PDLSCs successfully induced more PDL cells. The newly induced PDL cells exhibited periostin and CD90 expression. Fluorescence green intensity was measured for the co-cultured cells that were stained with periostin, the mean fluorescence green intensity (periostin expression) was significantly higher for the newly induced PDL cells after 1, 2, and 3 weeks when compared with control (BM-MSCs), at 21 days non-significant difference was measured when compared with control (PDLSCs).The results showed the successful formation of 3D multilayer PDL tissue. Histological cross-section showed cell sheets and the stable adhesion between them. FESEM examination was conducted for the cross-section, showing three-layered cell sheets with stable adhesion between cells.ConclusionsThe results of this paper report that the three layered-cell sheets were successfully constructed by the novel use of collagen graft as a scaffold to be used in treatment of periodontitis and to envelop the dental implants to create biohybrid implant.

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Section: Methodsmentioning

confidence: 99%

In vitro periodontal ligament cell expansion by co-culture method and formation of multi-layered periodontal ligament-derived cell sheets

Safi

Hussein

Al-Shammari

2019

Regenerative Therapy

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“…The treated and untreated cells were photographed at a magnification of 200x at four haphazardly selected cultured fields using an inverted light microscope (Leicamicrosystems, Germany) and a digital colour camera (Leica-microsystems, Germany) (20).…”

Section: Morphology Analysismentioning

confidence: 99%

Broad-Spectrum Cytotoxic Effect of Calendula officinalis L Against Breast Cancer Cells

2020

IJFMT

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Background: Calendula officinalis L used in Iraqi folklore medicine for several medical applications. This research evaluated the leaves extract as an anti-breast cancer agent in in-vitro cancer cell line systems and studies its active compounds. Crystal violet viability assay was used to determine the cytotoxicity of the leave methanolic extract of Calendula officinalis L against diverse breast cancer cell lines. Human breast cancer MCF7, AMJ13, MDAMB, and CAL51 cells were treated with different concentrations of extract for 72 hours. Morphological study for the exposed cell was done by examination under a phase-contrast inverted microscope. High-performance liquid chromatography (HPLC) analysis was performed to measure the concentrations of each component of phenols and flavonoids in the Calendula officinalis L extract. Results: It was found that methanolic extract of Calendula officinalis L inhibits the proliferation of all breast cancer cells significantly at the meantime; it does not affect normal embryonic cells. Additionally, it induced the cytopathic morphological changes in cancer cells. Furthermore, HPLC study revealed that Calendula officinalis L extract contained an important component of flavonoids. Conclusions: Calendula officinalis L leaves extract inhibited the proliferation of breast cancer cells especially MDAMB cells with no effect on normal cells. This work showed that Calendula officinalis L is a possible natural source as broad-spectrum anti-breast cancer drug.

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“…For statistical analysis, quantitative measurement of each picture was performed in triplicate. Percentage (%) of cells stained by crystal violet were calculated [22].…”

Section: Morphology and Quantitative Image Analysesmentioning

confidence: 99%

Selective cytotoxic effect of Plantago lanceolata L. against breast cancer cells

Alsaraf

Mohammad

Al-Shammari

et al. 2019

J Egypt Natl Canc Inst

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Background: Plantago lanceolata L. is used in Iraqi folklore medicine to treat injuries, and its extract is prescribed by some herbalists for cancer patients. This research aimed to evaluate the effect of P. lanceolata leaf extract on breast cancer cell lines in vitro and to identify its active compounds. Crystal violet viability assay was used to determine the cytotoxicity of methanolic P. lanceolata leaf extract against various breast cancer cell lines. MCF7, AMJ13, MDAMB, and CAL51 human breast cancer cells were treated with different concentrations of the extract for 72 h. The morphology of the treated cells was examined under a phase-contrast inverted microscope. The clonogenic ability was assessed through a clonogenic assay. High-performance liquid chromatography (HPLC) analysis was performed to measure the concentrations of phenols and flavonoids in the extract. Results: The methanolic P. lanceolata leaf extract significantly inhibited the proliferation of triple-negative CAL51 cells but showed minor effect on the other breast cancer cells. In addition, at high doses, it induced cytopathic morphological changes. The clonogenic assay showed low colony formation in the exposed cells, especially CAL51 cells. Furthermore, HPLC study revealed that the methanolic extract contained important flavonoid glycosides, especially rutin, myricetin quercetin, and kaempferol. Conclusions: P. lanceolata leaf extract selectively inhibited the proliferation of CAL51 triple-negative breast cancer cells and showed minor effect on the other breast cancer cells types studied. Thus, this study showed P. lanceolata as a possible natural source of selective anti-triple-negative breast cancer drugs.

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Use of low-power He-Ne laser therapy to accelerate regeneration processes of injured sciatic nerve in rabbit

Cited by 18 publications

References 28 publications

In vitro periodontal ligament cell expansion by co-culture method and formation of multi-layered periodontal ligament-derived cell sheets

In vitro periodontal ligament cell expansion by co-culture method and formation of multi-layered periodontal ligament-derived cell sheets

Broad-Spectrum Cytotoxic Effect of Calendula officinalis L Against Breast Cancer Cells

Selective cytotoxic effect of Plantago lanceolata L. against breast cancer cells

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