Use of &lt;em&gt;Alu&lt;/em&gt; Element Containing Minigenes to Analyze Circular RNAs

Welden, Justin R.; Pawluchin, Anna; Doorn, Jacob van; Stamm, Stefan

doi:10.3791/59760

Cited by 3 publications

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“…According to the construction sequence, circRNA can be divided into three categories: intron circRNA, exon circRNA, and exon-intron circRNA. All kinds of circRNA are produced in different ways, with different sequences and structures, so their biological functions are different [ 14 , 15 ]. At present, its physiological function and mechanism are still unclear, and what role it plays in various diseases is rarely reported.…”

Section: Introductionmentioning

confidence: 99%

The Regulation of circRNA RNF13/miRNA-1224-5p Axis Promotes the Malignant Evolution in Acute Myeloid Leukemia

Zhang

Wang

et al. 2020

BioMed Research International

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Objective. To study the biological function of circular RNA RNF13 (circRNF13) in acute myeloid leukemia (AML) and its relationship with prognosis. Methods. We constructed stable AML cell lines with downregulated expression of circRNF13, and then, we explored the effect of downregulation of circRNF13 expression on the proliferation, migration, and invasion through qRT-PCR, MTT curve, colony formation, transwell migration and invasion experiment, cell cycle, apoptosis, Caspase 3/7 assay, and other experiments. We also studied the expression of C-myc and Tenascin-C by qRT-PCR to explore the role of circRNF13. Results. When the expression of circRNF13 was downregulated, the proliferation rate of AML cells decreased significantly, the cell cycle was blocked to G1 phase, and apoptosis rate increased significantly. C-myc related to cell proliferation decreased significantly at RNA level. Furthermore, when the expression of circRNF13 was downregulated, the migration and invasion ability of AML cells was significantly reduced, and the expression of Tenascin-C related to migration and invasion also decreased significantly. The luciferase reporter assay system confirmed that miRNA-1224-5p was the direct target of circRNF13. Conclusion. CircRNF13 inhibited the proliferation, migration, and invasion of AML cells by regulating the expression of miRNA-1224-5p. This study provides some clues for the diagnosis and treatment of AML.

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Section: Introductionmentioning

confidence: 99%

The Regulation of circRNA RNF13/miRNA-1224-5p Axis Promotes the Malignant Evolution in Acute Myeloid Leukemia

Zhang

Wang

et al. 2020

BioMed Research International

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The design and synthesis of circular RNAs

Obi

Chen

2021

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Circular versus linear RNA topology: different modes of RNA–RNA interactions in vitro and in human cells

et al. 2021

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Circular RNA is progressively reported to occur in various species including mammals where it is thought to be involved in the post-transcriptional regulation of gene expression, partly via interactions with microRNA. Here, we asked whether the circular topology causes functional differences to linear forms when interacting with short RNA strands in vitro and in human cells. Kinetic studies with human bladder cancer-derived synthetic circular RNA versus linear transcripts, respectively, with short oligoribonucleotides showed similar association rates for both topologies. Conversely, a substantial topology-related difference was measured for the activation entropy and the activation enthalpy of RNA–RNA annealing. This finding strongly indicates a significant difference of the mechanism of RNA–RNA interactions. To investigate whether these characteristics of circular RNA are biologically meaningful we performed transient transfection experiments with a microRNA-regulated expression system for luciferase in bladder cancer-derived cells. We co-transfected linear or circular RNA containing one microRNA binding site for the target-suppressing microRNA mlet7a. Here, the circular isoform showed a strongly increased competition with microRNA function versus linear versions. In summary, this study suggests novel topology-related characteristics of RNA–RNA interactions involving circRNA in vitro and in living cells.

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Use of <em>Alu</em> Element Containing Minigenes to Analyze Circular RNAs

Cited by 3 publications

References 0 publications

The Regulation of circRNA RNF13/miRNA-1224-5p Axis Promotes the Malignant Evolution in Acute Myeloid Leukemia

The Regulation of circRNA RNF13/miRNA-1224-5p Axis Promotes the Malignant Evolution in Acute Myeloid Leukemia

The design and synthesis of circular RNAs

Circular versus linear RNA topology: different modes of RNA–RNA interactions in vitro and in human cells

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