Use of Lysostaphin in the Isolation of Highly Polymerized Deoxyribonucleic Acid and in the Taxonomy of Aerobic
            <i>Micrococcaceae</i>

Klesius, Phillip H.; Schuhardt, V. T.

doi:10.1128/jb.95.3.739-743.1968

Cited by 44 publications

(23 citation statements)

References 18 publications

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“…Determination of guanosine-cytidine percentages. Strains identified as Staphylococcus were treated with lysostaphin (Schwartz/Mann, Orangeburg, N.Y.) according to Klesius and Schuhardt (13), and the base composition of the recovered deoxyribonucleic acid was estimated from its buoyant density in a cesium chloride gradient by using a Spinco model E analytical centrifuge.…”

Section: Methodsmentioning

confidence: 99%

Stomach Fermentation in East African Colobus Monkeys in Their Natural State

Ohwaki¹,

Hungate²,

Lotter³

et al. 1974

Applied Microbiology

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The microbial fermentation in the stomachs of two monkeys, Colobus polykomos, collected in Kenya, was studied. The gas accumulated within the stomach contained H2 but no CH,. Volatile fatty acid concentrations were high, but accumulated acid prevented determination of the fermentation rate in untreated, incubated stomach contents. Upon addition of bicarbonate, a very rapid rate could be demonstrated. Some D-and L-lactate were in the stomach contents. Starchy seeds or fruits rather than leaves appeared to have been consumed. Microscopically, the most prominent microorganisms seen were large, very refringent cocci, possibly Sarcina ventriculi, and various smaller cocci and rods. The 28 cultured strains of bacteria included 14 Staphylococcus, 2 Streptococcus, 10 Propionibacterium, and 2 Peptostreptococcus. The culture count constituted 10 to 20% of the direct count. No protozoa or cellulolytic bacteria were found.

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Section: Methodsmentioning

confidence: 99%

Stomach Fermentation in East African Colobus Monkeys in Their Natural State

Ohwaki¹,

Hungate²,

Lotter³

et al. 1974

Applied Microbiology

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“…Lysostaphin susceptibility is a property directly related to the cell wall peptidoglycan composition of staphylococci (Browder ef al. 1965;Klesius & Schuhardt 1968). This enzyme only lyses cocci with a low G + C content (Klesius & Schuhardt 1968;Lachica et ul.…”

Section: Discussionmentioning

confidence: 99%

“…1965;Klesius & Schuhardt 1968). This enzyme only lyses cocci with a low G + C content (Klesius & Schuhardt 1968;Lachica et ul. 1971).…”

Section: Discussionmentioning

confidence: 99%

“…The lysis is caused mainly by the action of a peptidase which attacks glycyl-glycine linkages in the cross-bridges of the staphylococcal cell wall peptidoglycan. All staphylococci possess this type of bridge which appears to be exclusive to these micro-organisms (Browder et al 1965;Klesius & Schuhardt 1968). There is thus a high correlation between peptidoglycan composition and susceptibility to lysostaphin and this test has been found to be useful for the separation of staphylococci and micrococci (Schuhardt 1965).…”

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confidence: 99%

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Sensitivity to Lysostaphin as a Criterion for the Identification of Staphylococci from Animal Origin

Menes

García

Moreno

1981

Journal of Applied Bacteriology

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A total of 195 Gram positive, catalase positive cocci, isolated from ovine mastitis, abscesses in slaughtered animals and parasitic pulmonary lesions in lambs were tested for glucose fermentation, anaerobic growth in thioglycollate medium, coagulase production and susceptibility to the lytic action of lysostaphin. On the basis of lysostaphin sensitivity, 192 strains were classified as staphylococci. The number of cultures able to produce acid anaerobically from glucose, or giving a positive result in the test of Evans and Kloos was lower. A good correlation among these three tests was not observed. Ninety-seven of the strains tested gave a positive coagulase reaction. Sensitivity to lysostaphin could not be used as a criterion for the differentiation of coagulase positive and coagulase negative strains. The turbidimetric method employed for the assessment of lysostaphin sensitivity is discussed.

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“…Clinical isolates of Micrococcaceae were identified as staphylococci or micrococci by Gram stain (10), catalase (10), and lysostaphin susceptibility (5). Species identification of staphylococci was determined with coagulase (10) and the API Staph-Ident System (Analytab Products, Inc., Plainview, N.J.).…”

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confidence: 99%

Abbreviated scheme for presumptive identification of Staphylococcus saprophyticus from urine cultures

Morgan¹

1983

J Clin Microbiol

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A simplified scheme for the presumptive identification of Staphylococcus saprophyticus was investigated. Clinical isolates of Micrococcaceae were tested in microtiter plates containing phenol red-novobiocin-turanose broth and phenol red-arabinose broth. Standard saline suspensions of test organisms served as inoculum. Identifying features were growth within 18 to 24 h in novobiocinturanose broth, indicating resistance to 1.6 ,ug of novobiocin per ml and fermentation of turanose. With this method, all clinical isolates of S. saprophyticus tested were correctly identified.

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Use of Lysostaphin in the Isolation of Highly Polymerized Deoxyribonucleic Acid and in the Taxonomy of Aerobic Micrococcaceae

Cited by 44 publications

References 18 publications

Stomach Fermentation in East African Colobus Monkeys in Their Natural State

Stomach Fermentation in East African Colobus Monkeys in Their Natural State

Sensitivity to Lysostaphin as a Criterion for the Identification of Staphylococci from Animal Origin

Abbreviated scheme for presumptive identification of Staphylococcus saprophyticus from urine cultures

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