Use of magnetic techniques for the isolation of cells

Šafařı́k, Ivo; Šafařı́ková, Mirka

doi:10.1016/s0378-4347(98)00338-7

Cited by 506 publications

(75 citation statements)

References 116 publications

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“…Capture time of magnetic particles in magnetic field of quadrupole, can be also decreased by increasing magnetic flux densities and field gradients of studied configuration replacing Magnetic drug delivery and targeting: principles and applications permanent magnets by pulsed electromagnets, similar to those pulsed quadrupole lenses using for focusing heavy ion beams in accelerator physics. These results equally apply also to magnetic separation of proteins, DNA, and whole cells 35,36 . The first study about the magnetic drug targeting was performed 30 years ago 37 , but the revival of the field, due to apperance of a cheap and very powerful neodymium magnets, started in the the new millenium [38][39][40][41][42][43][44] .…”

Section: Magnetic Drug Delivery and Targeting: Principles And Applicamentioning

confidence: 63%

Magnetic Drug Delivery and Targeting: Principles and Applications

Babincová¹,

Babinec²

2009

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Background: Nanomaterials are at the leading edge of the rapidly developing field of nanotechnology. Magnetic nanoparticles for cancer therapy and diagnosis have been developed on the basis of their unique physico-chemical properties not present in other materials. Their versatility is widely exploited in such diverse techniques as cell and macromolecule separation and purification, immunoassays, targeted drug delivery, controlled material release, electromagnetic hyperthermia, gene therapy, or magnetic resonance imaging. In this review we concentrate on the physical principles of magnetic drug targeting and biomedical applications of this technique. Methods and results:We examined several databases, PubMed, ISI Web of Knowledge, and Scopus, for the period 1985-2009, with specific attention to studies that used targeting of magnetic nanoparticles especially in the therapy and diagnostics of tumors. We have also presented several of our own results on theoretical simulations of magnetic particle motion in external magnetic field.Conclusions: We found growing number of published papers in this field of nanomedicine, showing the almost unlimited potential of magnetic nanoparticles in the field of experimental and clinical oncology.

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Section: Magnetic Drug Delivery and Targeting: Principles And Applicamentioning

confidence: 63%

Magnetic Drug Delivery and Targeting: Principles and Applications

Babincová¹,

Babinec²

2009

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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“…Immunomagnetic separation of cells using superparamagnetic particles or beads coated with antibodies against surface antigens has been extensively investigated [1][2][3]29]. It is a very selective and mild technology because it permits rapid and efficient isolation of target cell populations.…”

Section: Resultsmentioning

confidence: 99%

Synthesis and functionalization of superparamagnetic poly- ɛ -caprolactone microparticles for the selective isolation of subpopulations of human adipose-derived stem cells

Balmayor

Pashkuleva

Frias

et al. 2011

J. R. Soc. Interface.

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There has been a growing interest in using biofunctionalized magnetic particles for cell isolation. This paper describes the synthesis and characterization of magnetite-polymer (Fe 3 O 4 -poly-1-caprolactone, magnetite-PCL) microparticles surface functionalized with amino and epoxy groups allowing easy covalent attachment of specific antibodies and subsequent ability to bind target cells. Particles with different sizes (4 -135 mm), spherical shape and superparamagnetic behaviour (magnetite content of about 13 wt%) were obtained. The functionalized microparticles presented high protein-binding capacity (coupling efficiency of 47% for epoxy-and 71% for amino-functionalized particles) with a low level of non-specific binding. We have further investigated the influence of initial protein concentration, pH, ionic strength, temperature and incubation time on the capacity of amino-functionalized particles to bind protein molecules. The results showed that maximum protein coupling is rapidly achieved ( 5 h) at pH 5.5 and low ionic strength (0.05 M NaCl). Furthermore, when cultured in direct contact with osteoblast-like cells (Saos-2) or human-derived adipose stem cells (ASCs), the amino-functionalized particles did not affect the proliferation and morphology of the cells. As a proof of principle for the application of magnetic microparticles for cell isolation, CD105 (endoglin) antibody was coupled to the magnetic particle surface to bind subpopulations of human ASCs expressing the CD105 antigen. The isolation of CD105þ ASCs from a heterogeneous cell population was confirmed by flow cytometry analysis. Given the demonstrated potential of functionalized magnetite-PCL microparticles for selective cell isolation, we expect that these particles may be further applied in immuno-magnetic cell separation owing to their versatility and ease of surface modification.

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“…There are a few technologies namely, batch magnetic separators, flow-through magnetic separators and magnetically stabilized fluidized bed separators to isolate particles based on their differences in magnetic content. 223 However, as MTB are motile by means of flagella, 224 their motion complicates the separation process. Several groups have also described the use of microfluidic devices to deflect particles across streamlines and into different outlets based on their different magnetic contents.…”

Section: Introductionmentioning

confidence: 99%

Acute and Chronic Neural Stimulation via Mechano-Sensitive Ion Channels

Tay

2018

Springer Theses

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Neural stimulation techniques for eliciting calcium influx can elucidate the physiological roles of specific neural populations. To overcome some of the limitations of existing techniques such as poor specificity and noxious effects of heat, I developed a technology for non-invasive control of neural activities using magnetic forces and magnetic nanoparticles (MNPs) which offer deep tissue penetration and controllable dosage. Extensive investigations with different neurotoxins and experimental conditions support that the mechanism of magnetic stimulation that involves membrane-bound MNPs transducing magnetic forces into mechanical stretching of the cell membrane to enhance the opening probability of mechano-sensitive N-type calcium ion channels to induce calcium influx.Making use of the ability of neural networks to actively regulate their ratio of excitatory to inhibitory ion channel/receptor, I also performed chronic magnetic stimulation on fragile X iii syndrome (FXS) model neural networks. We found that chronic magnetic stimulation reduced the density of N-type calcium ion channels whose expression is increased in FXS. This technique demonstrates the potential of using bio-magnetic/mechanical forces to modulate expressions of mechano-sensitive ion channels where they are over-expressed in diseases such as abnormal nociception.Nonetheless, there are still a few areas where the technique can be improved. Firstly, it is the use of MNPs with more uniform properties to have greater control on magnetic stimulations.Secondly, the technique needs to be useful for in vivo studies. Therefore, I started researching on magnetotactic bacteria (MTB) which produce biological MNPs with superior properties such as uniform sizes and highly homogenous magnetic properties with the goal of harvesting MNPs from them.MTB, however, grow extremely slowly and the number of MNPs produced/bacterium is low. One way to overcome this problem is to evolve MTB over-producers of MNPs but this strategy is constrained by the absence of a selection platform that is quantitative and offer high throughput. To overcome this problem, I combined random chemical mutagenesis and selection using a magnetic ratcheting platform to generate and isolate MTB over-producers that produce twice as many MNPs/bacterium after 5 rounds of mutation/selection. I next designed a magnetic microfluidic device and demonstrate as a proof of concept, that it can be coupled to a bioreactor for high throughput microfluidic selection of MTB over-producers.iv

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Use of magnetic techniques for the isolation of cells

Cited by 506 publications

References 116 publications

Magnetic Drug Delivery and Targeting: Principles and Applications

Magnetic Drug Delivery and Targeting: Principles and Applications

Synthesis and functionalization of superparamagnetic poly- ɛ -caprolactone microparticles for the selective isolation of subpopulations of human adipose-derived stem cells

Acute and Chronic Neural Stimulation via Mechano-Sensitive Ion Channels

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