Use of MALDI-TOF for identification and surveillance of gram-negative bacteria in captive wild psittacines

Davies, Yamê Miniero; Franco, Leticia; Barbosa, Fernanda Bueno; Vanin, C. L.; Gomes, Vasco T.M.; Moreno, Luisa Zanolli; Barbosa, Mikaela Renata Funada; Sato, Maria Inês Z.; Moreno, Andréa Micke; Knöbl, Terezinha

doi:10.1590/1519-6984.233523

Cited by 3 publications

(3 citation statements)

References 38 publications

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“…However, data on its applicability reveal MALDI-TOF MS's importance in quickly typing subspecies of medical importance; the technique contributes to the proper management of antimicrobials and best hospital practices. On the other hand, its limitations highlight the need to increase reference data for reference banks (Croxatto et al, 2012;Angeletti, 2017;Davies et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Resistance determinants of emerging pathogens isolated from an intensive care unit as a parameter of population health conditions of the Legal Amazon microregion

et al. 2023

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Bacteria responsible for causing infections are common in hospital environments, water, soil, and food products. The infection risk is intensified by the absence of public sanitation, poor quality of life, and food scarcity. These external factors promote the dissemination of pathogens by direct contamination or biofilm formation. In this work, we identified bacterial isolates obtained from intensive care units in the southern region of Tocantins, Brazil. We compared matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) techniques and 16S ribosomal ribonucleic acid (rRNA) molecular analysis; we also performed phenotypic characterization. Fifty-six isolates characterized using morphotinctorial tests were classified as gram-positive (80.4%; n = 45) and gram-negative (19.6%; n = 11) and were resistant to several antibiotic classes; notably, we identified the blaOXA-23 resistance gene in the ILH10 isolate. Microbial identification using MALDI-TOF MS resulted in the identification of Sphingomonas paucimobilis and Bacillus circulans. 16S rRNA sequencing revealed four isolates belonging to the genera Bacillus and Acinetobacter. The similarity was superior to 99% for Acinetobacter schindleri in the Basic Local Alignment Search Tool (BLAST), grouped in the clade superior to 90%. Several strains isolated from intensive care units (ICU) were resistant to various antibiotic classes. These techniques allowed for the identification of several microorganisms of importance in public health, enabling improvements in human infection control and proving the quality of inputs, food, and water.

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Section: Discussionmentioning

confidence: 99%

Resistance determinants of emerging pathogens isolated from an intensive care unit as a parameter of population health conditions of the Legal Amazon microregion

et al. 2023

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“…are regarded as common commensals of the gastrointestinal tract microbiota, and in avians, the presence of Proteus sp. was assessed in the gut microbiota of clinically healthy bird species including passerines and psittacines [ 74 , 75 , 76 , 77 , 78 ].…”

Section: Discussionmentioning

confidence: 99%

Canaries’ Microbiota: The Gut Bacterial Communities along One Female Reproductive Cycle

Hattab,

Marruchella,

Sibra

et al. 2023

Microorganisms

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Investigations of bacterial communities are on the rise both in human and veterinary medicine. Their role in health maintenance and pathogenic mechanisms is in the limelight of infectious, metabolic, and cancer research. Among the most considered, gut bacterial communities take the cake. Their part in animals was assessed mainly to improve animal production, public health, and pet management. In this regard, canaries deserve attention, being a popular pet and source of economic income for bird-keepers, for whom breeding represents a pivotal point. Thus, the present work aimed to follow gut bacterial communities’ evolution along on whole reproductive cycle of 12 healthy female canaries. Feces were collected during parental care, molting, and resting phase, and submitted for 16S rRNA sequencing. Data were analyzed and a substantial presence of Lactobacillus aviarius along all the phases, and a relevant shift of microbiota during molting and rest due to an abrupt decrease of the Vermiphilaceae family were detected. Although the meaning of such change is not clear, future research may highlight unforeseen scenarios. Moreover, Lactobacillus aviarius may be deemed for normal bacteria flora restoration in debilitated birds, perhaps improving their health and productivity.

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Section: Species Prevalent Microbiota Components Referencementioning

confidence: 99%

A Sing-Along with Canaries: Gut Bacterial Microbiota along One Female Reproductive Cycle

Hattab,

Marruchella,

Sibra

et al. 2023

Preprint

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Investigation of bacterial communities is on the rise both in human and veterinary medicine. Their role in health maintenance and pathogenic mechanisms is in the limelight of infectious, metabolic, and cancer research. Among the most considered, gut bacterial communities takes the cake. Their part in animals was assessed mainly to improve animal production, public health, and pet management. In this regard, canaries deserve attention, being a popular pet and source of economic income for bird-keepers, for whom breeding represents a pivotal point. Thus, the aim of the present work was to follow gut bacterial communities’ evolution along on whole reproductive cycle of 12 healthy female canaries. Feces were collected during parental care, molting, and resting phase, and submitted for 16S rRNA sequencing. Data analysis a substantial presence of Lactobacillus aviarius along all the phases, and a relevant shift of microbiota during molting and rest due to an abrupt decrease of Vermiphilaceae family. Although the meaning of such change is not clear, future research may highlight unforeseen scenarios. Moreover, Lactobacillus aviarius may be deemed for normal bacteria flora restoration in debilitated birds, perhaps improving their health and productivity.

show abstract

Use of MALDI-TOF for identification and surveillance of gram-negative bacteria in captive wild psittacines

Cited by 3 publications

References 38 publications

Resistance determinants of emerging pathogens isolated from an intensive care unit as a parameter of population health conditions of the Legal Amazon microregion

Resistance determinants of emerging pathogens isolated from an intensive care unit as a parameter of population health conditions of the Legal Amazon microregion

Canaries’ Microbiota: The Gut Bacterial Communities along One Female Reproductive Cycle

A Sing-Along with Canaries: Gut Bacterial Microbiota along One Female Reproductive Cycle

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