Use of mass spectrometry for imaging metabolites in plants

Lee, Young Jin; Perdian, D. C.; Song, Zhihong; Yeung, Edward S.; Nikolau, Basil J.

doi:10.1111/j.1365-313x.2012.04899.x

Cited by 215 publications

(223 citation statements)

References 127 publications

(253 reference statements)

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“…In just a few years, mass spectrometry imaging (MSI) made the dream of the location of molecular compounds at the micron scale to come true [2,3]. First developed on animal slices [4,5], this emerging technique finds a growing interest in plant proteomic [6] and metabolomic [7][8][9]. Indeed, MSI is able to provide a specific spatial distribution of proteins or metabolites in tissues.…”

Section: Introductionmentioning

confidence: 99%

MALDI Mass Spectrometry Imaging for the Simultaneous Location of Resveratrol, Pterostilbene and Viniferins on Grapevine Leaves

et al. 2014

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Abstract:To investigate the in-situ response to a stress, grapevine leaves have been subjected to mass spectrometry imaging (MSI) experiments. The Matrix Assisted Laser Desorption/Ionisation (MALDI) approach using different matrices has been evaluated. Among all the tested matrices, the 2,5-dihydroxybenzoic acid (DHB) was found to be the most efficient matrix allowing a broader range of detected stilbene phytoalexins. Resveratrol, but also more toxic compounds against fungi such as pterostilbene and viniferins, were identified and mapped. Their spatial distributions on grapevine leaves irradiated by UV show their specific colocation around the veins. Moreover, MALDI MSI reveals that resveratrol (and piceids) and viniferins are not specifically located on the same area when leaves are infected by Plasmopara viticola. Results obtained by MALDI mass spectrometry imaging demonstrate that this technique would be essential to improve the level of knowledge concerning the role of the stilbene phytoalexins involved in a stress event.

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Section: Introductionmentioning

confidence: 99%

MALDI Mass Spectrometry Imaging for the Simultaneous Location of Resveratrol, Pterostilbene and Viniferins on Grapevine Leaves

et al. 2014

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“…several salient reviews of these platforms in the context of plant MSI are available [6,24,25,26]. Here we highlight the most recent applications of MALDI-MS imaging in plant tissues (Table 1) for metabolite localization in situ.…”

Section: Introductionmentioning

confidence: 99%

Matrix assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) for direct visualization of plant metabolites in situ

Sturtevant

Lee

Chapman

2016

Current Opinion in Biotechnology

135

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Direct visualization of plant tissues by matrix assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) has revealed key insights into the localization of metabolites in situ. Recent efforts have determined the spatial distribution of primary and secondary metabolites in plant tissues and cells. Strategies have been applied in many areas of metabolism including isotope flux analyses, plant interactions, and transcriptional regulation of metabolite accumulation. Technological advances have pushed achievable spatial resolution to subcellular levels and increased instrument sensitivity by several orders of magnitude. It is anticipated that MALDI-MSI and other MSI approaches will bring a new level of understanding to metabolomics as scientists will be encouraged to consider spatial heterogeneity of metabolites in descriptions of metabolic pathway regulation Keywords Food biotechnology, Plant biotechnology Disciplines Biotechnology | Chemistry | Plant BiologyComments NOTICE: This is the author's version of a work that was accepted for publication in Current Opinion in Biotechnology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Current Opinion in Biotechnology, 37, February 2016, doi: 10.1016/j.copbio.2015 Highlights:Plant chemical organization is visualized directly using MALDI-MSI.MALDI-MS imaging of plant tissues reveals unforeseen analyte compartmentalization.Analyte heterogeneity has been validated by other MS and biochemical analyses.

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“…Recently, MALDI-MSI has begun to see increased application in the study of plant biology (for review, see Kaspar et al, 2011;Lee et al, 2012). Here, we applied MALDI-MSI in combination with gas chromatographymass spectrometry (GC-MS) and HPLC-based analyses of extracts to study the distributions and profiles of metabolites in germinating maize seeds at four different time points after imbibition.…”

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confidence: 99%

Spatial Mapping and Profiling of Metabolite Distributions during Germination

et al. 2017

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Germination is a highly complex process by which seeds begin to develop and establish themselves as viable organisms. In this study, we utilize a combination of gas chromatography-mass spectrometry, liquid chromatography-fluorescence, and mass spectrometry imaging approaches to profile and visualize the metabolic distributions of germinating seeds from two different inbreds of maize (Zea mays) seeds, B73 and Mo17. Gas chromatography and liquid chromatography analyses demonstrate that the two inbreds are highly differentiated in their metabolite profiles throughout the course of germination, especially with regard to amino acids, sugar alcohols, and small organic acids. Crude dissection of the seed followed by gas chromatography-mass spectrometry analysis of polar metabolites also revealed that many compounds were highly sequestered among the various seed tissue types. To further localize compounds, matrix-assisted laser desorption/ionization mass spectrometry imaging was utilized to visualize compounds in fine detail in their native environments over the course of germination. Most notably, the fatty acyl chain-dependent differential localization of phospholipids and triacylglycerols was observed within the embryo and radicle, showing correlation with the heterogeneous distribution of fatty acids. Other interesting observations include unusual localization of ceramides on the endosperm/scutellum boundary and subcellular localization of ferulate in the aleurone.Plants use seeds as the propagule to ensure reproduction to the next generation, and over the past 10,000 years, human civilizations have established agricultural practices to ensure a seed-based food supply (Larson et al., 2014). Therefore, deciphering the processes that enable seeds to perform their biological functions is of importance in understanding how plants are propagated and also is of practical importance to improve agriculture. Seeds are designed to survive long periods of dormancy in a relatively dry state, and the process of germination is initiated by the imbibition of water. During this germination process, many metabolic changes occur, most of which are associated with the catabolism of seed storage products (proteins, polysaccharides, and lipids) into metabolically usable, simpler chemical forms that are either used as precursors to assemble the growing seedling or are oxidized via energy-producing biochemical pathways to thermodynamically support growth (Bewley, 1997(Bewley, , 2001.The specific metabolic processes that support seed germination are somewhat dependent on the taxonomic clade of the plant, which determines seed tissue/ organ organization and the nature of the seed storage compounds. Specifically, the seeds of the Poaceae family of monocots are characterized by a starch-and protein-filled endosperm, and their catabolism by digestive enzymes produced in the outermost layer of the endosperm (i.e. the aleurone) provides the carbonbased and nitrogenous precursors for the growth of the embryo, which is composed of the embryonic ax...

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Use of mass spectrometry for imaging metabolites in plants

Cited by 215 publications

References 127 publications

MALDI Mass Spectrometry Imaging for the Simultaneous Location of Resveratrol, Pterostilbene and Viniferins on Grapevine Leaves

MALDI Mass Spectrometry Imaging for the Simultaneous Location of Resveratrol, Pterostilbene and Viniferins on Grapevine Leaves

Matrix assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) for direct visualization of plant metabolites in situ

Spatial Mapping and Profiling of Metabolite Distributions during Germination

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