Use of Membrane Proteins from Salmonella gallinarum for Prevention of Fowl Typhoid Infection in Chickens

Bouzoubaa, K.; Nagaraja, K. V.; Newman, John A.

doi:10.2307/1591019

Cited by 39 publications

(23 citation statements)

References 15 publications

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“…In contrast, the protection produced by formalin-killed Salmonella harvested from either in vitro or in vivo conditions when used as vaccine candidate in chickens was found to be statistically insignificant. This is in agreement with other researchers who found that proteins (OMPs) gave better protection than formalin-killed bacteria [25]. For other organisms, such as S. Gallinarum and Pasteurella multocida, OMPs also give better protection than whole bacterins or sonicated cells [25,26].…”

Section: Salmonella Enteritidis' Vaccine Produces In Vitro and In Vivsupporting

confidence: 92%

“…This is in agreement with other researchers who found that proteins (OMPs) gave better protection than formalin-killed bacteria [25]. For other organisms, such as S. Gallinarum and Pasteurella multocida, OMPs also give better protection than whole bacterins or sonicated cells [25,26]. Immunization with Helicobacter whole-cell extracts or purified components also effectively prevented H. pylori infection in mice [27][28][29][30].…”

Section: Salmonella Enteritidis' Vaccine Produces In Vitro and In Vivsupporting

confidence: 91%

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Salmonella Enteritidis’ Vaccine Produces In Vitro and In Vivo Protection against Colonization

Elazomi¹

2017

JMEN

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Salmonella enterica can be considered as one of the most important causes of foodpoisoning with poultry thought to be the main source. Although S. Typhimurium, S. Enteritidis and the vast majority of other Salmonella serovars generally produce little systemic disease in adult chickens, they are able to colonize the alimentary tract of poultry. The two caeca are the main sites of the colonization of Salmonellae in chickens, and the bacteria can be easily harvested from the caeca for analysis. Bacterial proteins analyses utilizing SDS-PAGE showed differences between in vitro and in vivo that out of about 40 protein bands of in vitro preparation only a few (3-5) bands can be visualized from in vivo preparations. We suggested that some avian proteases might be responsible. Accordingly, and to investigate the hypothesis that an inactivated vaccine harvested from chickens is thought to be more protective than bacteria grown in broth culture, the immunogenicity of inactivated vaccines, either proteins or formalin killed bacteria (S. Enteritidis) harvested from chicken intestine and those from broth culture (in vitro), were compared using bacterial proteins or formalin killed bacteria as an orally inoculated vaccine candidate in chicken. The results demonstrated that sonicated proteins extracts in general have a stronger effect as vaccines against S. Enteritidis colonization in chickens than formalin killed bacteria. In contrast, the in vitro sonicated proteins obtained from a nutrient broth culture had a much better protective vaccine effect than the in vivo sonicated proteins preparations harvested from bacteria grown in chickens. IntroductionMany bacterial pathogens such as Clostridium, Staphylococcus, Campylobacter and many other bacterial strains are capable of causing food-poisoning, and Salmonella enterica can be considered as one of the most important causes with poultry thought to be the main source. Although S. Typhimurium, S. Enteritidis and the vast majority of other Salmonella serovars generally produce little systemic disease in adult chickens, they are able to colonize the alimentary tract of poultry, resulting in contamination of poultry carcasses and entry into the human food chain. However, there is a great demand to control food-poisoning salmonellosis at both breeder and layer levels at the national and global level in order to produce Salmonelle-free poultry products, due to the current correlation between S. Enteritidis PT4 and poultry products. Salmonellosis costs the European Union a minimum of 500-900 million Euros annually. Salmonellosis in food animals is a major target for reduction of human infection by the European Union. Legislation has been introduced to monitor the most important Salmonellae serovars. The major Salmonellae serovars of public health consequence are S. Typhimurium and S. Enteritidis (causing 15% and 60% respectively of all cases in Europe in 2002).Elazomi and Dhawi studied newly-hatched chickens infected with S. Enteritidis. They analyzed proteins of S. Enteritidis...

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Section: Salmonella Enteritidis' Vaccine Produces In Vitro and In Vivsupporting

confidence: 92%

Section: Salmonella Enteritidis' Vaccine Produces In Vitro and In Vivsupporting

confidence: 91%

Salmonella Enteritidis’ Vaccine Produces In Vitro and In Vivo Protection against Colonization

Elazomi¹

2017

JMEN

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“…Among the immunised groups, the chickens vaccinated with the ghost plus Montanide ISA TM 70 VG showed higher survival rates compared to those from chickens vaccinated with the ghost alone. Until recently, very few efforts have been directed toward the production of safe and immunogenic inactivated vaccines against fowl typhoid (Bouzoubaa et al, 1987;Bouzoubaa et al, 1989;Chaudhari et al, 2012;Jawale et al, 2014;Jawale and Lee, 2014b). The results of the current study highlight further progress in the development of inactivated S. Gallinarum vaccines.…”

Section: Discussionmentioning

confidence: 72%

Evaluation of Salmonella Gallinarum ghost formulated with Montanide™ ISA 70 VG adjuvant as a vaccine against fowl typhoid

Jawale

Somsanith

et al. 2015

Acta Veterinaria Hungarica

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Escherichia coli heat-labile enterotoxin B subunit (LTB) protein is a potent adjuvant. Salmonella Gallinarum ghosts carrying LTB (S. Gallinarum-LTB ghosts) were genetically constructed using a plasmid, pJHL187-LTB, designed for the co-expression of the LTB and E lysis proteins. This study evaluates the immunopotentiating effects of Montanide TM ISA 70 VG on S. Gallinarum-LTB ghost vaccination against fowl typhoid. Five-week-old layer chickens were injected intramuscularly with sterile PBS (non-immunised control, Group A), S. Gallinarum-LTB ghost (Group B) or S. Gallinarum-LTB ghost emulsified with Montanide TM ISA 70 VG adjuvant (Group C). Chickens from both Groups B and C showed significant induction of antigen-specific systemic IgG response compared to controls; in addition, Group C showed enhanced induction of systemic IgG response compared to Group B. We observed significant induction of antigen-specific lymphocyte proliferative response and increased mRNA levels of Th1 cytokines (IFN-γ and IL2) in both Groups B and C. Furthermore, in the challenge experiment with a virulent strain of S. Gallinarum, Group C showed higher survival rates compared with other groups. These results indicate that vaccination with the S. Gallinarum-LTB ghost in combination with Montanide TM ISA 70 VG may enhance the protective immunity against fowl typhoid.

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“…In Africa, it has been reported in many countries including Tanzania, Uganda (Okoj, 1993), Senegal (Arbelot et al, 1997), Nigeria (Sa'idu et al, 1994) and Morocco (Bouzoubaa et al, 1987). It is a septicaemic disease that affects primarily chicken and turkey, although natural infections in many other avian species have been reported (Wray et al, 1996;Shivaprasad, 1997).…”

Section: Introductionmentioning

confidence: 99%

Haematological changes in layers experimentally infected with salmonella gallinarum

Adam¹,

Adamu²,

John³

et al. 2017

Afr. J. Cell. Path

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Aim: The present study was conducted to investigate the haematological changes in layers experimentally infected with Salmonella gallinarum. Methods: A total of 20 eighteen-week-old ISA Brown layers were used for the experiment. The birds were randomly divided into two groups, infected and control, of 10 birds each. To establish the infection, each bird in the infected group was orally administered 0.5 ml of the inoculum containing 9x10 8 CFU/ml. Similarly, birds in the control group were each administered 0.5 ml normal saline only. Following the inoculation, all experimental birds were closely monitored for clinical signs of fowl typhoid. Blood samples were collected from each group at day zero (Day 0), 2, 4, 7, 14, 21, 28, 35 and 42, post-infection (pi) and used for determination of haematological parameters. By day seven post infection, all birds in the infected group showed clinical signs typical of fowl typhoid; namely weakness, ruffled feathers, huddling together, somnolence, greenish-yellow diarrhea, weight loss, drop in egg production, decrease in feed and water consumption and mortality rate (50%). There were, however, macrocytic hypochromic anaemia, leuckocytosis and heterophilia. In conclusion, the experimental Salmonella Gallinarum infection induced acute anaemia, leukocytosis, heterophilia and lymphopenia.

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Use of Membrane Proteins from Salmonella gallinarum for Prevention of Fowl Typhoid Infection in Chickens

Cited by 39 publications

References 15 publications

Salmonella Enteritidis’ Vaccine Produces In Vitro and In Vivo Protection against Colonization

Salmonella Enteritidis’ Vaccine Produces In Vitro and In Vivo Protection against Colonization

Evaluation of Salmonella Gallinarum ghost formulated with Montanide™ ISA 70 VG adjuvant as a vaccine against fowl typhoid

Haematological changes in layers experimentally infected with salmonella gallinarum

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