Use of membraneless osmosis for concentration of proteins from molecular-dispersed and colloidal-dispersed solutions (review)

Interactions and compatibility of 11 S globulin from Vicia Faba seeds and sodium salt of carboxymethylcellulose in an aqueous medium

“…Technologically, protein-polyelectrolyte interactions can be utilized for protein separation [4,5], enzyme immobilization [6,7], and drug delivery [8,9]. The complex formation of proteins and polysaccharides determine the structure and physical properties of biopolymers mixtures in quiescent state [10,11] and in a flow [12] and play an important role in protein processing in food products [11].…”

Biorefinery of functional biomolecules from algae

“…The main disadvantage of traditional aqueous two-phase systems (ATPS) is the utilization of external chemicals (organic solvents, polymers, salts, ionic liquids) which need to be back-extracted (chapter 7-8, Ventura et al, 2017). Alternatively, phase separation can be achieved without the need of external chemicals, a fractionation concept called membraneless osmosis (Antonov, 2000).…”

“…This principle has been proven for colloidal-dispersed systems such as skimmed milk, blood plasma, microbial broths, plant-based extracts and chloroplasts (Antonov, 2000).…”

“…Membraneless osmosis can be conducted at room temperature and phase formation takes place rapidly (within seconds) regardless of the system volume. Besides, the separation-concentration performance and the energy consumption are greatly improved compared to other methods, without adding chemically based polymers or salts (Antonov, 2000).…”

“…In order to achieve self-separation, the algae suspension after cell disintegration must contain the right type of molecules, above a critical concentration necessary for twophase formation. Antonov, (2000) presented an overview of phase diagrams for protein-polysaccharide pairs, among which pectin and rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase). Certain algal strains are rich in pectin-like polysaccharides (Domozych et al, 2012;Kermanshahi-pour et al, 2014), while rubisco is one of the most abundant proteins in algae.…”