Use of Methanogenic Activity Tests to Characterize Anaerobic Sludges, Screen for Anaerobic Biodegradability and Determine Toxicity Thresholds against Individual Anaerobic Trophic Groups and Species

Colleran, Emer; Concannon, Frank; Golden, T. C.; Geoghegan, F.; Crumlish, Bríd; Killilea, E.; Henry, Michael P.; Coates, John D.

doi:10.2166/wst.1992.0136

Cited by 136 publications

(91 citation statements)

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“…The bottles were closed with a septum and the headspace was flushed with N 2 for 5 min to ensure anaerobic conditions. The biogas production was determined by measuring the overpressure in the headspace with time frequency (Colleran et al, 1992). Constant agitation was provided by a shaker at 50 rpm.…”

Section: Anaerobic Digestionmentioning

confidence: 99%

Recovery of Protein Concentrates From Microalgal Biomass Grown in Manure for Fish Feed and Valorization of the By-Products Through Anaerobic Digestion

Hernández

Molinuevo-Salces

Riaño

et al. 2018

Front. Sustain. Food Syst.

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Section: Anaerobic Digestionmentioning

confidence: 99%

Recovery of Protein Concentrates From Microalgal Biomass Grown in Manure for Fish Feed and Valorization of the By-Products Through Anaerobic Digestion

Hernández

Molinuevo-Salces

Riaño

et al. 2018

Front. Sustain. Food Syst.

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“…Methanogenic activity tests were performed using the pressure transducer technique 11 (Colleran et al, 1992) to determine the chronic effect of TET on methanogenic pathway. The 12 pressure increase in sealed vials fed with non-gaseous substrates as acetate, propionate, 13 butyrate was monitored.…”

Section: Specific Methanogenic Activity Test 10mentioning

confidence: 99%

Chronic impact of tetracycline on the biodegradation of an organic substrate mixture under anaerobic conditions

et al. 2013

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24The study evaluates the chronic impact of the antibiotic tetracycline on the biodegradation of 25 organic substrate under anaerobic conditions. The experiments involved an anaerobic 26 M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT2 sequencing batch reactor fed with a synthetic substrate mixture including glucose, starch and 1 volatile fatty acids, and operated in a sequence of different phases with gradually increasing 2 tetracycline doses of 1.65 -8.5 mg/L, for more than five months. Tetracycline exerted a 3 terminal/lethal effect at 8.5 mg/L on the microbial community under anaerobic conditions, 4 which caused the inhibition of substrate/COD utilization and biogas generation and leading to 5 a total collapse of the reactor. The microbial activity could not be recovered and re-started 6 within a period of more than 10 days, even after stopping tetracycline dosing. At lower doses, 7 substrate utilization was not affected but a reduction of 10-20% was observed in the 8 biogas/methane generation, suggesting that substrate utilization of tetracycline to the 9 biomass was limiting their bioavailability. During the experiments, tetracycline was partially 10 removed either through biodegradation or conversion into its by-products. The adverse long-11 term impact was quite variable for fermenting heterotrophic and methanogenic fractions of 12 the microbial community based on changes inflicted on the composition of remaining/residual 13 organic substrate. 14 15 16

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“…Batch specific methanogenic activity (SMA) assays were performed using the seed inoculum and reactor biomass samples from day 673 and day 1243 according to the protocols developed by Colleran et al (1992) and Coates et al (1996), and as described in detail in McKeown et al (2009). At 15 months after the conclusion of the trial, the hydrogenotrophic methanogenic activity of the reactor biomass-which had been stored at 4 1C without receiving any additional substratewas determined.…”

Section: Physiological Assaysmentioning

confidence: 99%

Psychrophilic methanogenic community development during long-term cultivation of anaerobic granular biofilms

et al. 2009

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Granular biomass was temporally sampled from a cold (4-15 1C) anaerobic bioreactor, which was inoculated with mesophilic biomass and used to treat industrial wastewater in a long-term (3.4 year) study. Data from 16S rRNA gene clone libraries, quantitative PCR and terminal restriction fragment length polymorphism analyses indicated that microbial community structure was dynamic, with shifts in the archaeal and bacterial communities' structures observed following start-up and during temperature decreases from 15 to 9.5 1C (phase 1). Specifically, the relative abundance of architecturally important Methanosaeta-like (acetoclastic) methanogens decreased, which was concomitant with granule disintegration and the development of a putatively psychrophilic hydrogenotrophic methanogenic community. Genetic fingerprinting suggested the development of a psychroactive methanogenic community between 4 and 10 1C (phase 2), which was dominated by acetogenic bacteria and Methanocorpusculum-like (hydrogenotrophic) methanogens. High levels of Methanosaeta-like acetoclastic methanogens and granular biofilm integrity were maintained during phase 2. Overall, decreasing temperature resulted in distinctly altered microbial community structure during phase 1, and the development of a less dynamic psychroactive methanogenic consortium during phase 2. Moreover, psychrophilic H 2 -oxidizing methanogens emerged as important members of the psychroactive consortia after 41200 days of low-temperature cultivation. The data suggest that prolonged psychrophilic cultivation of mesophilic biomass can establish a well-functioning psychroactive methanogenic consortium, thus highlighting the potential of low-temperature anaerobic digestion technology.

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Use of Methanogenic Activity Tests to Characterize Anaerobic Sludges, Screen for Anaerobic Biodegradability and Determine Toxicity Thresholds against Individual Anaerobic Trophic Groups and Species

Cited by 136 publications

References 0 publications

Recovery of Protein Concentrates From Microalgal Biomass Grown in Manure for Fish Feed and Valorization of the By-Products Through Anaerobic Digestion

Recovery of Protein Concentrates From Microalgal Biomass Grown in Manure for Fish Feed and Valorization of the By-Products Through Anaerobic Digestion

Chronic impact of tetracycline on the biodegradation of an organic substrate mixture under anaerobic conditions

Psychrophilic methanogenic community development during long-term cultivation of anaerobic granular biofilms

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