Use of micellar mobile phases for the chromatographic determination of melamine in dietetic supplements

Abstract

Melamine is a nitrogen-rich industrial chemical which is occasionally used to increase the apparent protein content of different products destined for human and animal consumption. In this work, a liquid chromatographic procedure that uses micellar mobile phases of sodium dodecyl sulfate (SDS) buffered at pH 3, a C18 column and UV detection is reported for the determination of melamine in dietetic supplements. Samples were reconstituted with a SDS solution and were directly injected, thus avoiding long extraction and experimental procedures. Melamine was eluted in less than 10 min with no interference by other compounds of the matrices. The optimum mobile phase composition was taken by a chemometrical approach that considers the retention factor, efficiency and peak shape. Validation was performed following the indications of the European Commission (Decision 2002/657/EC). The following parameters were considered: linearity (0.02-100 mg mL À1 ; R 2 ¼ 0.9996), intra-and inter-day precisions (<12.4%), accuracy (90.0-101.3%), and robustness (less than 9.8% and 5.1%, for retention time and peak area, respectively). The limits of detection and quantification were 9 and 20 ng mL À1 , respectively. Recoveries for several spiked samples were in the 85.8-114.3% range. These results indicate that the proposed methodology is useful for routine analysis of control quality of infant formula and adult dietetic supplements.

Application of Micellar Liquid Chromatography for The Determination of Antitumoral and Antiretroviral Drugs in Plasma

“…This modeling allows the prediction of the retention factor, efficiency, asymmetry and resolution, as well as the drawing of simulated chromatograms. The reliability of these simulations, checked for different groups of compounds by comparing experimental and predicted retention factors [23,24,38,40,43,45,46], is confirmed by their low errors (<5%). The optimal mobile phase was selected on the basis of maximum resolution/minimum analysis times.…”

Micellar high performance liquid chromatographic determination of flunixin meglumine in bulk, pharmaceutical dosage forms, bovine liver and kidney

“…Micellar mobile phases usually need less quantity of organic modifier and generate less amount of toxic waste in comparison to aqueous-organic solvents, so that they are less toxic, non-inflammable, biodegradable and relatively inexpensive [18]. MLC has proved to be a useful technique in the determination of diverse [19][20][21][22][23][24], including food samples [25][26][27][28].…”

Micellar Liquid Chromatography from Green Analysis Perspective

“…Another interesting application of MLC is the analysis of food samples [81][82][83][84][85][86][87][88][89][90][91][92][93][94][95]. This includes the determination of several compounds in chicken muscles and liver [81][82][83]95]; bovine meat, liver, and kidney [81,82,84,95]; swine kidney [89]; eggs [81,83,93,95]; milk [82,87,93,95]; baby food [82,83,95]; honey [92]; fish sauce [91,94]; feeding staff [86,90]; olive extract samples [85]; and dietetic supplements [88].…”

“…This includes the determination of several compounds in chicken muscles and liver [81][82][83]95]; bovine meat, liver, and kidney [81,82,84,95]; swine kidney [89]; eggs [81,83,93,95]; milk [82,87,93,95]; baby food [82,83,95]; honey [92]; fish sauce [91,94]; feeding staff [86,90]; olive extract samples [85]; and dietetic supplements [88]. MLC has been shown to be a very efficient technique for food analysis, avoiding long and tedious extractions of such complex matrices while providing high percentage recoveries with high accuracy and precision.…”