Use of micro-lightguide spectrophotometry for evaluation of microcirculation in the small and large intestines of horses without gastrointestinal disease

Abstract

The micro-lightguide spectrophotometry system was easy to use on the small and large intestines of horses and provided rapid evaluation of the microcirculation. Results indicated that measurements should be performed with room light only and intestinal motion should be minimized.

Correlation between capillary oxygen saturation and small intestinal wall thickness in the equine colic patient

“…Despite the above-mentioned difficulties, the results of the two methods were as follows: the oxygen saturation at MP1 was 40.5% (±25.9% MD) and therefore clearly lower than the previously reported value (Reichert and others 2014) of 81.04% (±12.62% SD) in healthy horses. This considerably lower level of oxygen saturation in the small intestine can be traced back to the strangulation obstruction and therefore the resulting extravasation of vessels.…”

“…The high variance is also traceable to various causes of ischaemia mentioned above. The parameter FLOW at MP2–MP6 was unremarkable according to the literature (Reichert and others 2014). Values ranged from 205.5 to 294 a.u., whereas in healthy horses it ranged from 216 to 246 a.u.…”

“…The relative amount of haemoglobin was clearly higher at each MP than in healthy horses (Reichert and others 2014). At MP1 and MP2, this is a sign of venous stasis due to ileus.…”

“…Methods for detecting ischaemic injury include a viability score (Freeman and others 2014), Doppler ultrasound, fluorescein fluorescence (Freeman and others 1988) and pulse oximetry (Schmotzer and others 1991). It has been proven (Reichert and others 2014) that spectrophotometry is suitable for the evaluation of microcirculation of the small intestine. The oxygen to see (O2C) works with a combination of laser Doppler spectroscopy and white light spectroscopy.…”

“…The blood flow is calculated out of the laser-detected movements of the erythrocytes and the oxygen saturation and the relative amount of haemoglobin are determined by the oxygen-dependent change in blood colour (KrugKrug 2007). According to the literature (Reichert and others 2014), oxygen saturation of healthy bowel is 81.04% (±12.62%) in the small intestine and 83.44% (±11.79%) in the large intestine. In the jejunum, the relative amount of haemoglobin is 68.41 a.u.…”