Use of Microsatellites for Parentage and Kinship Analyses in Animals

Webster, Michael S.; Reichart, Letitia M.

doi:10.1016/s0076-6879(05)95014-3

Cited by 38 publications

(32 citation statements)

References 37 publications

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“…2008b; Wagner et al. 2006; Webster & Reichart 2005): null alleles fail to amplify during PCR, often because of mutations within the primer site. Results obtained in two independent estimates of null homozygote frequency are consistent.…”

Section: Discussionmentioning

confidence: 99%

Contrasting genetic structures of two parasitic nematodes, determined on the basis of neutral microsatellite markers and selected anthelmintic resistance markers

et al. 2009

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For the first time, the neutral genetic relatedness of natural populations of Trichostrongylid nematodes was investigated in relation to polymorphism of the beta-tubulin gene, which is selected for anthelminthic treatments. The aim of the study was to assess the contribution of several evolutionary processes: migration and genetic drift by neutral genetic markers and selection by anthelminthic treatments on the presence of resistance alleles at beta-tubulin. We studied two nematode species (Teladorsagia circumcincta and Haemonchus contortus) common in temperate climatic zones; these species are characterized by contrasting life history traits. We studied 10 isolated populations of goat nematode parasites: no infected adult goat had been exchanged after the herds were established. Beta-tubulin polymorphism was similar in these two species. One and two beta-tubulin alleles from T. circumcincta and H. contortus respectively were shared by several populations. Most of the beta-tubulin alleles were 'private' alleles. No recombination between alleles was detected in BZ-resistant alleles from T. circumcincta and H. contortus. The T. circumcincta populations have not diverged much since their isolation (F(ST) <0.08), whereas H. contortus displayed marked local genetic differentiation (F(ST) ranging from 0.08 to 0.18). These findings suggest that there are severe bottlenecks in the H. contortus populations, possibly because of their reduced abundance during unfavourable periods and their high reproductive rate, which allows the species to persist even after severe population reduction. Overall, the data reported contradict the hypothesis of the origin of beta-tubulin resistance alleles in these populations from a single mutational event, but two other hypotheses (recurrent mutation generating new alleles in isolated populations and the introduction of existing alleles) emerge as equally likely.

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Section: Discussionmentioning

confidence: 99%

Contrasting genetic structures of two parasitic nematodes, determined on the basis of neutral microsatellite markers and selected anthelmintic resistance markers

et al. 2009

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“…While the characteristics of microsatellites make them well suited for parentage studies (Webster and Reichart 2005), regional implementation of PBT in the Columbia River basin required multiple independent laboratories to annually genotype tens of thousands of samples at the same suite of loci and necessitated the use of a different class of marker. The first was the identification of molecular markers with enough genetic variation to provide accurate parentage assignment even when thousands of potential parents from different populations across multiple spawn years were analyzed simultaneously.…”

Section: Development Of Pbt-tools and Techniques Molecular Markersmentioning

confidence: 99%

Parentage‐Based Tagging: Reviewing the Implementation of a New Tool for an Old Problem

et al. 2019

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Parentage‐based tagging (PBT), an innovative and large‐scale application of genetic parentage assignments, is transforming how fisheries managers determine the age and origin of sampled fish. PBT is an efficient alternative for mass tagging and has been widely implemented in the Pacific Northwest. While still an emerging technology, PBT is being used to provide information to managers in state, federal, and tribal agencies on the harvest, research, and conservation of Chinook Salmon Oncorhynchus tshawytscha and steelhead O. mykiss in this region. We review the development of PBT in the Pacific Northwest focusing on the technical and logistical challenges for implementing a regional PBT program. We also showcase recent results and review management efforts that made use of PBT‐derived data.

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“…Microsatellite DNA markers are among the most likely to conform to the assumption of neutrality and have proven to be powerful in differentiating geographically isolated populations and sibling species and subspecies [30]. The qualities of microsatellites make them very useful as genetic markers for studies of population differentiation and stock identification [35, 36], in kinship and parentage exclusion [37, 38] and in genome mapping [39]. Microsatellites are also being used as genetic markers for identification of population structure, genome mapping, pedigree analysis, and to resolve taxonomic ambiguities in many other animals besides fishes [40–49].…”

Section: Application Of Microsatellites In Population Structure Anmentioning

confidence: 99%

Application of Microsatellite Markers in Conservation Genetics and Fisheries Management: Recent Advances in Population Structure Analysis and Conservation Strategies

Abdul-Muneer

2014

Genetics Research International

166

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Microsatellites are the most popular and versatile genetic marker with myriads of applications in population genetics, conservation biology, and evolutionary biology. These are the arrays of DNA sequences, consisting of tandemly repeating mono-, di-, tri-, and tetranucleotide units, which are distributed throughout the genomes of most eukaryotic species. Microsatellites are codominant in nature, highly polymorphic, easily typed, and Mendelian inherited, all properties which make them very suitable for the study of population structure and pedigree analysis and capable of detecting differences among closely related species. PCR for microsatellites can be automated for identifying simple sequence repeat polymorphism. Small amount of blood samples or alcohol preserved tissue is adequate for analyzing them. Most of the microsatellites are noncoding, and therefore variations are independent of natural selection. These properties make microsatellites ideal genetic markers for conservation genetics and fisheries management. This review addresses the applications of microsatellite markers in conservation genetics and recent advances in population structure analysis in the context of fisheries management.

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Use of Microsatellites for Parentage and Kinship Analyses in Animals

Cited by 38 publications

References 37 publications

Contrasting genetic structures of two parasitic nematodes, determined on the basis of neutral microsatellite markers and selected anthelmintic resistance markers

Contrasting genetic structures of two parasitic nematodes, determined on the basis of neutral microsatellite markers and selected anthelmintic resistance markers

Parentage‐Based Tagging: Reviewing the Implementation of a New Tool for an Old Problem

Application of Microsatellite Markers in Conservation Genetics and Fisheries Management: Recent Advances in Population Structure Analysis and Conservation Strategies

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