1Milk produced by the grazing system, referred to as "grazing milk" contains many 2 components required for human health. The milk fatty acid (FA) profile is strongly 3 associated with the diet on the farms. In the present study, based on the FA profile 4 of farmer's bulk milk, we determined how to discriminate between milk produced 5 on grazing and on a confinement system. A field survey was conducted four times were collected and the FA profile of milk was measured. Milk produced during the 9 grazing season contained less C16:0 and cis-9 C16:0, and more C18:0, cis-9 C18:1, 10 trans-11 C18:1, cis-9,12 C18:2, cis-9,trans-11 C18:2 and cis-9,12,15 C18:3 than milk 11 produced during the confinement season. Discrimination analysis using 16 FA 12 revealed that almost all milk samples were discriminated correctly (confinement 13 season: 90% correct and 10% borderline, grazing season: 88% correct, 9% 14 borderline and 3% incorrect). For farmers that were categorized incorrectly and 15 were considered borderline in the grazing season, the dependency on pasture was 16 low compared with that for farmers discriminated correctly. Therefore, to claim 17 "grazing milk", a high dependency on pasture is required for grazing dairy farmers.