Use of mitochondrial and ribosomal DNA polymorphisms to classify clinical and soil isolates of Histoplasma capsulatum

Spitzer, Eric D.; Lasker, Brent A.; Travis, S J; Kobayashi, George S.; Medoff, Gerald

doi:10.1128/iai.57.5.1409-1412.1989

Cited by 92 publications

(51 citation statements)

References 16 publications

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“…These large fragments have been widely presumed to approximate to mtDNA, and this was demonstrated by comparison with banding patterns obtained from purified mitochondrial DNA for a number of species (e.g. Spitzer et al, 1989;Varga et al, 1993;Freeman & Shabi, 1996). Although direct comparisons between the MspI and HaeIII total genomic digests and purified mitochondrial DNA have seldom been undertaken with basidiomycete fungi, it would seem reasonable to assume that the majority of the bands obtained in these profiles are of mitochondrial origin, as they are in ascomycetes and oomycetes (Marriot et al, 1984;Whittaker et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

“…Total DNA was extracted using an adaptation (Paterson & Bridge, 1994) of a cetyltrimethyl ammonium bromide (CTAB)-based method (Zolan & Pukilla, 1986). DNA (3-8g) was digested with '4-base cutter' restriction endonucleases HaeIII or MspI (Gibco BRL, UK) to produce a small number of high molecular weight fragments presumed to correspond to mitochondrial DNA (Marriot et al, 1984;Spitzer et al, 1989). Fragments were separated in 1% agarose gels by electrophoresis at 5 V cm ¹1 and stained with ethidium bromide (0·5 g ml ¹1 ).…”

Section: Mitochondrial Dna Rflpsmentioning

confidence: 99%

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Genetic diversity of Ganoderma in oil palm plantings

Miller

Holderness

Chung³

et al. 1999

Plant Pathology

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Isolates of Ganoderma spp. associated with basal stem rot of oil palm were obtained by sampling from two palm plantings in Malaysia. Members of a subset of these were compared using a combination of somatic incompatibility and molecular genetic analyses. Incompatibility interactions between heterokaryons in culture indicated that Ganoderma generally occurred as numerous distinct strains, with compatible strains usually confined to individual palms. Analysis of restriction fragment length polymorphisms (RFLPs) in presumptive mitochondrial DNA (mtDNA) revealed heterogeneity among isolates from neighbouring palms, and from within individual palms. The combination of mtDNA variability and somatic incompatibility suggests that Ganoderma BSR does not spread by direct root-toroot contact between palms. The results may indicate infection spread through basidiospore dispersal or through contact with a heterogeneous long-term residual inoculum in debris.

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Section: Discussionmentioning

confidence: 99%

Section: Mitochondrial Dna Rflpsmentioning

confidence: 99%

Genetic diversity of Ganoderma in oil palm plantings

Miller

Holderness

Chung³

et al. 1999

Plant Pathology

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“…Strain UCLA 531 was provided by Dexter H. Howard, University of California School of Medicine, Los Angeles, USA (Stone et al, 1990). These strains were isolated from around the world and have been classified by their mitochondrial DNA restriction endonuclease patterns (Vincent et al, 1986;Spitzer et al, 1989;Eissenberg et al, 1991). The parental strains are designated with an 'R' suffix to denote their rough colony morphology.…”

Section: Methodsmentioning

confidence: 99%

Histoplasma acquisition of calcium and expression of CBP1 during intracellular parasitism

Batanghari

Deepe

et al. 1998

Molecular Microbiology

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SummaryA highly adapted parasite of macrophages, the yeast phase of Histoplasma capsulatum, survives and proliferates within phagolysosomes, while the mycelial phase exists only as a saprophyte in the soil. We have shown previously that these two phases of Histoplasma differ in their calcium requirements for growth and in the production of a released calcium-binding protein (CBP). Cloning and sequencing the CBP1 gene revealed two introns, a putative signal peptide and potential calcium-binding sites. We also evaluated CBP1 expression by reverse transcription-polymerase chain reaction (RT-PCR) of yeasts grown in broth culture and within two host cell types, a macrophage-like cell line and respiratory epithelial cells. H. capsulatum yeasts expressed CBP1 in all of these settings. Splenocytes from mice immunized with H. capsulatum yeasts responded to purified CBP in proliferation assays, providing evidence for the production of CBP during the infection of mammalian hosts. In addition, after H. capsulatum yeasts were subjected to a calcium-free shock, exogenously added CBP allowed yeasts to incorporate more calcium than yeasts incubated without added CBP. These results suggest that CBP may function to provide yeasts with calcium when they are in a low-calcium environment, such as the phagolysosomal compartment within macrophages.

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“…In the past years, H. capsulatum isolates from different geographic origins have been classified by their DNA polymorphism using molecular assays. These assays have revealed variation in genetic polymorphism depending on fungal sources or origins [21][22][23][24][25]. Diversity in population structures of H. capsulatum isolates has been suggested [26][27][28][29], including distinct fungal geographic populations [27].…”

Section: Introductionmentioning

confidence: 99%

Geographical distribution of genetic polymorphism of the pathogenHistoplasma capsulatumisolated from infected bats, captured in a central zone of Mexico

Taylor¹,

Chávez-Tapia²,

Rojas-Martínez³

et al. 2005

FEMS Immunology &Amp; Medical Microbiology

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Fourteen Histoplasma capsulatum isolates recovered from infected bats captured in Mexican caves and two human H. capsulatum reference strains were analyzed using random amplification of polymorphic DNA PCR-based and partial DNA sequences of four genes. Cluster analysis of random amplification of polymorphic DNA-patterns revealed differences for two H. capsulatum isolates of one migratory bat Tadarida brasiliensis. Three groups were identified by distance and maximum-parsimony analyses of arf, H-anti, ole, and tub1 H. capsulatum genes. Group I included most isolates from infected bats and one clinical strain from central Mexico; group II included the two isolates from T. brasiliensis; the human G-217B reference strain from USA formed an independent group III. Isolates from group II showed diversity in relation to groups I and III, suggesting a different H. capsulatum population.

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Use of mitochondrial and ribosomal DNA polymorphisms to classify clinical and soil isolates of Histoplasma capsulatum

Cited by 92 publications

References 16 publications

Genetic diversity of Ganoderma in oil palm plantings

Genetic diversity of Ganoderma in oil palm plantings

Histoplasma acquisition of calcium and expression of CBP1 during intracellular parasitism

Geographical distribution of genetic polymorphism of the pathogenHistoplasma capsulatumisolated from infected bats, captured in a central zone of Mexico

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