Use of modified McMaster method for the diagnosis of intestinal helminth infections and estimating parasitic egg load in human faecal samples in non-endemic areas

Bondarenko, Igor G.; Kinčeková, J.; Várady, Marián; Königová, Alžbeta; Kuchta, M.; Koňáková, G.

doi:10.2478/s11687-009-0012-0

Cited by 7 publications

(5 citation statements)

References 18 publications

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“…Its use has been described in detail in many diagnostics, both animal (Coles et al 1992;Karamon et al 2008;Morgan et al 2005;Nichols and Obendorf 1994;Pereckiene et al 2007;Rinaldi et al 2007;Ward et al 1997) and human (Bondarenko et al 2009;Flohr et al 2007;Stephenson et al 1989). Different McMaster method modifications use various weight of faeces examined, volumes and types of flotation solutions, sample dilutions, flotation times, applications of additional centrifugation, durations and speeds of centrifugation, numbers of sections of the McMaster slide counted and different coefficients for interpretation (Cringoli et al 2004;Karamon et al 2008;Pereckiene et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Which McMaster egg counting technique is the most reliable?

et al. 2011

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This study focuses on the comparison of three selected modifications of the McMaster counting technique, namely the McMaster method modified by Wetzel (W) and Zajíček (Z), as well as the concentration McMaster technique according to Roepstorff and Nansen (R&N). These modifications differ in the weights of faeces examined (W, 2 g/Z, 1 g/R&N, 4 g), flotation solutions (W, NaCl/Z, MgSO(4) + Na(2)S(2)O(3)/R&N, NaCl + glucose), centrifugation (W, none/Z, 2,000 RPM for 2 min and 2,000 RPM for 1 min/R&N, 1,200 RPM for 5 min), number of McMaster chambers investigated (W, 3/Z, 2/R&N, 2), and multiplication factors used (W, 67/Z, 33/R&N, 20). To investigate the sensitivity and reliability of these methods, nematode eggs (Teladorsagia circumcincta) were used. Parasite elements are distributed through negative binomial distribution in naturally infected host faeces, and the number of parasite elements in a given amount of faeces sample is unknown to man. Therefore, we decided to prepare the exact number of eggs which were added to the parasite negative faeces; the faecal sample was then investigated. From this perspective, this is the first time a comparison of the McMaster methods has been so accurately investigated. This approach allows us to evaluate the real sensitivity and reliability of the tested method. As the findings of this study indicate, the highest sensitivity and reliability were obtained using the Roepstorff and Nansen modification. This McMaster modification is able to detect 20 eggs per sample (in 70% of samples). Concentrations of 200 and 500 eggs can be found in almost 100% of samples. Moreover, this method is simple, cheap and fast. For these reasons, we can recommend this method for routine veterinary practice.

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Section: Introductionmentioning

confidence: 99%

Which McMaster egg counting technique is the most reliable?

et al. 2011

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“…McMaster method may be more successfully used (and with a better technical feasibility) than the stool thick smear for the detection and identification of geohelminth eggs. Moreover, additional benefits include the ability to perform further quantitative analyses of the sample when required, to determine the intensity of infection and, last but not least, to determine the efficacy of antiparasitic treatments (Bondarenko et al, 2009). In human parasitology, the McMaster technique was used in a study evaluating the efficacies of mebendazole against soil-transmitted helminth infections in Vietnam (Flohr et al 2007) and of albendazole against A. lumbricoides and T. trichiura in school-aged children in Kenya (Stephenson et al, 1989), the regions endemic for geohelminths.…”

Section: Introductionmentioning

confidence: 99%

Nematode infections in Slovak children hospitalised during 2008–2009

et al. 2010

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A study involved 1800 hospitalised children (age: 9 months to 16 years) examined by ovoscopic analyses and confirmed the occurrence of Ascaris lumbricoides and Trichuris trichiura in 46 patients (2.55 %). Of these, 30 patients had Ascaris infection, 13 were positive for both nematodes and 3 patients had Trichuris infection. The mean count of A. lumbricoides eggs in positive cases was 1050 eggs per gramme (EPG) in range 150 -4450 EPG. The mean count of T. trichiura eggs was 150 EPG (50 -250 EPG). The highest intensities of A. lumbricoides and T. trichiura infections occurred in children 3 -5 years of age living in poor hygienic conditions. Most common clinical conditions in all the patients included anaemia combined with complicated bronchopneumonia, colitis and gastritis. The strongest correlation between the parasite burden and selected laboratory test data (eosinophil count, haemoglobin, total serum iron) was found in children of 2 years of age (P < 0.05) and decreased with age.

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“…), and tapeworms (Taenia spp. ), and by egg counting using the modified McMaster technique (Bondarenko et al 2009) with a sensitivity of 15 eggs per gram (EPG) under a microscope; the results were expressed in terms of EPG of a 3-g fecal sample. We counted eggs using a counting apparatus (Fujihira, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

Dogs and Disease Threats to Giant Pandas in China

et al. 2019

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The potential threat of domestic dogs to wildlife habitat in China is not widely recognized, despite their large population, lack of regulations regarding their control, and threat they pose to native species. In a case study in 2017, we surveyed villages surrounding Liziping Nature Reserve, the primary site for the release of captive-born giant pandas (Ailuropoda melanoleuca) into the wild. We conducted surveys of dog owners to assess the population size, demographics, free-roaming status, and vaccination and sterilization history of their dogs. We collected blood and fecal samples to assess the prevalence of viral and parasite disease threats. At least 370 owned dogs lived near the core giant panda habitat; 64% were freeroaming, 21% had positive antibody titers for ≥1 of the 4 viruses we tested (canine distemper, parvovirus, rotavirus, rabies), and 67% were positive for gastrointestinal parasites. The high proportion of free-roaming dogs, uninhibited access to the reserve, and high prevalence of infectious diseases indicate that dogs pose a serious threat to wildlife within Liziping. The extent of this threat throughout the giant panda nature reserve network is unknown and should be assessed.

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Use of modified McMaster method for the diagnosis of intestinal helminth infections and estimating parasitic egg load in human faecal samples in non-endemic areas

Cited by 7 publications

References 18 publications

Which McMaster egg counting technique is the most reliable?

Which McMaster egg counting technique is the most reliable?

Nematode infections in Slovak children hospitalised during 2008–2009

Dogs and Disease Threats to Giant Pandas in China

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