Use of molecular probes to assess geographic distribution of Pfiesteria species.

Rublee, Parke A.; Kempton, Jason W.; Schaefer, Eric F.; Allen, Coy; Harris, Janera; Oldach, David; Bowers, Holly A.; Tengs, Torstein; Burkholder, JoAnn M.; Glasgow, Howard B.

doi:10.1289/ehp.01109s5765

Cited by 41 publications

(20 citation statements)

References 8 publications

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“…During late spring, summer and early fall of 1999 and 2000 abundance of Pfiesteria-like dinoflagellates was low (<49 cells ml À1 ) at all stations on the Pocomoke River (Stoecker and Gustafson, 2002;Stoecker and Gustafson, unpublished data). Samples collected in the lower Pocomoke River by Maryland DNR during 1999 and 2000 tested negative for Pfiesteria using PCR probes (Bowers and Oldach, personnel communication;Magnien, 2001b;MDNR, 2000MDNR, , 2001Rublee et al, 2001). Because of the low abundance of Pfiesteria, no attempts to correlate zoospore abundance with shear were made.…”

Section: Discussionmentioning

confidence: 99%

Effect of small-scale shear on grazing and growth of the dinoflagellate Pfiesteria piscicida

et al. 2006

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Section: Discussionmentioning

confidence: 99%

Effect of small-scale shear on grazing and growth of the dinoflagellate Pfiesteria piscicida

et al. 2006

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“…Although, PCR detection of microscopic organisms in environmental samples has previously been achieved (Morgan and Rogers 2001;Rublee et al 2001), it is considerably more difficult than genetic identification of pure samples (Godhe et al 2001). One of the problems with environmental samples can be a low concentration of target DNA.…”

Section: Discussionmentioning

confidence: 99%

Development and Evaluation of a PCR Based Assay for Detection of the Toxic Dinoflagellate, Gymnodinium catenatum(Graham) in Ballast Water and Environmental Samples

et al. 2005

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Gymnodinium catenatum is a bloom forming dinoflagellate that has been known to cause paralytic shellfish poisoning (PSP) in humans. It is being reported with increased frequency around the world, with ballast water transport implicated as a primary vector that may have contributed to its global spread. Major limitations to monitoring and management of its spread are the inability for early, rapid, and accurate detection of G. catenatum in plankton samples. This study explored the feasibility of developing a PCR-based method for specific detection of G. catenatum in cultures and heterogeneous ballast water and environmental samples. Sequence comparison of the large sub unit (LSU) ribosomal DNA locus of several strains and species of dinoflagellates allowed the design of G. catenatum specific PCR primers that are flanked by conserved regions. Assay specificity was validated through screening a range of dinoflagellate cultures, including the morphologically similar and taxonomically closely related species G. nolleri. Amplification of the diagnostic PCR product from all the strains of G. catenatum but not from other species of dinoflagellates tested imply the species specificity of the assay. Sensitivity of the assay to detect cysts in ballast water samples was established by simulated spiked experiments. The assay could detect G. catenatum in all 'blank' plankton samples that were spiked with five or more cysts. The assay was used to test environmental samples collected from the Derwent river estuary, Tasmania. Based on the results we conclude that the assay may be utilized in large scale screening of environmental and ballast water samples.

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“…[109], Gymnodinium [55], Pfiesteria spp. [136]) and fluorescent fragment detection (e.g., Pfiesteria [35]). Such PCRbased methods can be highly sensitive and have been successfully employed to amplify and detect single vegetative cells and cysts of harmful species [24].…”

Section: Detection Of Hab Speciesmentioning

confidence: 99%

Harmful algal blooms: causes, impacts and detection

Sellner

Doucette

Kirkpatrick

2003

Journal of Industrial Microbiology and Biotechnology

525

265

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Blooms of autotrophic algae and some heterotrophic protists are increasingly frequent in coastal waters around the world and are collectively grouped as harmful algal blooms (HABs). Blooms of these organisms are attributed to two primary factors: natural processes such as circulation, upwelling relaxation, and river flow; and, anthropogenic loadings leading to eutrophication. Unfortunately, the latter is commonly assumed to be the primary cause of all blooms, which is not the case in many instances. Moreover, although it is generally acknowledged that occurrences of these phenomena are increasing throughout the world's oceans, the reasons for this apparent increase remain debated and include not only eutrophication but increased observation efforts in coastal zones of the world. There is a rapidly advancing monitoring effort resulting from the perception of increased impacts from these HABs, manifested as expanding routine coastal monitoring programs, rapid development and deployment of new detection methods for individual species, toxins, and toxicities, and expansion of coastal modeling activities towards observational forecasts of bloom landfall and eventually bloom prediction. Together, these many efforts will provide resource managers with the tools needed to develop effective strategies for the management and mitigation of HABs and their frequently devastating impacts on the coastal environment.

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Use of molecular probes to assess geographic distribution of Pfiesteria species.

Cited by 41 publications

References 8 publications

Effect of small-scale shear on grazing and growth of the dinoflagellate Pfiesteria piscicida

Effect of small-scale shear on grazing and growth of the dinoflagellate Pfiesteria piscicida

Development and Evaluation of a PCR Based Assay for Detection of the Toxic Dinoflagellate, Gymnodinium catenatum(Graham) in Ballast Water and Environmental Samples

Harmful algal blooms: causes, impacts and detection

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