Use of molecular techniques to evaluate the survival of a microorganism injected into an aquifer

Thiem, Suzanne M.; Krumme, M.L.; Smith, Ronald L.; Tiedje, James M.

doi:10.1128/aem.60.4.1059-1067.1994

Cited by 57 publications

(10 citation statements)

References 27 publications

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“…In a previous study, the efficacy of ERIC-PCR has been reported to monitor the survivability of wild-type strain A. baumannii S30 in the field [1]. Thiem et al [30] have reported the use of a PCR-based molecular technique to evaluate survival of non-native Pseudomonas sp. strain B13 [30].…”

Section: Discussionmentioning

confidence: 99%

“…Thiem et al [30] have reported the use of a PCR-based molecular technique to evaluate survival of non-native Pseudomonas sp. strain B13 [30]. There are certain shortcomings in using PCR-based fingerprinting techniques with repeat element primers to monitor survivability of the introduced strains [3,8].…”

Section: Discussionmentioning

confidence: 99%

“…in many bacterial strains like Pseudomonas sp. [7,16,30,31]. Work has not been attempted so far on the incorporation of lux gene in Acinetobacter sp.…”

Section: Discussionmentioning

confidence: 99%

“…It has been suggested in previous reports that foreign gene expression may alter the host bacteria in subtle ways and may affect its population or ecological properties [7,30], thus further altering the functionality of the strain. However, there are reports of a better catabolic performance by a genetically modified Pseudomonad to degrade 3-chlorobenzoate (3-CB) and 4-methylbenzoate (4-MB) in a microcosm study [12].…”

Section: Discussionmentioning

confidence: 99%

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Crude oil degradation efficiency of a recombinant Acinetobacter baumannii strain and its survival in crude oil-contaminated soil microcosm

Mishra¹,

Sarma

Lal

2004

FEMS Microbiology Letters

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A hydrocarbon degrading Acinetobacter baumannii S30 strain, isolated from crude oil-contaminated soil, was inserted with the lux gene from the luciferase gene cassette luxCDABE. Soil microcosms were designed to study the degradation efficacy for total petroleum hydrocarbon (TPH) of crude oil by lux-tagged A. baumannii S30 pJES. Bioaugmentation of a TPH-contaminated microcosm with A baumannii S30 pJES showed that TPH levels were reduced from 89.3 to 53.9 g/kg soil in 90 days. Biodegradation of TPH by A baumannii S30 pJES was also monitored in shake flask conditions, which showed a reduction of initial TPH levels by over 50% at the end of 120 h. A lux-PCR-based approach along with the standard dilution plating with selective antibiotics was successfully utilized to monitor the survivability of the lux-tagged strain A. baumannii S30 pJES in soil microcosms and stability of the lux insert in the host strain A. baumannii S30. The selective plating technique indicated the population of A. baumannii S30 pJES to be 6.5+/-0.13 x 10(8) CFU/g at day zero (just after bioaugmentation) and 2.09+/-0.08 x 10(8) CFU/g of soil after 90 days of incubation. lux-PCR confirmed the stability of the insert in all the randomly selected colonies of A. baumannii strains from the antibiotic plates. The lux insert was stable after 50 generations in Luria Bertini broth and storage at -70 degrees C as glycerol stocks for over a year. These results revealed that the lux insert was stable and lux-tagged A. baumannii S30 strain could survive in a TPH-contaminated soil microcosm and could degrade TPH in the soil microcosm conditions. It can be used as an effective marker to monitor the survival of augmented strains at a bioremediation site.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…in many bacterial strains like Pseudomonas sp. [7,16,30,31]. Work has not been attempted so far on the incorporation of lux gene in Acinetobacter sp.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Crude oil degradation efficiency of a recombinant Acinetobacter baumannii strain and its survival in crude oil-contaminated soil microcosm

Mishra¹,

Sarma

Lal

2004

FEMS Microbiology Letters

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“…The polymerase chain reaction (PCR) has proved to be very useful because it allows a speci¢c segment of DNA to be ampli¢ed by a factor of 10 6 or more within hours, thus potentially permitting detection of cells which are present only in very low concentrations [7,25]. In addition, it has recently been demonstrated that the PCR technique can detect a low number of speci¢c bacteria against a large background of other prokaryotic and eukaryotic cells and of organic material which may be present in the samples [19,24,25]. These properties make PCR a particularly suitable method for analyzing environmental samples.…”

Section: Introductionmentioning

confidence: 99%

Environmental investigation of potentially pathogenic Vibrio parahaemolyticus in the Seto-Inland Sea, Japan

Alam

2002

FEMS Microbiology Letters

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Seawater and organic material (live and/or dead matter deposited on any substratum submersed in seawater) were collected during the cool weather season from a coast of the Seto-Inland Sea, Japan, and analyzed to determine Vibrio parahaemolyticus densities and the occurrence of pathogenic strains, defined as those possessing tdh and/or trh genes by the polymerase chain reaction (PCR), using isolated DNA from enrichment culture of the samples. About 95% of the samples were positive for V. parahaemolyticus (with densities of 3 to s 1400 cells per 100 ml water or 10 g organic samples) by the most-probable-number (MPN)-PCR technique with species-specific toxR primers, but only 40% were positive by the conventional MPN-culture technique (with densities ranging from 3 to 240 cells per 100 ml water or 10 g organics). Furthermore, the tdh and trh genes were positive in 55% and 20% of samples, respectively, by the MPN-PCR technique. No tdh and trh gene-positive strains were isolated by the conventional MPN-culture procedure. The difference in detection between the MPN-culture and the MPN-PCR techniques appeared to be significant and may be attributed to different detection sensitivities and other factors. ß

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Tracers in Groundwater: Use of Microorganisms and Microspheres

Harvey

Harms

2003

Encyclopedia of Environmental Microbiology

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Use of molecular techniques to evaluate the survival of a microorganism injected into an aquifer

Cited by 57 publications

References 27 publications

Crude oil degradation efficiency of a recombinant Acinetobacter baumannii strain and its survival in crude oil-contaminated soil microcosm

Crude oil degradation efficiency of a recombinant Acinetobacter baumannii strain and its survival in crude oil-contaminated soil microcosm

Environmental investigation of potentially pathogenic Vibrio parahaemolyticus in the Seto-Inland Sea, Japan

Tracers in Groundwater: Use of Microorganisms and Microspheres

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