Use of monoclonal antibodies for the diagnosis of cytomegalovirus infection by the detection of early antigen fluorescent foci (DEAFF) in cell culture

Stirk, P. R.; Griffiths, Paul

doi:10.1002/jmv.1890210405

Cited by 72 publications

(18 citation statements)

References 11 publications

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“…Amniotic fluid cultures, as well as an assay for early viral antigen detection (Stirk and Griffiths, 1987), were performed on all amniotic fluid samples. Fetal cord serum was assayed for the presence of CMV-specific IgM by an enzyme-linked immunosorbent assay (ELISA).…”

Section: Methodsmentioning

confidence: 99%

Prenatal diagnosis of cytomegalovirus (CMV) infection: A preliminary report

1993

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Eight patients were referred for prenatal diagnosis for suspected fetal cytomegalovirus infection (CMV): six for documented first-trimester infection and two for abnormal ultrasound evaluation suggestive of fetal infection. Three methods of diagnosis were employed: (1) amniotic fluid viral cultures and CMV-specific IgM in fetal serum; (2) amniotic fluid cultures and detection by polymerase chain reaction amplification of CMV-specific DNA in chorionic villi; and (3) detection of CMV-specific DNA in villus samples only. Amniotic fluid cultures detected all cases of infection, but CMV-specific IgM was not a reliable indicator of infection in any case. DNA analysis correlated well with both culture results and clinical outcome.

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Section: Methodsmentioning

confidence: 99%

Prenatal diagnosis of cytomegalovirus (CMV) infection: A preliminary report

1993

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“…Urine samples may be stored at 4°C for 7 days, with the isolation rate dropping to only 93%, whereas storage at room temperature or freezing decreases infectivity dramatically (263). In the 1980s, methods for rapid virus isolation were developed, based on the use of monoclonal antibodies to the HCMV major immediate-early protein p72 associated with low-speed centrifugation of clinical samples onto monolayers of human fibroblasts grown on coverslips inserted on the bottom of shell vials (6,93,98,242,270). The shell vial method was subsequently adapted to 96-well microtiter plates, where it showed a sensitivity of 94.5% and a specificity of 100% compared to standard virus isolation in a series of 1,676 newborn urine specimens (31).…”

Section: Diagnosis Of Congenital Infection In the Newbornmentioning

confidence: 99%

Diagnosis and Management of Human Cytomegalovirus Infection in the Mother, Fetus, and Newborn Infant

2002

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Human cytomegalovirus (HCMV) is the leading cause of congenital viral infection and mental retardation. HCMV infection, while causing asymptomatic infections in most immunocompetent subjects, can be transmitted during pregnancy from the mother with primary (and also recurrent) infection to the fetus. Hence, careful diagnosis of primary infection is required in the pregnant woman based on the most sensitive serologic assays (immunoglobulin M [IgM] and IgG avidity assays) and conventional virologic and molecular procedures for virus detection in blood. Maternal prognostic markers of fetal infection are still under investigation. If primary infection is diagnosed in a timely manner, prenatal diagnosis can be offered, including the search for virus and virus components in fetal blood and amniotic fluid, with fetal prognostic markers of HCMV disease still to be defined. However, the final step for definite diagnosis of congenital HCMV infection is detection of virus in the blood or urine in the first 1 to 2 weeks of life. To date, treatment of congenital infection with antiviral drugs is only palliative both prior to and after birth, whereas the only efficacious preventive measure seems to be the development of a safe and immunogenic vaccine, including recombinant, subunit, DNA, and peptide-based vaccines now under investigation. The following controversial issues are discussed in the light of the most recent advances in the field: the actual perception of the problem; universal serologic screening before pregnancy; the impact of correct counseling on decision making by the couple involved; the role of prenatal diagnosis in ascertaining transmission of virus to the fetus; the impact of preconceptional and periconceptional infections on the prevalence of congenital infection; and the prevalence of congenitally infected babies born to mothers who were immune prior to pregnancy compared to the number born to mothers undergoing primary infection during pregnancy

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“…BAL speci mens: BAL ef flu ent was di vided and sub mit ted for cul tures for bac te ria in 99 cases, fungi in 101, myco plasma in 95, chla my dia in 88, my co bac te rium in 100, vi ruses in 87 and cy tomega lovi rus (CMV) in 87. CMV early an ti gen was de tected by the shell vial tech nique (11)(12)(13)(14). BAL bac te rial cul tures were not quan ti fied for the num bers of bac te rial colo nies be cause this is not stan dard prac tice in the authors' labo ra tory.…”

Section: Pa Tients and Meth Odsmentioning

confidence: 99%

Fibreoptic Bronchoscopy in the Diagnosis of Pulmonary Disease in the Immunocompromised Host in Northern Alberta

Crocket¹,

Chaput²,

Lien³

1995

Canadian Journal of Infectious Diseases and Medical Microbiolog

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JA CROCKET, MR CHAPUT, DC LIEN. Fi bre op tic bron cho scopy in the di ag no sis of pul mo nary dis ease in the im mu no com pro mised host in north ern Al berta. Can J In fect Dis 1995;6(6):286-290. OB JEC TIVES:To de ter mine the di ag nos tic util ity of bron cho scopy in a popu la tion of im mu no com pro mised hosts in north ern Al berta. PA TIENTS AND METH ODS: Re sults from bron cho scopy in 86 im mu no com pro mised pa tients who un der went a to tal of 101 pro ce dures were ret ro spec tively re viewed. RE SULTS:The over all di ag nos tic yield was 57% with the high est yield in pa tients on im mu no sup pres sive drug ther apy (80%) and the low est yield in the group of bone mar row trans plant pa tients (27%). CON CLU SIONS: Bron cho scopy is a valu able tool for the evalua tion of pul mo nary dis ease in the im mu no com pro mised host. Over all di ag nos tic yield of 57% is com pa ra ble with that re ported in the lit era ture. Key Words: Bron choal veo lar lavage, Bron cho scopy, Im mu no com pro mised host, Pneu mo niaBronchoscopie au moyen de la fibre optique dans le diagnostic de la pneumopathie chez l'hôte immunodéficient dans le Nord de l'Alberta OB JEC TIFS : Dé ter mi ner l'u til ité di ag nos tique de la bron cho sco pie au près d'une popu la tion de su jets im mu nodéfi -cients, dans le Nord de l'Al berta. PA TIENTS ET MÉTH ODES : Les résul tats de la bron cho sco pie ef fec tuée chez 86 pa tients im mu nodéfi cients to tali sant 101 in ter ven tions ont été pas sés en re vue. RÉSUL TATS : Dans l'en sem ble, le ren de ment di ag nos tique à été de 57 %, le ren de ment étant le plus fort chez les patients sous traite ment médica men teux im mu no sup presseurs (80 %) et le plus fai ble, chez les pa tients ay ant subi une trans plan ta tion de moelle os seuse (27 %). CON CLU SIONS : La bron cho sco pie est utile pour éval uer la pneu mo pathie chez l'hôte im mu nodéfi cient. Le ren dement di ag nos tique global ob tenu (57 %) se com pare au ren de ment dont fait état la litté ra ture. 286CAN J IN FECT DIS VOL 6 NO 6 NO VEM BER/DE CEM BER 1995

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Use of monoclonal antibodies for the diagnosis of cytomegalovirus infection by the detection of early antigen fluorescent foci (DEAFF) in cell culture

Cited by 72 publications

References 11 publications

Prenatal diagnosis of cytomegalovirus (CMV) infection: A preliminary report

Prenatal diagnosis of cytomegalovirus (CMV) infection: A preliminary report

Diagnosis and Management of Human Cytomegalovirus Infection in the Mother, Fetus, and Newborn Infant

Fibreoptic Bronchoscopy in the Diagnosis of Pulmonary Disease in the Immunocompromised Host in Northern Alberta

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