1982
DOI: 10.1136/jcp.35.11.1253
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Use of monoclonal antibodies for the histopathological diagnosis of human malignancy

Abstract: SUMMARY This paper describes the use of a panel of seven monoclonal antibodies (selected so as to include reagents reactive with both epithelial and lymphoid cells) for distinguishing between anaplastic carcinoma and high grade lymphoma. Details are given of the immunohistological reactions of these antibodies against a wide range of both normal and malignant tissues and of a number of practical instances in which use of the antibody panel enabled a diagnosis to be made when routine histological examination ha… Show more

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Cited by 237 publications
(99 citation statements)
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“…29 As not all histiocytes necessarily stain with CD45, however, the relevance of this criterion has been debated. 74 While CD45 immunoreactivity may not be an absolute requirement with the aid of additional supportive studies such as electron microscopy, 42 differentiation from malignant fibrous histiocytoma (undifferentiated sarcoma) may be extremely difficult. These tumors are believed to arise from undifferentiated mesenchymal cells rather than histiocytes, and typically show pleomorphic and storiform growth patterns.…”
Section: Discussionmentioning
confidence: 99%
“…29 As not all histiocytes necessarily stain with CD45, however, the relevance of this criterion has been debated. 74 While CD45 immunoreactivity may not be an absolute requirement with the aid of additional supportive studies such as electron microscopy, 42 differentiation from malignant fibrous histiocytoma (undifferentiated sarcoma) may be extremely difficult. These tumors are believed to arise from undifferentiated mesenchymal cells rather than histiocytes, and typically show pleomorphic and storiform growth patterns.…”
Section: Discussionmentioning
confidence: 99%
“…These antibodies were derived from the Third and Fourth Workshops on Human Leucocyte Differentiation Antigens (Hogg & Horton, 1987;Knapp et al, 1989). Immunohistochemistry was performed using an indirect immunoperoxidase technique (Gatter et al, 1984).…”
Section: Methodsmentioning
confidence: 99%
“…These antibodies were derived from the Third and Fourth Workshops on Human Leucocyte Differentiation Antigens (Hogg & Horton, 1987;Knapp et al, 1989). Immunohistochemistry was performed using an indirect immunoperoxidase technique (Gatter et al, 1984).Preparation of isolated macrophages and macrophage polykaryons The remainder of the tissue was placed in normal saline and then transferred to Hanks BSS (Gibco). The tumour was cut into small pieces, digested for 2 h in Hanks containing 10 mg ml-' collagenase type II (Sigma).…”
mentioning
confidence: 99%
“…Table I. Immunohistochemistry was performed by immunoperoxidase or alkaline phosphatase anti-alkaline phosphatase (APAAP) procedures as previously described (Gatter et al, 1984). Cryostat sections were also stained for tartrate-resistant acid phosphatase using the method described by Evans et al (1979).…”
Section: Methodsmentioning
confidence: 99%