Use of Monoclonal Antibodies for Analyses of Coxiella burnetii Major Antigens

Hotta, Akitoyo; Zhang, Guo Quan; Andoh, Masako; Yamaguchi, Tsuyoshi; Fukushi, Hideto; Hirai, Katsuya

doi:10.1292/jvms.66.1289

Cited by 9 publications

(5 citation statements)

References 14 publications

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“…In most of these articles total protein extracts were analyzed, but few studied bacterial protein fractions enriched for outer membrane proteins (Papadioti et al, 2011;Jiao et al, 2014). These outer membrane proteins were found to be advantageous for the development of new vaccines due to their exposition to host immune cells (Hotta et al, 2004;Papadioti et al, 2011).…”

Section: Selection Of Immunoproteomic Publicationsmentioning

confidence: 99%

“…1). Because some authors have suggested that surface exposition might increase the chance for recognition by immune cells (Hotta et al, 2004;Papadioti et al, 2011), we assumed that outer membrane proteins would dominate. Surprisingly, the majority of the identified proteins are located in the cytoplasm (27%), followed by proteins associated with the inner (8%) or outer membrane (7%).…”

Section: Figmentioning

confidence: 99%

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Coxiella burnetii immunogenic proteins as a basis for new Q fever diagnostic and vaccine development

Gerlach¹,

Skultéty²,

Henning³

et al. 2017

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Summary. -Coxiella burnetii is the etiological agent of the zoonosis Q fever, which can cause an acute or a chronic, life-threatening disease in humans. It presents a highly stable cell form, which persists in the environment and is transmitted via contaminated aerosols. Ruminants are considered as the main reservoir for human infections but are usually asymptomatic. Subclinical infection in these animals and the occurrence of serologically negative shedders hamper the identification of infected animals with the currently used diagnostic techniques. This suboptimal sensitivity limits reliable identification of infected animals as well as the well-timed implementation of countermeasures. This review summarizes compounds, focusing on C. burnetii seroreactive proteins, which were discovered in recent immunoproteomic studies. We analyzed these proteins regarding their localization, function, frequency of citation, differences seen in various host species as well as sensitivity and specificity. Finally, proteins useful for the development of new diagnostic test systems as well as subunit vaccines were discussed.

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Section: Selection Of Immunoproteomic Publicationsmentioning

confidence: 99%

Section: Figmentioning

confidence: 99%

Coxiella burnetii immunogenic proteins as a basis for new Q fever diagnostic and vaccine development

Gerlach¹,

Skultéty²,

Henning³

et al. 2017

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“…Antigenic analysis of F. tularensis as well as other members of the genus is important because Francisella novicida and Francisella philomiragia have biochemical and genetic properties similar to those of F. tularensis (9), although they rarely cause tularemia-like diseases (13,22). Monoclonal antibodies (MAbs) are a useful tool for analyzing the antigenic properties of bacteria (15) Hybridoma clones secreting MAbs (M11D3, M11H7, M13B10, M14B11, M15C6, S11E7, and U22F2) were obtained by the fusion of mouse myeloma cells (P3-X63-Ag8.653) and spleen cells from BALB/c mice, which had been immunized with the formalin-inactivated F. tularensis GIEM Miura (Japanese) strain, the Schu (non-Japanese) strain, or the F. novicida U112 strain, as described elsewhere (14). Characteristics of the MAbs (Table 1) were based on MAbs obtained from hybridoma supernatant or mice ascitic fluids.…”

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confidence: 99%

Preparation of Monoclonal Antibodies for Detection and Identification of Francisella tularensis

Hotta

Uda

Fujita

et al. 2007

Clin Vaccine Immunol

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Monoclonal antibodies (MAbs) against Francisella tularensis were obtained. Three MAbs specifically reacted with F. tularensis, while four MAbs reacted with other members of the genus Francisella as well. Fluorescent isothiocyanate-conjugated MAbs unequivocally stained bacterial cells in specimens from experimentally infected mice. Two MAbs agglutinated F. tularensis antigen in the agglutination tests. These MAbs should improve methods for detection and identification of F. tularensis.

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“…We decided to focus on outer membrane proteins because they represent important virulence factors and are expected to stimulate specific immune responses. Additionally, their antigenic properties are poorly understood during human C. burnetii infection . Our team analyzed the sarcosyl‐insoluble and thus enriched in outer membrane proteins fraction of the two C. burnetii strains using doubled SDS‐PAGE combined with MS/MS analysis.…”

Section: Comparative Proteomics Studiesmentioning

confidence: 99%

The contribution of proteomics towards deciphering the enigma of Coxiella burnetii

Vranakis¹,

Papadioti

Tselentis³

et al. 2013

Proteomics Clinical Apps

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Coxiella burnetii, the causative agent of Q fever, is an intracellular bacterium and a potential weapon for bioterrorism. The widespread throughout the world, zoonosis is manifested clinically as a self-limited febrile illness, as pneumonia (acute Q fever) or as a chronic illness with endocarditis being its major complication. The recent Netherlands Q fever outbreak has driven the bacterium from a relatively cryptic, underappreciated, "niche" microorganism on the sideline of bacteriology, to one of possibly great impact on public health. Advances in the study of this microorganism proceeded slowly, primarily due to the, until recently, obligatory intracellular nature of the pathogen that in its virulent phase I must be manipulated under biosafety level-3 conditions. Proteomic studies, in particular, have generated a vast amount of information concerning several aspects of the bacterium such as virulence factors, detection/diagnostic and immunogenic biomarkers, inter-/intraspecies variation, resistance to antibiotics, and secreted effector proteins with significant clinical impact. The phenomenon observed following the genomics era, that of generation and accumulation of huge amount of data that ultimately end up unexploited on several databases, begins to emerge in the proteomics field as well. This review will focus on the advances in the field of C. burnetii proteomics through MS, attempting in parallel to utilize some of the proteomics findings by suggesting future directions for the improvement of Q fever diagnosis and therapy.

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Use of Monoclonal Antibodies for Analyses of Coxiella burnetii Major Antigens

Cited by 9 publications

References 14 publications

Coxiella burnetii immunogenic proteins as a basis for new Q fever diagnostic and vaccine development

Coxiella burnetii immunogenic proteins as a basis for new Q fever diagnostic and vaccine development

Preparation of Monoclonal Antibodies for Detection and Identification of Francisella tularensis

The contribution of proteomics towards deciphering the enigma of Coxiella burnetii

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