Use of monoclonal antibodies in diagnosis of paracoccidioidomycosis: new strategies for detection of circulating antigens

Gómez, Beatriz L.; Figueroa, Jose I.; Hamilton, Andrew J.; Ortiz, Blanca L.; Robledo, Mary Ann; Hay, Roderick J.; Restrepo, Ángela

doi:10.1128/jcm.35.12.3278-3283.1997

Cited by 58 publications

(31 citation statements)

References 37 publications

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“…Smear or culture can also be diagnostically useful, but P. brasiliensis grows extremely slowly. Serologic examination by immunodiffusion (Bueno et al, 1997;Gomez et al, 1997) or particularly the complement fixation test is useful mainly in epidemiological studies.…”

Section: Discussionmentioning

confidence: 99%

Oral paracoccidioidomycosis: a retrospective study of 62 Brazilian patients

Bicalho¹,

Santo²,

Santos³

2001

Oral Diseases

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OBJECTIVE: The purpose of this study was to evaluate retrospectively the profile of patients with oral paracoccidioidomycosis referred to two Dental Schools in Belo Horizonte (MG, Brazil) between 1955 and 1998. Despite the importance of the oral manifestations of this disease, few papers in the English literature have provided epidemiological data. SUBJECTS AND METHODS: The medical records of 62 patients presenting oral paracoccidioidomycosis were reviewed in detail. Patient age, gender, race, occupation, site of lesion and type of clinical manifestation of the disease were tabulated. RESULTS: There was a predominance of male patients (97%), with a male:female ratio of 30:1. The mean age was 40 years. Most of the patients were farm workers (53%). Some patients presented multiple oral lesions (19 cases, 30%). The fungal lesions were found principally in the alveolar process and gingiva, but were also seen on the palate, lip and buccal mucosa. All patients had chronic proliferative mulberry-like ulcerated oral lesions and the diagnosis was confirmed histologically. The clinical records did not contain notes about pulmonary involvement by the lesions. CONCLUSION: The purpose of this study was to describe the epidemiological profile of a specific population with the diagnosis of oral paracoccidioidomycois. The major goal is to establish a scientific basis for initiating educational programs for prevention and early diagnosis of oral paracoccidioidomycosis. Oral Diseases (2001) 7, 56-60

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Section: Discussionmentioning

confidence: 99%

Oral paracoccidioidomycosis: a retrospective study of 62 Brazilian patients

Bicalho¹,

Santo²,

Santos³

2001

Oral Diseases

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“…Finally, the definition of cure in PCM remains a matter of debate, and more prospective studies are needed. Other laboratory parameters, such as antigenaemia in urine or serum [16,58,59], may help to define the time points to change or stop therapy more accurately.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of tests for antibody response in the follow-up of patients with acute and chronic forms of paracoccidioidomycosis

Negro¹,

Pereira²,

Andrade

et al. 2000

Journal of Medical Microbiology

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Several serological tests have been used successfully in the diagnosis of paracoccidioidomycosis (PCM). In contrast, data about the use of these tests in the follow-up of PCM patients have been heterogeneous. In this study, serum samples from 43 PCM patients with different clinical forms were analysed by counter-immuno-electrophoresis (CIE), complement fixation (CF) and ELISA before treatment. With CIE and ELISA, the chronic unifocal form showed significantly lower antibody levels compared with chronic multifocal and acute forms. Acute form patients had significantly higher titres than patients with multifocal disease by CIE but not by ELISA. No significant differences were observed with CF. Twenty-seven of these patients were followed-up for 2 years and showed a decline in antibody levels by all three tests, paralleling clinical improvement. However, only patients with unifocal disease cleared their antibodies after 1 year of treatment as analysed by CF and ELISA and after 2 years by CIE, suggesting that these patients may need shorter courses of therapy. Patients with the other clinical form of the disease needed > or =2 years of therapy to clear their antibodies. Sera from a further five patients who presented with a relapse were analysed. At the time of relapse all showed increases in antibody levels by CIE and ELISA, but only three showed increases by CF tests. Therefore, CIE and ELISA demonstrated a better clinical correlation than CF, probably reflecting the fungal burden of PCM patients more accurately.

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“…Both antigens also could be detected in the CSF and BAL (58, 59) and in urine (105). Detection of a gp87‐kDa antigen also has been reported but would appear to be less useful because of cross‐reactions with other fungi and mycobacteria (106). However, whether these methods are available for routine clinical use is unknown.…”

Section: Paracoccidioidomycosismentioning

confidence: 99%

Antigen detection, serology, and molecular diagnosis of invasive mycoses in the immunocompromised host

Wheat¹

2006

Transplant Infectious Dis

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Understanding the uses and limitations of methods for rapid diagnosis of fungal disease is essential in order to diagnose and treat these infections early in their course. Antigen detection methods are useful for diagnosis of aspergillosis, cryptococcosis, histoplasmosis, blastomycosis, paracoccidioidomycosis, and penicilliosis marneffei. The accuracy of the beta-glucan assay for diagnosis of aspergillosis and candidiasis and its role in fungal diagnosis remains unclear, in part because the few published studies report widely varying specificity. Serologic tests for antibodies are also useful for diagnosis of histoplasmosis and coccidioidomycosis, but their sensitivity may be reduced by immunosuppression. While molecular diagnostic methods have been described and are available at some reference and university laboratories, their role in patient care remains uncertain, largely because of the lack of well-characterized assays and studies establishing their accuracy. Culture methods, although essential for establishing the diagnosis in some cases, have limitations for rapid diagnosis, namely insensitivity, need for invasive procedures, and delayed growth.

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Use of monoclonal antibodies in diagnosis of paracoccidioidomycosis: new strategies for detection of circulating antigens

Cited by 58 publications

References 37 publications

Oral paracoccidioidomycosis: a retrospective study of 62 Brazilian patients

Oral paracoccidioidomycosis: a retrospective study of 62 Brazilian patients

Evaluation of tests for antibody response in the follow-up of patients with acute and chronic forms of paracoccidioidomycosis

Antigen detection, serology, and molecular diagnosis of invasive mycoses in the immunocompromised host

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