Use of monoclonal antibody techniques to study the ontogeny of bovine anti-Mullerian hormone

Vigier, B.; Tran, Daniel; Buisson, F. Du Mesnil Du; Heyman, Yvan; Josso, Nathalie

doi:10.1530/jrf.0.0690207

Cited by 89 publications

(45 citation statements)

References 14 publications

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Section: Discussionsupporting

confidence: 93%

“…The AMH levels in all calves in the Castrated group were below the threshold for assay sensitivity (< 0.006 ng/ml). These findings are in agreement with previous observations of restricted expression of AMH by Sertoli cells in males [19]; moreover, the levels in blood decreased rapidly after castration [28].The plasma T concentrations in the present intact male calves, at 6 months of age, were greater than 1.0 ng/ml, except for the concentration in one calf (0.6 ng/ml), which was similar to that in normal bulls described in previous reports [27,29]. In general, plasma T concentrations decrease immediately after castration [29].…”

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confidence: 93%

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Anti-Müllerian Hormone (AMH) Profiles as a Novel Biomarker to Evaluate the Existence of a Functional Cryptorchid Testis in Japanese Black Calves

Kitahara

Ali

Sato

et al. 2012

Journal of Reproduction and Development

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Abstract. Anti-Müllerian hormone (AMH) and testosterone (T) profiles in blood were investigated before and after an hCG stimulation test to assess their sensitivity and specificity for the existence of a functional cryptorchid testis in Japanese Black calves. The hCG (3,000 IU) was administered on Day 0, and peripheral blood was collected on Days 0 (just before hCG injection), 5 and 7 in intact male calves (Intact; n=19), bilateral castrated calves (Castrated; n=17), unilateral cryptorchid calves, which abdominal testis could been extracted (Uni-crypto; n=9). Castration of a descended testis was carried in the Castrated and Uni-Crypto groups on Day -14. The AMH detectability and the optimum cut-off point for T levels using the receiver operating characteristic curve were verified to characterize the cryptorchid testis. AMH values on Day 0 were 21.1 ± 5.1 and 29.0 ± 7.5 ng/ml in the Intact and Uni-crypto groups, respectively (Mean ± SEM). AMH levels were under the detection limit in the Castrated group (i.e., < 0.006 ng/ml). T showed its peak levels on Day 5 in the Intact group (26.8 ± 4.2 ng/ml), while it remained low in the Castrated group (< 0.9 ng/ml) and did not show a significant difference in the Uni-crypto group. The detectable levels for AMH was 0.006 ng/ml, and the optimum cut-off point for T was 0.9 ng/ml; the sensitivity and specificity for evaluation of testicular descent into the scrotum were 1.0 for both the AMH and T levels. The detection rates in the Uni-crypto group using them were 1.0 and 0.57 for AMH on Day 0 and T on Days 5 or 7, respectively. In conclusion, plasma AMH profiles could be used as a novel biomarker to evaluate the existence of a functional cryptorchid testis in Japanese Black calves. Key words: Anti-Müllerian hormone (AMH), Bovine cryptorchidism, hCG stimulation test, Receiver operating characteristic, Testosterone (J. Reprod. Dev. 58: [310][311][312][313][314][315] 2012) C ryptorchidism is the failure of one or both testes to descend into the scrotum during the first trimester of pregnancy [1]. The plausible causes of cryptorchidism include insufficient secretion of insulin-like peptide 3 and/or testosterone (T) [1]. Cryptorchidism is most common in stallions, boars and some breeds of canine, but is rare in most other species [2,3]. The rate of morbidity with cryptorchidism in cattle is 0.2% [4]. The internal body temperature that surrounds the intra-abdominal retained testes associated with cryptorchidism is higher in comparison with that in the scrotum; this compromises spermatogenesis [5].Calves with a cryptorchid testis are commonly detected at castration [3]. If they have unilateral cryptorchid testes, the contralateral descended testes are extracted on the same day, and the cryptorchid testis is then usually looked for by laparotomy on another day. However, exteriorization of an abdominal testis is difficult [6].Fattening bulls with cryptorchidism in the later growing stages usually result in bulls with a stout body type that exhibit masculine characteristics in the ne...

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Section: Discussionsupporting

confidence: 93%

supporting

confidence: 93%

Anti-Müllerian Hormone (AMH) Profiles as a Novel Biomarker to Evaluate the Existence of a Functional Cryptorchid Testis in Japanese Black Calves

Kitahara

Ali

Sato

et al. 2012

Journal of Reproduction and Development

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“…This hormone has only been found in amniote vertebrates. In mammals, AMH expression starts in males at the onset of Sertoli cell differentiation and persists in adults late after regression of the Mü llerian ducts (Vigier et al, 1983). In females, ovarian AMH expression is delayed, starting around birth in granulosa cells of primary follicles (Bézard et al, 1987).…”

Section: Introductionmentioning

confidence: 99%

Different patterns of anti‐Müllerian hormone expression, as related to DMRT1, SF‐1, WT1, GATA‐4, Wnt‐4, and Lhx9 expression, in the chick differentiating gonads

Oréal

Mazaud²,

Picard

et al. 2002

Developmental Dynamics

131

106

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In mammals, anti-Mü llerian hormone (AMH) is produced by Sertoli cells from the onset of testicular differentiation and by granulosa cells after birth. In birds, AMH starts to be expressed in indifferent gonads of both sexes at a similar level and is later upregulated in males. We previously demonstrated that, unlike in mammals, the onset of AMH expression occurs in chick embryo in the absence of SOX9. We looked for potential factors that might be involved in regulating AMH expression at different stages of chick gonad differentiation by comparing its expression pattern in embryos and young chicken with that of DMRT1, SF-1, WT1, GATA-4, Wnt-4, and

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“…Immunoreactive AMH was detected in follicular fluid from ovarian follicles of adult cows (Vigier et al, 1984a;Necklaws et al, 1986). Ovarian AMH, purified from follicular fluid, exhibited bioactivity at a concentration of 4-6 x lop9 mol/l, similar to that required for bioactivity of testicular AMH, (Fig.…”

Section: Ovarian a M Hmentioning

confidence: 85%

“…Necklaws et al (1986) use a partially purified AMH preparation, Green-3 , to calibrate their assay, a fact which probably explains the 30-fold discrepancy between the results obtained by the two groups. The AMH concentration of serum of 2-week-old male calves is approximately 0.25 nmol/l (0.036 pg/ml), according to Vigier et al (1983) and 8 nmol/l (1.2 pg/ml) according to Necklaws et al (1986). Because of the zoospecificity of the monoclonal antibodies used (Legeai et al, 1986), only bovine, ovine and caprine AMH can be detected by this type of assay.…”

Section: Radioimmunoassaymentioning

confidence: 96%

Anti‐müllerian Hormone

Josso

1986

Clinical Endocrinology

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Use of monoclonal antibody techniques to study the ontogeny of bovine anti-Mullerian hormone

Abstract: Summary. Monoclonal antibodies against bovine anti-M\l=u"\llerian hormone (AMH) were used to study the hormone in cattle. Anti

Cited by 89 publications

References 14 publications

Anti-Müllerian Hormone (AMH) Profiles as a Novel Biomarker to Evaluate the Existence of a Functional Cryptorchid Testis in Japanese Black Calves

Anti-Müllerian Hormone (AMH) Profiles as a Novel Biomarker to Evaluate the Existence of a Functional Cryptorchid Testis in Japanese Black Calves

Different patterns of anti‐Müllerian hormone expression, as related to DMRT1, SF‐1, WT1, GATA‐4, Wnt‐4, and Lhx9 expression, in the chick differentiating gonads

Anti‐müllerian Hormone

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