Use of MTT colorimetric assay to measure cell activation

The Pied Crow (Corvus Albus) Is Insensitive to Diclofenac at Concentrations Present in Carrion

“…The RTE cell suspensions (200 ml at 10 5 cells/ml) were seeded into 96-well plates (Lu et al, 2004) and given 48 hr at 37 C in 5% CO 2 to establish before incubation with diclofenac (at 80, 40, 20, and 10 mM concentrations) for an additional 48 hr in the presence or absence of uric acid (0.18 mM). Cultures were then incubated with 0.44 mM MTT for 5 hr to assess cell viability (Gerlier and Thomasset, 1986). The degree of formazan formation was determined on a Varian spectrophotometer (Varian Medical Systems, Inc., Palo Alto, California, USA) at 570 nm (with a 1-cm path length).…”

Diclofenac toxicity in Gyps vulture is associated with decreased uric acid excretion and not renal portal vasoconstriction

“…Following the predetermined intervals of 12, 24 and 48 h, the cultures were incubated with MTT (0.44 umol) for 5 h (Lu et al, 2004, Orfila et al, 2000and Gerlier and Thomasset, 1986. The degree of formazan formation was determined by rinsing the wells with HBSS prior to the addition of 50 μl of DMSO and subsequent reading on a Varion spectrophotometer at 570 nm (1 cm path length).…”

Inorganic mercury modifies Ca2+ signals, triggers apoptosis and potentiates NMDA toxicity in cerebellar granule neurons

“…CGC survival was determined by the 3-(4,5-dimethyl-thiazole-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT) (Gerlier and Thomasset, 1986). Cells were cultured in 24-well-plates and the medium was replaced before each time point with a serum-free medium supplemented with 10% (v/v) stock solution (7.5 mg/ml) of MTT.…”