Use of nanosphere self-assembly to pattern nanoporous membranes for the study of extracellular vesicles

Mireles, Marcela; Soule, Cody W; Dehghani, Mehdi; Gaborski, Thomas R.

doi:10.1039/d0na00142b

Cited by 10 publications

(6 citation statements)

References 78 publications

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“…D. Kim et al, 2020;Mireles et al, 2020). These ultrafiltration-based chips seem to have higher recovery rates, however additional quality and purity validation studies on EVs purified using these chips should show their true potential.…”

Section: Techniques To Separate Evs For Functional Studiesmentioning

confidence: 99%

Extracellular Vesicle Heterogeneity and Its Impact for Regenerative Medicine Applications

et al. 2023

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Extracellular vesicles (EVs) are cell-derived membrane enclosed particles that are involved in physiological and pathological processes. EVs are increasingly being studied for therapeutic applications in the field of regenerative medicine. Therapeutic application of stem cell-derived EVs has shown great potential to stimulate tissue repair. However, the exact mechanisms through which they induce this effect has not been fully clarified. This may to a large extent be attributed to a lack of knowledge on EV heterogeneity. Recent studies suggest that EVs represent a heterogeneous population of vesicles with distinct functions. The heterogeneity of EVs can be attributed to differences in their biogenesis, and as such, they can be classified into distinct populations that can then be further subcategorized into various subpopulations. A better understanding of EV heterogeneity is crucial for elucidating their mechanisms of action in tissue regeneration. This review provides an overview of the latest insights on EV heterogeneity related to tissue repair, including the different characteristics that contribute to such heterogeneity, as well as the functional differences among EV subtypes. It also sheds light on the challenges that hinder clinical translation of EVs.Additionally, innovative EV isolation techniques for studying EV heterogeneity are discussed.Improved knowledge of active EV subtypes would promote the development of tailored EV therapies and aid researchers in the translation of EV-based therapeutics to the clinic.

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Section: Techniques To Separate Evs For Functional Studiesmentioning

confidence: 99%

Extracellular Vesicle Heterogeneity and Its Impact for Regenerative Medicine Applications

et al. 2023

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“…The membrane contains nanopockets of different sizes that can capture nanovesicles and protect them from fluid shear forces. 107 Pockets are arranged to capture and release small, medium, and large vesicles sequentially, thus combining purification and separation. Gaborski's group is now working to functionalize the nanopockets to enable them to distinguish between small EVs and similarly sized species like lipoproteins.…”

Section: A Nanomembrane-based Approach For Ev Purificationmentioning

confidence: 99%

“…The system incorporates concepts from chromatography and filtration to capture and protect nanovesicles. The membrane contains nanopockets of different sizes that can capture nanovesicles and protect them from fluid shear forces 107 . Pockets are arranged to capture and release small, medium, and large vesicles sequentially, thus combining purification and separation.…”

Section: Novel Technological Developmentsmentioning

confidence: 99%

Exosomes, microvesicles, and other extracellular vesicles—a Keystone Symposia report

Cable¹,

Witwer

Coffey

et al. 2023

Annals of the New York Academy of Sciences

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Extracellular vesicles (EVs) are small, lipid‐bilayer‐bound particles released by cells that can contain important bioactive molecules, including lipids, RNAs, and proteins. Once released in the extracellular environment, EVs can act as messengers locally as well as to distant tissues to coordinate tissue homeostasis and systemic responses. There is a growing interest in not only understanding the physiology of EVs as signaling particles but also leveraging them as minimally invasive diagnostic and prognostic biomarkers (e.g., they can be found in biofluids) and drug‐delivery vehicles. On October 30–November 2, 2022, researchers in the EV field convened for the Keystone symposium “Exosomes, Microvesicles, and Other Extracellular Vesicles” to discuss developing standardized language and methodology, new data on the basic biology of EVs and potential clinical utility, as well as novel technologies to isolate and characterize EVs.

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“…Over the past few decades, researchers developed a variety of methods for the preparation of M/N‐PMs. These methods include track etching, [ 16 ] phase inversion, [ 63 ] soft lithography, [ 64,65 ] micromolding in capillaries, [ 66 ] focus ion‐beam milling, [ 67,68 ] polymer casting, [ 69,70 ] electrospinning, [ 71 ] manual punching, [ 72 ] self‐assembled bead‐based lithography, [ 73,74 ] as well as phase‐separation microfabrication. [ 75,76 ] In the last 10 years, with the continuous research on the engineered membranes, several novel methods have been proposed for the preparation of M/N‐PMs.…”

Section: Fabrication Of M/n‐pmsmentioning

confidence: 99%

Advances in Micro/Nanoporous Membranes for Biomedical Engineering

Sun

Han

et al. 2021

Adv Healthcare Materials

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Porous membrane materials at the micro/nanoscale have exhibited practical and potential value for extensive biological and medical applications associated with filtration and isolation, cell separation and sorting, micro‐arrangement, in‐vitro tissue reconstruction, high‐throughput manipulation and analysis, and real‐time sensing. Herein, an overview of technological development of micro/nanoporous membranes (M/N‐PMs) is provided. Various membrane types and the progress documented in membrane fabrication techniques, including the electrochemical‐etching, laser‐based technology, microcontact printing, electron beam lithography, imprinting, capillary force lithography, spin coating, and microfluidic molding are described. Their key features, achievements, and limitations associated with micro/nanoporous membrane (M/N‐PM) preparation are discussed. The recently popularized applications of M/N‐PMs in biomedical engineering involving the separation of cells and biomolecules, bioparticle operations, biomimicking, micropatterning, bioassay, and biosensing are explored too. Finally, the challenges that need to be overcome for M/N‐PM fabrication and future applications are highlighted.

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Use of nanosphere self-assembly to pattern nanoporous membranes for the study of extracellular vesicles

Abstract: Nanospheres, similar in size and shape to extracellular vesicles, were used to pattern nanopores in an ultrathin membrane through a self-assembly process.

Cited by 10 publications

References 78 publications

Extracellular Vesicle Heterogeneity and Its Impact for Regenerative Medicine Applications

Extracellular Vesicle Heterogeneity and Its Impact for Regenerative Medicine Applications

Exosomes, microvesicles, and other extracellular vesicles—a Keystone Symposia report

Advances in Micro/Nanoporous Membranes for Biomedical Engineering

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