Use of New York City medium for improved recovery of Neisseria gonorrhoeae from clinical specimens

The lectin slide agglutination test for Neisseria gonorrhoeae has been modified and improved. Results show that wheat germ agglutinin and soybean lectin agglutinate 100% (193 of 193 tested) of clinical isolates of N. gonorrhoeae. Lectin-reactive meningococci can be readily identified by the hydrolysis of gammaglutamyl-3-naphthylamide. Branhamella catarrhalis, Neisseria lactamica, Neisseria sicca, Neisseria subflava, Neisseria perflava, and meningococcal serogroups A, B, C, X, Y, and Z do not interfere with the positive identification of N. gonorrhoeae. The frequently encountered problem of autoagglutination of members of the family Neisseriaceae may be circumvented by a short treatment of cellular suspensions with DNase. Based on agglutination assays, the enzyme treatment did not result in a loss of wheat germ agglutinin receptors from the bacteria. The lectin agglutination test, coupled with the gamma-glutamyl aminopeptidase assay, is proposed as a rapid and accurate means of identifying clinical isolates of gonococci.

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Diagnostic value of interactions between members of the family Neisseriaceae and lectins

Doyle¹,

Nedjat-Haiem²,

Keller³

et al. 1984

“…We modified the original formulation of NYC medium and found that the MNYC medium would support the rapid growth of stock strains of M. pneumoniae (9). In addition, we also found that modified formulations of NYC medium were superior to Martin-Lewis medium for the recovery of Neisseria gonorrhoeae from clinical specimens (8,10,11).…”

Section: Resultsmentioning

confidence: 95%

New York City medium for enhanced recovery of Mycoplasma pneumoniae from clinical specimens

Granato¹,

Poe²,

Weiner³

1983

Self Cite

Modified New York City (MNYC) medium and PPLO medium without methylene blue (PPLO agar) were compared for their ability to support the growth of Mycoplasma pneumoniae from clinical specimens. Pharyngeal specimens were collected from 1,070 college students who visited the Syracuse University Student Health Center. Of these patients, 623 were symptomatic for respiratory infection, and the remaining 447 were asymptomatic for respiratory illness. Throat swabs were inoculated into PPLO broths, and these broths were subcultured onto MNYC medium and PPLO agar after 3 and 14 days of incubation. A total of 222 (20.7%) clinical isolates of M. pneumoniae were recovered on these solid media, with the majority of the isolates (196) recovered from symptomatic patients. All isolates grew on MNYC medium, whereas five isolates failed to grow on PPLO agar. All isolates of M. pneumoniae recovered from symptomatic patients were detected on MNYC medium within 1 to 5 days of incubation, whereas 5 to 7 days of incubation were required before mycoplasmal growth was detected on PPLO agar. Over 86% of these mycoplasma isolates were detected on MNYC medium within 3 days of incubation and before the detection of any mycoplasmal growth on PPLO agar. A similar pattern of recovery times was observed for mycoplasmas isolated from asymptomatic patients. The results of this study have shown that MNYC medium is better than PPLO agar in supporting the rapid growth of M. pneumoniae from clinical specimens after 72-h blind subculture in PPLO glucose broth.

“…As reported, the use of this NYC medium resulted in enhanced recovery of N. gonorrhoeae isolated from clinical specimens as compared with the original NYC medium. Furthermore, in a recent evaluation comparing NYC medium with Martin-Lewis medium (11), we showed that the use of NYC medium results in a 13% overall increased recovery of gonococci isolated from clinical material.…”

mentioning

confidence: 85%

Primary isolation of Neisseria gonorrhoeae on hemoglobin-free New York City medium

Granato¹,

Schneible-Smith²,

Weiner³

1981

Self Cite

New York City medium and New York City medium without hemoglobin were comparatively evaluated for their ability to support the growth of Neisseria gonorrhoeae isolated from 1,010 clinical specimens. Although hemoglobin in the form of lysed horse erythrocytes stimulated gonococcal growth, the absence of this component from New York City medium did not have a detrimental effect on the recovery of gonococci isolated from clinical specimens. Both media were comparable in their ability to cultivate gonococci from clinical material, with a total of 187 gonococcal isolates being recovered on each of the media. The results of this study showed that the preparation of New York City medium can be facilitated and that its cost can perhaps be reduced by the elimination of the hemoglobin component from the formulation without adverse effect on the recovery of N. gonorrhoeae isolated from clinical specimens.