Use of non-thermal plasma pre-treatment to enhance antibiotic action against mature Pseudomonas aeruginosa biofilms

Paldrychová, Martina; Vaňková, Eva; Kašparová, Petra; Sembolová, Eliška; Maťátková, Olga; Masák, Jan; Scholtz, Vladimír; Julák, Jaroslav

doi:10.1007/s11274-020-02891-6

Cited by 11 publications

(16 citation statements)

References 50 publications

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“…Its antimicrobial activity and possible applications were comprehensively described in several reviews (Liao et al, 2017;Gupta and Ayan, 2019;Scholtz et al, 2021). We have previously verified great antibiofilm effect of NTP towards various species, especially P. aeruginosa (Paldrychováet al, 2019;Julaḱ et al, 2020;Paldrychováet al, 2020), and in continuation, the presented study observed whether NTP would affect virulence manifestation of P. aeruginosa (ability to re-form biofilm, produce Las-B elastase, proteases, haemolysins, lipases, gelatinase or pyocyanin) or its QS system. This study could contribute to the further spread of NTP as an efficient antimicrobial, antivirulence or anti-QS tool for the treatment of infectious diseases caused by P. aeruginosa.…”

Section: Introductionsupporting

confidence: 59%

Non-thermal plasma causes Pseudomonas aeruginosa biofilm release to planktonic form and inhibits production of Las-B elastase, protease and pyocyanin

Kašparová

Vaňková²,

Paldrychová³

et al. 2022

Front. Cell. Infect. Microbiol.

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The increasing risk of antibiotic failure in the treatment of Pseudomonas aeruginosa infections is largely related to the production of a wide range of virulence factors. The use of non-thermal plasma (NTP) is a promising alternative to antimicrobial treatment. Nevertheless, there is still a lack of knowledge about the effects of NTP on the virulence factors production. We evaluated the ability of four NTP-affected P. aeruginosa strains to re-form biofilm and produce Las-B elastase, proteases, lipases, haemolysins, gelatinase or pyocyanin. Highly strains-dependent inhibitory activity of NTP against extracellular virulence factors production was observed. Las-B elastase activity was reduced up to 82% after 15-min NTP treatment, protease activity and pyocyanin production by biofilm cells was completely inhibited after 60 min, in contrast to lipases and gelatinase production, which remained unchanged. However, for all strains tested, a notable reduction in biofilm re-development ability was depicted using spinning disc confocal microscopy. In addition, NTP exposure of mature biofilms caused disruption of biofilm cells and their dispersion into the environment, as shown by transmission electron microscopy. This appears to be a key step that could help overcome the high resistance of P. aeruginosa and its eventual elimination, for example in combination with antibiotics still highly effective against planktonic cells.

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Section: Introductionsupporting

confidence: 59%

Non-thermal plasma causes Pseudomonas aeruginosa biofilm release to planktonic form and inhibits production of Las-B elastase, protease and pyocyanin

Kašparová

Vaňková²,

Paldrychová³

et al. 2022

Front. Cell. Infect. Microbiol.

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“…All respiratory pathogens treated by NTP revealed complete inactivation within the tested time frame of exposure, thus meeting the FDA requirements for a decontamination technique (Ludwig-Begall et al, 2020). Our earlier data on NTP efficiency against P. aeruginosa growing in the form of resistant biofilms suggest that the technology is also efficient under more challenging conditions than those tested in the current study (Paldrychová et al, 2019;Vaňková et al, 2019;Paldrychová et al, 2020). The main advantages of the NTP technology include its extremely easy implementation requiring a simple plug-in device with a metal electrode placed within a shell that can be produced very inexpensively in any desired shape by 3D printing.…”

Section: Non-thermal Plasma Is a Potent Decontamination Technique Req...supporting

confidence: 76%

Decontamination of High-Efficiency Mask Filters From Respiratory Pathogens Including SARS-CoV-2 by Non-thermal Plasma

Obrova

Vaňková

Sláma

et al. 2022

Front. Bioeng. Biotechnol.

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The current pandemic resulted in a rapidly increasing demand for personal protective equipment (PPE) initially leading to severe shortages of these items. Hence, during an unexpected and fast virus spread, the possibility of reusing highly efficient protective equipment could provide a viable solution for keeping both healthcare professionals and the general public equipped and protected. This requires an efficient decontamination technique that preserves functionality of the sensitive materials used for PPE production. Non-thermal plasma (NTP) is a decontamination technique with documented efficiency against select bacterial and fungal pathogens combined with low damage to exposed materials. We have investigated NTP for decontamination of high-efficiency P3 R filters from viral respiratory pathogens in comparison to other commonly used techniques. We show that NTP treatment completely inactivates SARS-CoV-2 and three other common human respiratory viruses including Influenza A, Rhinovirus and Adenovirus, revealing an efficiency comparable to 90°C dry heat or UVC light. Unlike some of the tested techniques (e.g., autoclaving), NTP neither influenced the filtering efficiency nor the microstructure of the filter. We demonstrate that NTP is a powerful and economic technology for efficient decontamination of protective filters and other sensitive materials from different respiratory pathogens.

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“…When treated with 0.25 h NTP and then 8.5 mg/L GTM, P. aeruginosa ATCC 15442 mature biofilm was completely eliminated from the surface. Therefore, NTP can be used as a suitable antibiofilm agent in combination with antibiotics for the treatment of biofilm-associated infections caused by this pathogen ( Paldrychová et al, 2019 ; Paldrychova et al, 2020 ).…”

Section: Effect Of Superhydrophilicity Surface On Bacteriamentioning

confidence: 99%

Advances in the superhydrophilicity-modified titanium surfaces with antibacterial and pro-osteogenesis properties: A review

Shao

Wang

et al. 2022

Front. Bioeng. Biotechnol.

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In recent years, the rate of implant failure has been increasing. Microbial infection was the primary cause, and the main stages included bacterial adhesion, biofilm formation, and severe inhibition of implant osseointegration. Various biomaterials and their preparation methods have emerged to produce specific implants with antimicrobial or bactericidal properties to reduce implant infection caused by bacterial adhesion and effectively promote bone and implant integration. In this study, we reviewed the research progress of bone integration promotion and antibacterial action of superhydrophilic surfaces based on titanium alloys. First, the adverse reactions caused by bacterial adhesion to the implant surface, including infection and bone integration deficiency, are briefly introduced. Several commonly used antibacterial methods of titanium alloys are introduced. Secondly, we discuss the antibacterial properties of superhydrophilic surfaces based on ultraviolet photo-functionalization and plasma treatment, in contrast to the antibacterial principle of superhydrophobic surface morphology. Thirdly, the osteogenic effects of superhydrophilic surfaces are described, according to the processes of osseointegration: osteogenic immunity, angiogenesis, and osteogenic related cells. Finally, we discuss the challenges and prospects for the development of this superhydrophilic surface in clinical applications, as well as the prominent strategies and directions for future research.

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Use of non-thermal plasma pre-treatment to enhance antibiotic action against mature Pseudomonas aeruginosa biofilms

Cited by 11 publications

References 50 publications

Non-thermal plasma causes Pseudomonas aeruginosa biofilm release to planktonic form and inhibits production of Las-B elastase, protease and pyocyanin

Non-thermal plasma causes Pseudomonas aeruginosa biofilm release to planktonic form and inhibits production of Las-B elastase, protease and pyocyanin

Decontamination of High-Efficiency Mask Filters From Respiratory Pathogens Including SARS-CoV-2 by Non-thermal Plasma

Advances in the superhydrophilicity-modified titanium surfaces with antibacterial and pro-osteogenesis properties: A review

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