Use of nucleic acid amplification tests for the detection of Neisseria gonorrhoeae: survey of genitourinary medicine clinics in England

Abstract

bleeding disorder, were reported to experience hypermenorrhoea associated with ritonavir, but there is no such report for other PIs, although all PIs have similar pharmacologic effects. 4 As yet, there has not been enough experience with the newer PIs , such as ATV. As far as the patient is concerned, menorrhagia occurred after administration of ATV, recurred on rechallenge, stopped after discontinuation and the other drugs in her regimen had never caused her menorrhagia. This case report supports that ATV may cause menorrhagia. REFERENCES 1 Harlow SD, Schuman P, Cohen M, et al. Effect of HIV infection on menstrual cycle length. J Acquir Immune Defic Syndr Hum Retrovirol 2000;24:68-75 2 Minkoff HL, Eisenberger-Matityahu D, Feldman J, Burk R, Clarke L. Prevalence and incidence of gynecologic disorders among women infected with HIV. Am J Obstet Gynecol 1999;180:824-36 3 Wilde JT. Protese inhibitor therapy and bleeding. Haemophilia 2000;6:487-90 4 Nielsen H. Hypermenorrhoea associated with ritonavir. Lancet 1999;353:811-2

Validation of a laboratory-developed real-time PCR protocol for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine

“…The use of NAAT for the detection of C trachomatis is well established and is increasingly utilised for the detection of N gonorrhoeae , particularly as emphasis shifts towards the use of non-invasive specimen types 1 14 15. Several manufacturers now supply NAAT platforms that can screen urine samples for both C trachomatis and N gonorrhoeae in the same reaction.…”

Gonococcal NAATs: what is the current state of play in England and Wales?

“…This is a considerable increase on data generated in 2006 where 19% (29/152) of GU medicine clinics reported using molecular methods to detect GC. 12 This rapid increase in the use of GC NAATs in a relatively short period of time suggests that there is a present move in England and Wales towards molecular testing for GC. It will be essential for validation studies to be conducted prior to their introduction and that national guidelines and microbiology quality assurance schemes are updated to accommodate this change.…”

Diagnosis, management and prevalence estimation of gonorrhoea: influences of Aptima Combo 2 assay with alternative target confirmation

“…The aim of gonococcal antimicrobial resistance surveillance is to determine a suitable treatment, which cures a minimum of 95% of infections when given as a single dose, preferably on diagnosis or first presentation. 20 Microscopy in symptomatic patients facilitates rapid treatment; however, culture should be taken in GU medicine clinic patients to facilitate appropriate treatment in those who are symptomatic, positive on gram stain, a known contact of gonorrhoea, where treatment has failed and in patients who are NAAT positive and have not had treatment. The recent Gonococcal Resistance to Antimicrobials Surveillance Programme (GRASP annual report 2007) 21 showed resistance to antimicrobials much higher than found in our GU medicine clinic – it reported 28% resistance to ciprofloxacin, 24% to penicillin, 4% to azithromycin, 60% to tetracycline and for the first time ever found decreased susceptibility to cefixime in two isolates, none were reported to spectinomycin or ceftriaxone.…”