Use of Old Coconut Water with Various Skim Concentrations of Milk as a Diluent for Kampong Chicken Semen

Abstract

This study aims to determine the level of skim milk in the best coconut water that can maintain the quality of spermatozoa of native chickens during storage at 5oC. Semen was divided into five tubes (without diluents, diluents with skim milk levels 0%, 6%, 8% and 12, liquid Semen was then stored at 5oC. Observations of motility and viability were carried out at 0, 12, 24 hours, 36, and 48 hours. The results showed that the use of various diluents had a very significant effect (P <0.01) on the motility and viability of chicken spermatozoa at 12 hours of storage. The highest motility of spermatozoa was found in coconut milk diluent treatment with milk level skim 6%, and skim milk level 9% at 0 o'clock, 12 o'clock and 48 o'clock storage, while the highest motility at 24 o'clock and 36 o'clock was at treatment with skim milk level 6% Viability of spermatozoa at 0 o'clock does not differ between treatments but during storage, in general, the viability of spermatozoa can survive better in diluents with skim milk levels of 6% and 9% Conclusion of this study is coconut water Diluent with skim milk level of 6% and 9% can maintain the quality of chicken spermatozoa better during storage 48 hours at a temperature of 5oC.

The Effect of the Combination of Glucose Concentration with the Type of Extenders on the Quality of Native Rooster Spermatozoa during Storage

“…CE extenders induced better viability than LE and CS (Table 2); this might be due to the CE content having a higher glucose content so that it allows spermatozoa to survive longer. Viability of spermatozoa in previous studies using skim coconut milk 9% in 24-hour storage was 79.90% (Khaeruddin & Srimaharani, 2019) was lower than in this study which was 82.13%, probably due to the presence of glucose content in this study. The viability average at 24-hour storage in the present study was also higher than the report of Danang, Isnaini & Trisunuwati (2012) that found 55.4% in ringer's extender.…”

“…Whereas LE, LS 20 mM glucose, LS 50 mM glucose, CE 20 mM glucose, and CE 50 mM glucose extender were only able to maintain the progressive motility of spermatozoa for up to 48 hours. The spermatozoa motility in 80 mM glucose CS extender was 68.33 ± 5.77% at 48 hours storage, which was higher than previously obtained using coconut water + 9% skim milk extender which was 43.73% in the same type of chicken (Khaeruddin & Srimaharani, 2019).…”