Use of organism identification by 16S ribosomal RNA polymerase chain reaction to shorten antimicrobial length of therapy

Gilbert, Elise M.; Yucebay, Filiz; Malczynski, Mike; Smith, D.; Esterly, John S.; Qi, Chao; Postelnick, Michael; McLaughlin, Milena M.

doi:10.1016/j.diagmicrobio.2017.03.013

Cited by 4 publications

(2 citation statements)

References 13 publications

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“…Factors such as these could be taken into consideration by ordering clinicians aiming to increase the diagnostic yield. Although there have been some studies that reviewed the effect of the PCR result on antibiotic management [ 27 , 28 , 47 ], none of these assessed if there was any difference in antimicrobial management between patients testing PCR positive vs negative.…”

Section: Discussionmentioning

confidence: 99%

Microbial Identification Using DNA Target Amplification and Sequencing: Clinical Utility and Impact on Patient Management

Basein

Gardiner

Vazquez

et al. 2018

Open Forum Infectious Diseases

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Broad-range polymerase chain reaction (PCR) is increasingly used in patients with culture-negative infections; however, few studies have assessed the diagnostic utility of this test in this context. We performed a retrospective cohort study of patients who had clinical specimens sent for broad-range PCR, aiming to evaluate performance and determine impact on patient management. Organisms were identified in 21/71 samples. High numbers of polymorphonuclear leukocytes on Gram stain (odds ratio [OR], 4.17; P = .04) and acute inflammation on histopathology (OR, 5.69; P = .02) were significantly associated with a positive result. Management was altered in 18 patients, 11 with positive and 7 with negative results. Overall, broad-range PCR assay had the highest impact in patients with microscopic evidence of inflammation. Physicians ordering this complex, difficult to interpret, and expensive test should carefully consider all available clinical information on an individualized basis to optimize its performance.

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Section: Discussionmentioning

confidence: 99%

Microbial Identification Using DNA Target Amplification and Sequencing: Clinical Utility and Impact on Patient Management

Basein

Gardiner

Vazquez

et al. 2018

Open Forum Infectious Diseases

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“…Among 60 patients for whom 16S PCR was performed, O'Donnell et al observed deescalation of therapy in five patients and treatment discontinuation in two, resulting in 11.7% of treatment changes [12]. Gilbert et al reported that the length of therapy was shorter in case of negative 16S PCR and that antibiotics were discontinued more frequently than in case of positive 16S PCR [13].…”

Section: Introductionmentioning

confidence: 99%

16S rRNA PCR on clinical specimens: Impact on diagnosis and therapeutic management

Bador

Nicolas

Chapuis

et al. 2020

Médecine et Maladies Infectieuses

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The potential contribution of 16S ribosomal RNA polymerase chain reaction to antimicrobial stewardship in culture-negative infection

O’Donnell

Gaughan

Skally

et al. 2018

Journal of Hospital Infection

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Use of organism identification by 16S ribosomal RNA polymerase chain reaction to shorten antimicrobial length of therapy

Cited by 4 publications

References 13 publications

Microbial Identification Using DNA Target Amplification and Sequencing: Clinical Utility and Impact on Patient Management

Microbial Identification Using DNA Target Amplification and Sequencing: Clinical Utility and Impact on Patient Management

16S rRNA PCR on clinical specimens: Impact on diagnosis and therapeutic management

The potential contribution of 16S ribosomal RNA polymerase chain reaction to antimicrobial stewardship in culture-negative infection

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