Use of oxalic acid for screening intact sunflower plants for resistance to Sclerotinia in vitro

Vasić, Dragana M.; Škorić, Dragan; Taški, Ksenija J.; Stošić, Ljiljana

doi:10.2298/hel0236145v

Cited by 8 publications

(6 citation statements)

References 6 publications

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“…They were then rinsed three times with sterile distilled water and placed in 250 cm 3 Erlenmeyer flasks with 80 cm 3 of MS medium (Murashige and Skoog 1962) supplemented with 5 g dm -3 of sucrose, 6 g dm -3 of agar, and 0, 2, 3 and 4 mM of oxalic acid, pH 5.7. Seeds were further cultured under irradiance of 34 µmol m -2 s -1 , 16-h photoperiod, and temperature of 25 °C (Vasić et al 1999(Vasić et al , 2002. After two weeks of culture, samples of leaves and stems were taken for biochemical analyses.…”

Section: Methodsmentioning

confidence: 99%

Antioxidant Systems in Sunflower as Affected by Oxalic Acid

Malenčić¹,

Vasić²,

Popović³

et al. 2004

Biologia plant.

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Changes in antioxidant systems in sunflower (Helianthus annuus L.) inbred lines were studied in relation to different concentrations (0, 2, 3 and 4 mM) of oxalic acid. A great variability of the total superoxide dismutase (SOD) activity was observed in different genotypes after application of oxalic acid. Genotypes showing no change in SOD activity, as well as those with decreased and increased activities, have been found. The highest guaiacol peroxidase (GPX) activity was recorded in the leaves of Ha-26A genotype where the activity of SOD was low. In genotypes with increased GPX activity the malonyldialdehyde content was low. The increase in reduced glutathione content occured in almost all of the genotypes studied with the increase in oxalate concentration. Some genotypes such as PR-ST-3B, showed distinctive and combined activity of several antioxidant systems. Also, obtained results for the content of total phenols support the idea of using soluble phenols as molecular markers and confirm the hypothesis of the defensive role of these compounds in plants.

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Section: Methodsmentioning

confidence: 99%

Antioxidant Systems in Sunflower as Affected by Oxalic Acid

Malenčić¹,

Vasić²,

Popović³

et al. 2004

Biologia plant.

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“…These studies are based on a gene controlling the production of an enzyme oxalate oxidase (OXOX). Oxalate is a phytotoxin secreted by S. sclerotiorum (Vasic et al 2002). It weakens the plant tissue and crops with natural resistance to S. sclerotiorum such as wheat, barley, maize, or rice, producing OXOX, which breaks down and detoxifies the phytotoxin produced by S. sclerotiorum.…”

Section: Molecular Breeding and Transgenic Sunflower For Resistance To Sclerotinia Diseases In Sunflowermentioning

confidence: 99%

Diseases of Edible Oilseed Crops

Chattopadhyay¹,

Kolte²,

Waliyar³

2015

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AgriculturalSciences. He has more than 22 years of research experience in oilseed crop disease management and has significantly contributed in the areas of understanding the host-pathogen interactions, epi demiology, and integrated disease management in sunflower, safflower, sesame, peanut, and rape seed-mustard crops. He has published more than 70 peer-reviewed research papers and 4 books. Dr. Chattopadhyay has to his credit several academic awards and one patent. His significant research achievements include the development of Fusarium wilt-resistant genotypes in safflower, the iden tification of genotypes of Brassica juncea resistant to a mixture of isolates of Albugo candida, the development of a biocontrol agent formulation to reduce the use of fungicides in the management of major diseases of oilseeds (Brassica), and seed treatment (and foliar application) of aqueous bulb extract of Allium sativum for the management of major foliar diseases (Alternaria blight, white rust, powdery mildew, and Sclerotinia stem rot) of mustard. S.J. Kolte, professor of plant pathology (Retd.

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“…Oxalic acid concentration increases when tissues are damaged and this can be used as an indirect method for selecting on the basis of tolerance to disease (Vasic et al 2002).…”

Section: Disease Controlmentioning

confidence: 99%

Transgenic Sunflower

Cantamutto¹,

Poverene²

2010

Genetics, Genomics and Breeding of Sunflower

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At the beginning of the new millennium, sunflower relied on traditional non-GM varieties, which could be a possible disadvantage in relation to other oilseed crops. Different transformation protocols allowed obtaining desirable transgenic traits: the oxalate-oxidase expressing gene for fungal disease control, glyphosate tolerance by expressing Agrobacterium gene cp4, and the Bt toxin gene cry1 to control Lepidoptera. Ammonium absorption, other herbicide tolerance, Cry1 variants and the CpT1 gene for pest control are new targets. Quality can be improved through a modified protein and latex biosynthesis. The high cost of the GM crop approval process, increased by the probability of gene flow with wild relatives, is a constraint for the transgenic sunflower release. Strategies designed to minimize environmental risks and changes in consumer perception are needed before sunflower transgenic varieties become acceptable.

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Use of oxalic acid for screening intact sunflower plants for resistance to Sclerotinia in vitro

Cited by 8 publications

References 6 publications

Antioxidant Systems in Sunflower as Affected by Oxalic Acid

Antioxidant Systems in Sunflower as Affected by Oxalic Acid

Diseases of Edible Oilseed Crops

Transgenic Sunflower

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