Use of ozonated water as a new therapeutic approach to solve current concerns around antitumor treatment

Kuroda, Kohei; Yamashita, Masamiti; Murahata, Yusuke; Aso, Kazuo; Osaki, Tomohiro; Tsuka, Takeshi; Ito, Norihiko; Imagawa, Tomohiro; Okamoto, Yoshiharu

doi:10.3892/etm.2018.6415

Cited by 3 publications

(5 citation statements)

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“…Administration of ozonated water was shown to increase intratumoral blood perfusion and improve hypoxic conditions within tumor loci increasing the therapeutic outcome. Hydrogen peroxide could also increase apoptosis and decrease proliferation and multiphase cell cycle arrest in 4T1 cells to some extent [ 57 , 58 ]. A previous report applying oxygen nanobubble water on a similar mice model of breast cancer led to a 25% reduction in tumor volume [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

Immunotherapy Using Oxygenated Water and Tumor‐Derived Exosomes Potentiates Antitumor Immune Response and Attenuates Malignancy Tendency in Mice Model of Breast Cancer

Pakravan

Abbasi

Hassan

2021

Oxidative Medicine and Cellular Longevity

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Breast cancer is one of the most common type of tumor and the leading cause of death in the world’s female population. Various therapeutic approaches have been used to treat tumors but have not led to complete recovery and have even damaged normal cells in the body. Moreover, metastatic tumors such as breast cancer are much more resistant to treatment, and current treatments have not been very successful in treating them and remain a challenge. Therefore, new approaches should be applied to overcome this problem. Given the importance of hypoxia in tumor survival, we aimed to test the antitumor effects of oxygenated water to decrease hypoxia along with tumor-derived exosomes to target tumor. The purpose of administering oxygenated water and tumor exosomes was to reduce hypoxia and establish an effective immune response against tumor antigens, respectively. For this purpose, the breast cancer mice model was induced using the 4T1 cell line in Balb/c mice and treated with oxygenated water via an intratumoral (IT) and/or intraperitoneal (IP) route and/or exosome (TEX). Oxygenation via the IT+IP route was more efficient than oxygenation via the IT or IP route. The efficiency of oxygenation via the two routes along with TEX led to the best therapeutic outcome. Antitumor immune responses directed by TEX became optimized when systemic (IP) and local (IT) oxygenation was applied compared to administration of TEX alone. Results demonstrated a significant reduction in tumor size and the highest levels of IFN-γ and IL-17 and the lowest levels of IL-4 FoxP3, HIF-1α, VEGF, MMP-2, and MMP-9 in the IT+IP+TEX-treated group. Oxygenated water on the one hand could reduce tumor size, hypoxia, angiogenesis, and metastasis in the tumor microenvironment and on the other hand increases the effective immune response against the tumor systemically. This therapeutic approach is proposed as a new strategy for devising vaccines in a personalized approach.

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Section: Discussionmentioning

confidence: 99%

Immunotherapy Using Oxygenated Water and Tumor‐Derived Exosomes Potentiates Antitumor Immune Response and Attenuates Malignancy Tendency in Mice Model of Breast Cancer

Pakravan

Abbasi

Hassan

2021

Oxidative Medicine and Cellular Longevity

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“…Tendo em conta que o ser humano desde os tempos mais remotos sempre utilizou alguns meios e elementos para possibilitar a distinção e identificação dos indivíduos ou pessoas entre outros na esfera da sua convivência no meio social, variando de acordo com a época ou o lugar; logo, 5 Entre nós angolanos é bastante conhecido por B.I. 6 Vide: artigo 6º da Lei nº 4/09, de 30 de Junho. 7 ALVES, Maria da Piedade.…”

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“…In light of the above, the work aimed to demonstrate the potential of geotechnologies for monitoring forest cover in the Western Amazon, using the Enganado River microbasin as an example. The analysis of the microbrace was performed using the Normalized Difference Vegetation Index (NDVI), with images from Landsat 5 and Landsat 8, for the years 1987and 2017 5 Universidade Federal do Amazonas -UFAM 6 Cavalheiro Engenharia Rural e Empresarial Ltda. 7 Universidade Federal de Rondônia SOUZA FILHO et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…correcta identificação dos indivíduos, garante os direitos inerentes a essa personalidade, tornando-se assim, o ponto de partida da responsabilização das pessoas.Portanto, esta correcta identificação dos indivíduos materializa-se por meio da atribuição do denominado Bilhete de Identidade de Cidadão Nacional (B.I. )5 , que constitui o documento bastante para provar a nacionalidade e a identidade civil do indivíduo portador, perante quaisquer autoridades e entidades públicas ou privadas, nos termos da lei6 .…”

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Artigos Mais Citados E Mais Acessados Do Ano De 2023 Das Revistas Da Editora Recima21

Recima 21

2024

RECIMA21

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“…In 2007, Cannizzaro showed that O 3 enhanced the effects of cisplatin and etoposide on cultured neuroblastoma cells (13). Kuroda et al studied the effects of O 3 water on rectal cancer and concluded that topical application of O 3 water did not harm normal tissues; however, direct topical application of O 3 water to tumor tissues induced necrosis and inhibited tumor cell proliferation (14). Thus, O 3 has shown variable effects on different tumors in cell culture.…”

Section: Introductionmentioning

confidence: 99%

Ozone induces BEL7402 cell apoptosis by increasing reactive oxygen species production and activating JNK

Tang¹,

Xu²,

Li³

et al. 2021

Ann Transl Med

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Background: Oxidative stress is an important factor in the modulation of both tumorigenesis and anticancer responses. Ozone (O 3 ) is a strong oxidant that causes redox reactions and exerts anticancer effects in various types of cancer cells. However, the pathways involved in O 3 -induced cell death are not well understood. Methods: In vitro human hepatocellular carcinoma (HCC) BEL7402 cells were treated with various O 3 concentrations to evaluate O 3 cytotoxicity by Cell Counting Kit-8 (CCK-8) assay and flow cytometry. The regulatory mechanisms were analyzed by western blot analysis. In vivo, an HCC model was established to evaluate the inhibition of HCC with O 3 treatment. Results: In vitro cells treated with O 3 exhibited a round and small morphology with nuclear shrinkage and fragmentation. The CCK-8 assay confirmed the potent cytotoxic activity of O 3 against BEL7402 cells (IC 50 value of 5 μg/mL). Acridine orange/ethidium bromide (AO/EB) staining revealed apoptosis of BEL7402 cells after O 3 treatment. Flow cytometry analysis showed that S phase cell cycle arrest and apoptosis increased with O 3 exposure. In addition, O3 exposure reduced the mitochondrial membrane potential (ΔΨm) and induced reactive oxygen species (ROS) accumulation. Western blot analysis showed that O 3 exposure reduced B-cell lymphoma 2 (BCL-2) expression and increased cleaved poly ADP-ribose polymerase (PARP), cytochrome C (Cyt-C), caspase-3, caspase-9, and p-JNK expression. In vivo, treatment with intratumor injection O 3 (20 μg/mL) inhibited HCC growth.Conclusions: Overall, our findings showed that O 3 induces BEL7402 cell apoptosis via the intrinsic mitochondria-dependent pathway. Therefore, O 3 has therapeutic potential for HCC.

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Use of ozonated water as a new therapeutic approach to solve current concerns around antitumor treatment

Cited by 3 publications

References 21 publications

Immunotherapy Using Oxygenated Water and Tumor‐Derived Exosomes Potentiates Antitumor Immune Response and Attenuates Malignancy Tendency in Mice Model of Breast Cancer

Immunotherapy Using Oxygenated Water and Tumor‐Derived Exosomes Potentiates Antitumor Immune Response and Attenuates Malignancy Tendency in Mice Model of Breast Cancer

Artigos Mais Citados E Mais Acessados Do Ano De 2023 Das Revistas Da Editora Recima21

Ozone induces BEL7402 cell apoptosis by increasing reactive oxygen species production and activating JNK

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