Use of palladium(II) chloride as colour-forming reagent in determination of pralidoxime chloride in water and tablets

“…In order to improve the effect of AChR, we decided to modify obidoxime into the form of coordination compound, aiming to ensure slower decomposition of complex species and subsequently to increase the amount of the active compound in the organism. It is known that oxime-containing compounds react with metal ions to form complex species, yet these reactions were used mainly for quantitative determination of oximes in various formulations (Karljiković-Rajić et al , 1987 ; Karljiković-Rajić et al , 1988 ; Karljiković-Rajić et al , 1990 ; Korićanak et al , 1990 ; Karljiković-Rajić & Rajkovic, 1997 ). Moreover, to the best of our knowledge, systems containing metal ions and obidoxime have not been studied so far as to their potential ability to restore the activity of inhibited acetylcholinesterase.…”

Section: Discussionmentioning

confidence: 99%

Pd(II) complexes of acetylcholinesterase reactivator obidoxime

Nedzhib

Stoykova

Atanasov

et al. 2014

Interdisciplinary Toxicology

View full text Add to dashboard Cite

The ability of the acetylcholinesterase reactivator obidoxime (H2L2+) to bind palladium(II) cations was evaluated spectrophotometrically at different reaction conditions (pH, reaction time, metal-to-ligand molar ratio). The results showed that immediately after mixing the reagents, pH 7.4, complex species of composition [PdHL]3+ existed predominantly with a value of conditional stability constant lgβ‘=6.52. The reaction was completed within 24 hours affording the formation of species [Pd2L]4+ with significantly increased stability (lgβ‘=9.34). The spectral data suggest that obidoxime coordinates metal(II) ions through the oximate functional groups. The in vitro reactivation assay of paraoxon-inhibited rat brain acetylcholinesterase revealed that the new complex species were much less active than the non-coordinated obidoxime. The lack of reactivation ability could be explained by the considerable stability of complexes in solution as well as by the deprotonation of oxime groups essential for recovery of the enzymatic activity.

show abstract

“…Various methods have been reported for the determination of PAM-2, including high performance liquid chromatography [8][9][10] and spectrophotometric methods. 11 Chromatographic methods involve laborious and sophisticated procedures. Similarly, spectroscopic detection requires complex formation to enhance the sensitivity and selectivity of the technique which hampers its suitability for routine analysis.…”

Section: Introductionmentioning

confidence: 99%

Stripping voltammetric determination of pyridine-2-aldoxime methochloride at the iron(iii) doped zeolite modified glassy carbon electrode

Mehretie

Losada

Tessema

et al. 2012

Analyst

View full text Add to dashboard Cite

An iron(III) doped zeolite modified glassy carbon electrode was constructed for the determination of pyridine-2-aldoxime methochloride. X-ray diffraction and chemical analysis were utilized to determine the optimum pH and chemical content for doping zeolite. Cyclic voltammetry was used to characterize the modified electrode and study the kinetics of the acid treated and untreated modified electrode. Acid treatment of the modified electrode showed a better electrochemical behavior compared to the untreated iron(III) doped zeolite modified electrode. Square wave anodic stripping voltammetry was employed to investigate the working pH and preconcentration time. The analytical performance of the modified electrode was evaluated, and a linear anodic stripping response for pyridine-2-aldoxime methochloride in the concentration range of 0.5-100.0 μM with a detection limit of 1.61 × 10(-7) M was obtained. Finally, the developed method was successfully applied for the determination of pyridine-2-aldoxime methochloride in a biological sample.

show abstract

Use of palladium(II) chloride as colour-forming reagent in determination of pralidoxime chloride in water and tablets

Cited by 11 publications

References 14 publications

Vibrational analysis of 1-methyl-pyridinium-2-aldoxime and 1-methyl-pyridinium-4-aldoxime cations

Vibrational analysis of 1-methyl-pyridinium-2-aldoxime and 1-methyl-pyridinium-4-aldoxime cations

Pd(II) complexes of acetylcholinesterase reactivator obidoxime

Stripping voltammetric determination of pyridine-2-aldoxime methochloride at the iron(iii) doped zeolite modified glassy carbon electrode

Contact Info

Product

Resources

About